The Effect of Barbituric Acid Derivatives on Platelet Function in Vitro and in Vivo

J. Heinz Joist; Jean-Pierre Cazenave; J. Fraser Mustard

doi:10.1055/s-0038-1649080

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(02): 315-326
DOI: 10.1055/s-0038-1649080

Original Article

Schattauer GmbH

The Effect of Barbituric Acid Derivatives on Platelet Function in Vitro and in Vivo^[*]

J. Heinz Joist

¹Department of Pathology, McMaster University, Hamilton, Ontario, Canada

²Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

,

Jean-Pierre Cazenave

¹Department of Pathology, McMaster University, Hamilton, Ontario, Canada

²Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

,

J. Fraser Mustard

¹Department of Pathology, McMaster University, Hamilton, Ontario, Canada

²Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada

› Author Affiliations

Abstract

Summary

Sodium pentobarbital (SPB) and three other barbituric acid derivatives were found to inhibit platelet function in vitro. SPB had no effect on the primary response to ADP of platelets in platelet-rich plasma (PRP) or washed platelets but inhibited secondary aggregation induced by ADP in human PRP. The drug inhibited both phases of aggregation induced by epinephrine. SPB suppressed aggregation and the release reaction induced by collagen or low concentrations of thrombin, and platelet adherence to collagen-coated glass tubes. The inhibition by SPB of platelet aggregation was readily reversible and isotopically labeled SPB did not become firmly bound to platelets. No inhibitory effect on platelet aggregation induced by ADP, collagen, or thrombin could be detected in PRP obtained from rabbits after induction of SPB-anesthesia.

Full Text

References

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The Effect of Barbituric Acid Derivatives on Platelet Function in Vitro and in Vivo[*]

The Effect of Barbituric Acid Derivatives on Platelet Function in Vitro and in Vivo^[*]