Leucocytes and Thrombosis

D.C Banks; J.R.A Mitchell

doi:10.1055/s-0038-1649101

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(01): 047-061
DOI: 10.1055/s-0038-1649101

Original Article

Schattauer GmbH

Leucocytes and Thrombosis

II. Relationship between Leucocyte Behaviour and Divalent Cations, Sulphydryl Groups, Red Cells and Adenosine Diphosphate

Authors

D.C Banks

¹Department of Medicine, University of Nottingham, England
J.R.A Mitchell

¹Department of Medicine, University of Nottingham, England

Abstract

Summary

The rate of white cell loss from heparinised whole blood in a rotating glass flask has been used to study leucocyte behaviour.

Leucocyte loss requires divalent cations and magnesium is more active than calcium. Sulphydryl blockers and potassium cyanide inhibit white cell loss, showing that sulphydryl groups and a glycolytic pathway play a part in leucocyte adhesion.

White cell loss from plasma rotated in a flask is negligible, but is markedly increased by adding red cells, red cell sonicates or ADP.

The role of platelets and of substances which affect platelet behaviour in white cell adhesion is discussed.

Full Text

References

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