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Thromb Haemost 1973; 30(01): 047-061
DOI: 10.1055/s-0038-1649101
Original Article
Schattauer GmbH

Leucocytes and Thrombosis

II. Relationship between Leucocyte Behaviour and Divalent Cations, Sulphydryl Groups, Red Cells and Adenosine Diphosphate
D.C Banks
1   Department of Medicine, University of Nottingham, England
,
J.R.A Mitchell
1   Department of Medicine, University of Nottingham, England
› Author Affiliations
Further Information

Publication History

Received for publication 11 April 1973

Accepted for publication 06 June 1973

Publication Date:
30 June 2018 (online)

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Summary

The rate of white cell loss from heparinised whole blood in a rotating glass flask has been used to study leucocyte behaviour.

Leucocyte loss requires divalent cations and magnesium is more active than calcium. Sulphydryl blockers and potassium cyanide inhibit white cell loss, showing that sulphydryl groups and a glycolytic pathway play a part in leucocyte adhesion.

White cell loss from plasma rotated in a flask is negligible, but is markedly increased by adding red cells, red cell sonicates or ADP.

The role of platelets and of substances which affect platelet behaviour in white cell adhesion is discussed.

 

  • References

  • 1 Aledort L. M, Troup S. B, and Weed R. I. 1968; Inhibition of sulphydryl-dependent platelet functions by penetrating and non-penetrating analogues of parachloromercuribenzene. Blood 31: 471.
    PubMedSearch in Google Scholar
  • 2 Banks D. C, and Mitchell J. R. A. 1973; Leucocytes and thrombosis. I. A simple test of leucocyte behaviour. Thrombosis et Diathesis Haemorrhagica 30: 36.
    PubMedSearch in Google Scholar
  • 3 Bishop C, Rankine D. M, and Talbott J. H. 1959; The nucleotides in normal human blood. Journal of Biological Chemistry 234: 1233.
    PubMedSearch in Google Scholar
  • 4 Böyum A. 1968 Separation of leucocytes from blood and bone marrow. Scandinavian Journal of Clinical Investigation. 21, Suppl. 97.
    PubMedSearch in Google Scholar
  • 5 Evans C. A, and Middleton V. L. 1970; Evaluation of methods for preparing pure lymphocyte suspensions from peripheral blood. Journal of Clinical Pathology 23: 822.
    CrossrefPubMedSearch in Google Scholar
  • 6 Fisher T. N, and Ginsberg H. S. 1956; The reaction of influenza viruses with guinea pig polymorphonuclear leucocytes. 11. The reduction of white blood cell glycolysis by influenza viruses and receptor-destroying enzyme (RDE). Virology 2: 637.
    CrossrefPubMedSearch in Google Scholar
  • 7 Friedberg N. M, and Zucker M. B. 1971 ADP as the cause of reversible inhibition of platelet retention. Information exchange, 71-44.
    PubMedSearch in Google Scholar
  • 8 Gaarder A, Jonsen J, Laland S, Hellem A, and Owren P. A. 1961; Adenosine diphosphate in red cells as a factor in the adhesiveness of human blood platelets. Nature 192: 531.
    CrossrefPubMedSearch in Google Scholar
  • 9 Garvin J. E. 1961; Factors affecting the adhesiveness of human leucocytes and platelets in vitro. Journal of Experimental Medicine 114: 51.
    CrossrefPubMedSearch in Google Scholar
  • 10 Hampton J. R, and Mitchell J. R. A. 1966; Effect of glass contact on the electrophoretic mobility of human blood platelets. Nature 209: 470.
    CrossrefPubMedSearch in Google Scholar
  • 11 Harrison M. J. G, Emmons P. R, and Mitchell J. R. A. 1966; The effect of sulphydryl and enzyme inhibitors on platelet aggregation in vitro. Thrombosis et Diathesis Haemorrhagica 16: 122.
    PubMedSearch in Google Scholar
  • 12 Harrison M. J. G, and Mitchell J. R. A. 1966; The influence of red blood cells on platelet adhesiveness. Lancet 2: 1163.
    PubMedSearch in Google Scholar
  • 13 Haslam R. J. 1964; Role of adenosine diphosphate in the aggregation of human blood platelets by thrombin and by fatty acids. Nature 202: 765.
    CrossrefPubMedSearch in Google Scholar
  • 14 Hellem A. J. 1960 The adhesiveness of human blood platelets in vitro. Scandinavian Journal of Clinical Investigation. 12, Suppl. 51.
    PubMedSearch in Google Scholar
  • 15 Kvarstein B. 1969; a Effects of proteins and inorganic ions on the adhesiveness of human leucocytes to glass beads. Scandinavian Journal of Clinical and Laboratory Investigation 24: 41.
    CrossrefPubMedSearch in Google Scholar
  • 16 Kvarstein B. 1969; b Effect of some metabolic inhibitors on the adhesiveness of human leucocytes to glass beads. Scandinavian Journal of Clinical and Laboratory Investigation 24: 35.
    CrossrefPubMedSearch in Google Scholar
  • 17 Mitchell J. R. A, and Schwartz C. J. 1965. Arterial disease. Blackwell Scientific; Oxford:
    Search in Google Scholar
  • 18 Mitchell J. R. A, and Sharp A. A. 1964; Platelet clumping in vitro. British Journal of Haematology 10: 78.
    CrossrefPubMedSearch in Google Scholar
  • 19 Robinson C. W, Mason R. G, and Wagner R. H. 1963; Effect of sulphydryl inhibitors on platelet agglutinability. Proceedings of the Society of Experimental Biology, New York 116: 857.
    PubMedSearch in Google Scholar
  • 20 Tyrode M. V. 1909; The mode of action of some purgative salts. Archives internationales de pharmacodynamic et de therapie 19: 205.
    PubMedSearch in Google Scholar