Effect of Aspirin and Benadryl on Human Platelet Oxidative Phosphorylation and Aggregation

Kenneth T. N. Yue; James W. Davis; E. Gray Aldridge

doi:10.1055/s-0038-1649136

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1973; 30(03): 577-585
DOI: 10.1055/s-0038-1649136

Original Article

Schattauer GmbH

Effect of Aspirin and Benadryl on Human Platelet Oxidative Phosphorylation and Aggregation

Authors

Kenneth T. N. Yue

¹Hematology Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

²Departments of Pathology and Medicine, University of Kansas School of Medicine, Kansas City, Kansas 66103
James W. Davis

¹Hematology Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

²Departments of Pathology and Medicine, University of Kansas School of Medicine, Kansas City, Kansas 66103
E. Gray Aldridge

¹Hematology Research Laboratory, Veterans Administration Hospital, Kansas City, Missouri 64128

²Departments of Pathology and Medicine, University of Kansas School of Medicine, Kansas City, Kansas 66103

Abstract

Summary

Tightly coupled oxidative phosphorylation in washed human platelets and in lysates has been demonstrated. Both aspirin (25 μM) and Benadryl (0.15 mM) inhibited phosphorylating oxidation but had no effect on uncoupled respiration. It is likely that they were not involved in the electron transport chain but specifically affected the phosphorylation reactions similar to the action of oligomycin. Preincubation of Benadryl (1.3 mM) with platelet-rich plasma (PRP) at room temperature for 2 min inhibited ADP-induced platelet aggregation by 62% and abolished the second phase of aggregation. Addition of Benadryl to aggregating platelets in PRP during ADP-induced aggregation caused immediate disaggregation whether added during the first or second phase of aggregation. Aspirin had no such effect.

Full Text

References

References
1 Al-Mondhiry H, Marcus A. J, and Spaet T. H. 1970; On the mechanism of platelet function inhibition by acetylsalicylic acid. Proceedings of the Society for Experimental Biology and Medicine 133: 630.
2 Ardlie N. G, Packham M. A, and Mustard J. F. 1970; Adenosine diphosphate-induced platelet aggregation in suspensions of washed rabbit platelets. British Journal of Haematology 19: 7.
3 Barber A. J, and Jamieson G. A. 1970; Isolation and characterization of plasma membranes from human blood platelets. Journal of Biological Chemistry 245: 6357.
4 Brody T. M. 1956; Action of sodium salicylate and related compounds on tissue metabolism in vitro. Journal of Pharmacology and Experimental Therapeutics 117: 39.
5 Bruni A, Contessa A. R, and Scalella P. 1965; The binding of atractyloside and oligomycin to liver mitochondria. Biochimica et Biophysica Acta 100: 1.
6 Chernyak N. B. 1965; Processes of formation and utilization of energy in human blood platelets. Clinica Chimica Acta 12: 244.
7 Doery J. C. G, Hirsh J, and De Gruchy G. C. 1969; Aspirin: Its effect on platelet glycolysis and release of adenosine diphosphate. Science 165: 65.
8 Doery J. C. G, Hirsh J, and Cooper I. 1970; Energy metabolism in human platelets: Interrelationship between glycolysis and oxidative metabolism. Blood 36: 159.
9 Holmsen H, Day H. J, and Stormorken H. 1969 The blood platelet release reaction. Scandinavian Journal of Haematology, Supplementum No. 8.
10 Kinlough R. L, Packham M. A, and Mustard J. F. 1969; Glucose and platelet aggregation. Federation Proceedings 28: 509.
11 Lowry O. H, Rosebrough N. J, Farr A. L, and Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193: 265.
12 Macmillan D. C. 1966; Secondary clumping effect in human citrated platelet-rich plasma produced by adenosine diphosphate and adrenaline. Nature 211: 140.
13 McElroy F. A, Kinlough-Rathbone R. L, Ardlie N. G, Packham M. A, and Mustard J. F. 1971; The effect of aggregating agents on oxidative metabolism of rabbit platelets. Biochimica et Biophysica Acta 253: 64.
14 Mills D. C. B, and Roberts G. C. K. 1967; Membrane active drugs and the aggregation of human blood platelets. Nature 213: 35.
15 Mürer E. H. 1968; Release reaction and energy metabolism in blood platelets with special reference to the burst in oxygen uptake. Biochimica et Biophysica Acta 162: 320.
16 O’Brien J. R. 1968; Effects of salicylates on human platelets. Lancet 1: 779.
17 O’Brien J. R. 1961; The adhesiveness of native platelets and its prevention. Journal of Clinical Pathology 14: 140.
18 Rock R. C, and Nemerson Y. 1969; Energetics of human blood platelets: Uncoupling of oxidative phosphorylation in intact cells. Journal of Laboratory and Clinical Medicine 73: 42.
19 Weiss H. J, Aledort L. M, and Kochwa S. 1968; The effect of salicylates on the hemostatic properties of platelets in man. Journal of Clinical Investigation 47: 2169.
20 Zucker M. B, and Peterson J. 1968; Inhibition of adenosine diphosphate-induced secondary aggregation and other platelet functions by acetylsalicylic acid ingestion. Proceedings of the Society for Experimental Biology and Medicine 127: 547.