Download PDF
Thromb Haemost 1974; 31(03): 452-456
DOI: 10.1055/s-0038-1649185
Original Article
Schattauer GmbH

Measurement of the Rate of Clot Retraction

Michio Itoh
1   Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, Japan
,
Yoshihisa Nishimura
1   Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, Japan
,
Osamu Takenaka
1   Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, Japan
,
Yuji Inada
1   Laboratory of Biological Chemistry, Tokyo Institute of Technology, Ookayama, Meguro-ku, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

Received 04 January 1974

Accepted 18 April 1974

Publication Date:
29 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

The volume of serum exuded from clot was measured with time in the presence of platelets. The change of the volume of clot followed the first order kinetics with respect to time and the rate constant (k) was introduced to assay for clot retraction. The value of k and maximal serum volume exuded from clot, Vf, are dependent on the number of platelets, but the way k depends on the number of platelets is different from that Vf does on it. This suggests that these two parameters reflect the different phases of clot retraction.

 

  • References

  • 1 Aster R. H, Jandl J. H. Platelet sequestration in man. I. Methods. Journal of Clinical Investigation 1964; 43: 843
    CrossrefPubMedSearch in Google Scholar
  • 2 Budtz-Olsen O. E. 1951. Clot retraction . Oxford.: Blackwell Scientific Publications;
    Search in Google Scholar
  • 3 Degaetano G, Donati M. B, Vermylen J, Verstraete M. Inhibition of clot retraction by previous in vitro platelet aggregation. Thrombosis et Diathesis Haemorrhagica 1971; 26: 449
    PubMedSearch in Google Scholar
  • 4 MacFarlane R. G. The normal haemostatic mechanism and its failure in the haemor- rhagic states. Thesis for the Degree of Doctor of Medicine in the University of London. 1938
    Search in Google Scholar
  • 5 Majno G, Bouvier C. A, Gabbiani G, Ryan G. B, Statkov P. Kymographic recording of clot retraction : Effects of papverine theophylline and cytochalasin B. Thrombosis et Diathesis Haemorrhagica 1972; 28: 49
    PubMedSearch in Google Scholar
  • 6 Marchal G, Leroux M. E, Samama M. Atlas de thrombodynamographie. . 1 vol., S. P. E., I. Paris.: 1962
    Search in Google Scholar
  • 7 Shepro D, Belamarich F. A, Chao F. C. Retardation of clot retraction after incubation of platelets with colchicine and heavy water. Nature 1969; 221: 563
    CrossrefPubMedSearch in Google Scholar