Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1968; 19(01/02): 221-225DOI: 10.1055/s-0038-1651199 Originalarbeiten – Original Articles – Travaux Originaux Schattauer GmbH Inactivation of Tissue Thromboplastin by Ultraviolet Irradiation S Åtland 1 Departments of Physics and Microbiology, University of Oslo, Blindern, Oslo 3, Norway , M Hvatum * 1 Departments of Physics and Microbiology, University of Oslo, Blindern, Oslo 3, Norway , H Prydz 1 Departments of Physics and Microbiology, University of Oslo, Blindern, Oslo 3, Norway › Author Affiliations Recommend Article Abstract Buy Article Summary Solutions of partially purified tissue thromboplastin have been irradiated with UV light of different wavelengths, and the resulting reduction in the procoagulant activity has been measured. The activity decreased as an exponential function of the dose. The action spectrum obtained was closely similar to the UV absorption spectrum of the thromboplastic preparations and had a maximum between 270-290 nm. The data suggested that the reduction in the procoagulant activity was caused by absorption of radiation energy in the protein part of the thromboplastic structure. Full Text References References 1 Hvatum M, Prydz H. Studies on tissue thromboplastin. I. Solubilization with sodium deoxy-cholate. Biochim. biophys. Acta (Amst.) 1966; 130: 92 2 Wooldridge L.C. On the chemistry of the blood other scientific papers. p. 135. Kegan Paul Trench Trübner London Cit. E.C., Hecht 1893 7 3 Mills C.A. Chemical nature of tissue coagulins. J. biol. Chem. 1921; 46: 135 4 Cohen S.S, Chargaff E. Studies on the chemistry of blood coagulation. IX. The thrombo-plastic protein from lungs. J. biol. Chem. 1940; 136: 243 5 Studer A. Festschrift Barell. Hoffmann-La Roche, Basel p. 229. Cit. E. C. Hecht 1946 7 6 Deutsch E, Irsigler K, Lomoschitz H. Studien über Gewebethromboplastin 1. Reinigung, chemische Charakterisierung und Trennung in einen Eiweiß- und Lipoidanteil. Thrombos. Diathes. haemorrh. (Stuttg.) 1964; 12: 12 7 Hecht E.C. Lipids in blood clotting. Charles C. Thomas; Springfield USA: 1965: 328 8 Hecht E. C: Chemical nature of human brain thromboplastin. Nature (Lond.) 1967; 214: 197 9 Hecht E.C, Cho M.H, Seegers W.H. Thromboplastin: Nomenclature and preparation of protein-free material different from platelet factor 3 or lipid activator. Amer. J. Physiol. 1958; 193: 584 10 Lowry O.H, Rosebrough N.J, Farr A.L, Randall R.J. Protein measurement with the Folin phenol reagent. J. biol. Chem. 1951; 193: 265 11 Chen Jr. P.S, Toribara T.Y, Warner H. Microdetermination of phosphorus. Anal. Chem. 1956; 28: 1756 12 Kabat E.A, Mayer M.M. Experimental Immunochemistryg. Charles C. Thomas; Springfield, Illionois: 1961: 505 13 Svennerholm L. Quantitative estimation of sialic acid with the resorcinol-HCl method. Biochim. biophys. Acta (Amst.) 1957; 24: 604 14 Gibbons M.N. The determination of methylpentoses. Analyst 1955; 90: 268
