The Modification of Experimental Occlusive Arterial Thrombosis in the Rat by Malayan Pit Viper Venom

K. E Chan

doi:10.1055/s-0038-1651202

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1968; 19(01/02): 242-247
DOI: 10.1055/s-0038-1651202

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

The Modification of Experimental Occlusive Arterial Thrombosis in the Rat by Malayan Pit Viper Venom

K. E Chan M. B., B. S., Ph. D

¹Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

› Author Affiliations

Abstract

Summary

The effect of Malayan pit viper (Ancistrodon rhodostoma) venom on the fate of experimental arterial thrombosis was studied in rats. A suitable daily dose of venom (500 μg) was used to induce hypofibrinogenaemia in the treated rats for the greater part of each of three consecutive post-operative days.

The treated animals showed a statistically significant overall reduction in the incidence of both red thrombus formation and thrombotic arterial occlusion when compared to a control group. This antithrombotic effect of the venom could be observed in the 7-day period following the cessation of the treatment.

Full Text

References

References
1 Chan K.E. Experimental production of acute arterial thrombosis in the carotid artery of the rat. Thrombos. Diathes. haemorrh. (Stuttg.).(In press.).
2 Reid H.A, Chan K.E, Thean P.C. Prolonged coagulation defect (defibrination syndrome) in Malayan viper bite. Lancet 1963; I: 621
3 Chan K.E. The hypofibrinogenaemic state in Malayan viper poisoning. Doctorial Thesis.. The University of St. Andrews; Scotland: 1964
4 Regoeczi E, Gergely J, McFarlane A.S. In: vivo effects of Agkistrodon rhodostoma venom: Studies with fibrinogen ¹³¹I. J. clin. Invest. 1966; 45: 1203
5 Chan K.E. Relation between haemostasis and fibrinogen in Genetics and the interaction of blood clotting factors.. ed Koller F, Streuli F. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl. 1965: 134
6 Marsten J.L, Chan K.E, Ankeney J.L, Botti R.E. Antithrombotic effect of Malayan pit viper venom on experimental thrombosis of the inferior vena cava produced by a new method. Circulat. Res. 1966; 19: 514
7 Sharp A.A, Howie B, Biggs R, Methuen D.T. Defibrination syndrome in pregnancy — value of various diagnostic tests. Lancet 1958; II: 1309
8 Oettle A.G, Spriggs A.I. New optical and cytochemical techniques in haematology. p 406. Recent Advances in Haematology.. ed Dyke S.C. J. & A. Churchill Ltd; London: 1951
9 Chan K.E, Rizza C.R, Hendersen M.P. A study of the coagulant properties of Malayan pit viper venom. Brit. J. Haemat. 1965; 11: 646
10 Honour A.J, Ross Russel R.W. Experimental platelet embolism. Brit. J. exp. Path. 1962; 43: 350
11 Fulton G.P, Akers R.P, Lutz B.R. White thromboembolism and vascular fragility in the hamster cheek pouch after anticoagulants. Blood 1953; 8: 140
12 Mustard J.F, Murphy E.A, Downie H.G, Roswell H.C. Heparin and thrombus formation : Early suppression and late enhancement. Brit. J. Haemat. 1963; 9: 548
13 Kiesewetter W.B, Schumacher A.B. An experimental study of the comparative efficacy of heparin and dicumarol in the prevention of arterial and venous thrombosis. Surg. Gynec. Obstet. 1948; 86: 687
14 Williams R.D, Carey L.C. Studies in the production of standard venous thrombosis. Ann. Surg. 1959; 149: 381
15 Zweifler A.J. Standardised technique for the study of thrombus growth: Effect of heparin therapy. J. Lab. clin. Med. 1962; 60: 254