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Thromb Haemost 1969; 22(01): 110-120
DOI: 10.1055/s-0038-1651332
Originalarbeiten-Original Articles-Travaux Originaux
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The Determination of Serum Fibrinolytic Inhibitors in Normal Practice

J Daver
1   Laboratoire de Biologie, Centre Hospitalier, Castres (81) France
,
M. J Albugues
1   Laboratoire de Biologie, Centre Hospitalier, Castres (81) France
,
J. P. Du Pasquier
2   C. E. I. P., 195, route d’Espagne, Toulouse (31) France
,
M Ricoeur
2   C. E. I. P., 195, route d’Espagne, Toulouse (31) France
› Author Affiliations
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Publication Date:
10 June 2018 (online)

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Summary

It is desirable that every biology laboratory should be able to carry out the determination of serum fibrinolytic inhibitors. Two simple techniques which can be easily performed have been studied.

The first consists in measuring the lysis surface, using an activator, (plasmin) of a fibrin plate, to which the inhibitors are added in the form of a 1/10th concentration of the serum to be studied, using Blix’s method.

The second is the measuring of a standard fibrin clot, activated by streptokinase, in the presence of added inhibitors in the form of a 1/10th concentration of the serum to be studied, using Hawkey’s method.

The measurements taken by double determination with these two techniques on the sera of 569 subjects during epidemiological research on atherosclerosis, have shown that they do not measure the same thing, and it is not possible to give an explanation. However it is possible to make some assumptions.

 

  • References

  • 1 Albrechtsen O. K. The fibrinolytic activity of human tissues. Brit. J. Haemat. 3: 284-291 1957;
    CrossrefPubMedSearch in Google Scholar
  • 2 Albugues M. J. Contribution à l’étude des inhibiteurs sériques de la fibrinolyse. Thèse Méd.; Toulouse: 1968
    Search in Google Scholar
  • 3 Alkjaersig N, Fletcher A. P, Sherry S. Activation of plasminogen. II. A kinetic study of activation with trypsin urokinase and streptokinase. J. biol. Chem. 233: 86-90 1958;
    PubMedSearch in Google Scholar
  • 4 Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. 1952; 40: 346-351.
    CrossrefPubMedSearch in Google Scholar
  • 5 Blix S. The quantitative determination of fibrinolytic inhibitors in plasma or serum by means of the fibrin plate. Scand. J. clin. Lab. Invest. 16: 403-408 1964;
    CrossrefPubMedSearch in Google Scholar
  • 6 Christensen L. P. Methods for measuring the activity of components of the streptococcal fibrinolytic system and streptococcal desoxyribonuclease. J clin. Invest. 16: 403-408 1949;
    PubMedSearch in Google Scholar
  • 7 Daver J, Albugues M. J, Queritet A, Messerli P. Le système inhibiteur de la fibrinolyse. Rev. Med. Toulouse (à paraître).
    PubMed
  • 8 Freiman A. H. Factors affecting the formation and dissolution of experimental thrombi. Amer. J. Cardiol. 6: 426-431 1960;
    CrossrefPubMedSearch in Google Scholar
  • 9 Gormsen J. Fibrinolytic activity: inhibitors of plasminogen activation. Acta med. Scand. 172: 657-663 1962;
    PubMedSearch in Google Scholar
  • 10 Gallimore M. J, Shaw J. T. B. Some aspects of fibrin clot lysis and its inhibition by human serum. Thrombos. Diathes. haemorrh. (Stuttg.) 18: 101-113 1967;
    PubMedSearch in Google Scholar
  • 11 Hawkey C. M, Stafford J. L. Standard clot method for assay of plasminogen activators, anti-activators and plasmin. J. clin. Path. 17: 175-181 1964;
    CrossrefPubMedSearch in Google Scholar
  • 12 Kawano T, Kazuo M, Yashiro V. Urokinase in human placenta. Nature (Lond.) 217: 253-254 1968;
    CrossrefPubMedSearch in Google Scholar
  • 13 Lighbown J. W, Sulitzeanu D. The assay of penicillin in blood serum using sarcina lutea. Bull. Who. 17: 553-567 1957;
    PubMedSearch in Google Scholar
  • 14 Mounier J, Bourse R. Personal communication.
    PubMed
  • 15 Perlick E, Deutschinoff A. Activité fibrinolytique de la tunique vasculaire artérioscléreuse. Hémostase 1: 249-254 1961;
    PubMedSearch in Google Scholar
  • 16 Remmert L. F, Cohen P. P. Partial purification and properties of a proteolytic enzyme of human serum. J. biol. Chem. 181: 431-448 1949;
    PubMedSearch in Google Scholar
  • 17 Vilalta J. Contribution à l’étude épidémiologique de l’athérosclérose. (Premiers résultats d’une enquête portant sur 857 personnes). Thèse Méd. Toulouse, 1968.
    PubMed