Soluble Fibrin Monomer Complexes Demonstrated by Agarose Gel Filtration and by Adsorption on Insolubilized Fibrinogen

R von Hugo; R Hafter; A Stemberger; H Graeff

doi:10.1055/s-0038-1651369

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1975; 34(01): 216-222
DOI: 10.1055/s-0038-1651369

Original Article

Schattauer GmbH

Soluble Fibrin Monomer Complexes Demonstrated by Agarose Gel Filtration and by Adsorption on Insolubilized Fibrinogen

A Comparative Study

R von Hugo

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

,

R Hafter

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

,

A Stemberger

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

,

H Graeff

¹I. Universitäts-Frauenklinik (Head: Prof. Dr. J. Zander), Munich University, Maistrasse 11, 8 München 2, Germany

²Max-Planck-Institut für Biochemie, 8033 Martinsried b. München, Germany

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Abstract

Summary

Incubation of fibrinogen with small amounts of thrombin resulted in the occurrence of soluble fibrin monomer complexes. These complexes consisted predominantly of a derivative with a higher molecular weight than that of fibrinogen. It was characterized by its relative electrophoretic mobility in 5% PAA gel (0.28 × 10^-5 cm²/V × sec) and its elution position prior to the fibrinogen peak following gel filtration. Using adsorption chromatography on insolubilized fibrinogen the derivative dissociated at a ratio of almost 1 : 1 into one part which was adsorbed and into fibrinogen which was not adsorbed. The part which was adsorbed seemed to be the thrombin mediated fibrin monomer. This study confirms the concept that dissociable dimeric fibrinogen-fibrin monomer complexes occur after limited action of thrombin on fibrinogen.

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Referenzen

References
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