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Thromb Haemost 1969; 22(03): 496-507
DOI: 10.1055/s-0038-1651386
Originalarbeiten-Original Articles-Travaux Originaux
Schattauer GmbH

Accumulation of Macromolecular Particles in the Reticuloendothelial System (RES) Mediated by Platelet Aggregation and Disaggregation

W.G van Aken
1   Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands
,
J Vreeken Amsterdam, The Netherland
1   Central Laboratory of The Netherlands Red Cross Blood Transfusion Service, Amsterdam, The Netherlands
› Author Affiliations
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Publication History

Publication Date:
10 June 2018 (online)

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Summary

Carbon particles cause platelet aggregation in vitro and in vivo. Prior studies established that substances which modify thrombocyte aggregation also influence the rate at which carbon is cleared from the blood.

This study was performed in order to elucidate the mechanism by which the carbon-platelet aggregates specifically accumulate in the RES.

Activation of fibrinolysis by urokinase or streptokinase reduced the carbon clearance rate, probably due to generated fibrinogen degradation products (FDP). Isolated FDP decreased the carbon clearance and caused disaggregation of platelets and particles in vitro. Inhibition of fibrinolysis by epsilon-amino-caproic acid (EACA), initially accelerated the disappearance of carbon and caused particle accumulation outside the RES, predominantly in the lungs. It is supposed that platelet aggregation and locally activated fibrinolysis act together in the clearance of particles. In the normal situation the RES with its well known low fibrinolytic activity, becomes the receptor of the particles.

 

  • References

  • 1 Tait J, Elvidge A. R. Effect upon platelets and on blood coagulation of injecting foreign particles into the bloodstream. J. Physiol. 62: 129 1926;
    CrossrefPubMedSearch in Google Scholar
  • 2 Salvidio E, Crosby W. H. Thrombocytopenia after intravenous injection of India ink. J. Lab. clin. Med. 56: 111 1960;
    PubMedSearch in Google Scholar
  • 3 Stehbens W. E, Florey H. W. The behaviour of intravenously injected particles in chambers in rabbits’ ears. Quart. J. exp. Physiol. 45: 252 1960;
    PubMedSearch in Google Scholar
  • 4 Cohen P, Braunwald J, Gardner F. H. Destruction of canine and rabbit platelets, following intravenous administration of carbon particles or endotoxin. J. Lab. clin. Med. 66: 263 1965;
    PubMedSearch in Google Scholar
  • 5 French J. E. Blood platelets: Morphological studies on their properties and life cycle. Brit. J. Haemat. 25: 595 1967;
    PubMedSearch in Google Scholar
  • 6 Van Aken W. G, Goote Th. M, Vreeken J. Platelet aggregation: An intermediary mechanism in carbon clearance. Scand. J. Haemat. 5: 333 1968;
    PubMedSearch in Google Scholar
  • 7 Aschoff L. Morphologie des reticuloendothelialen Systems. In: Handbuch der Krankheiten des Blutes und der blutbildenden Organe. Enzyklopaedie der klinischen Medizin, Spezieller Teil. Hsgb. Schnittenhelm A. Springer Verlag; Berlin: 2 473 1925
    Search in Google Scholar
  • 8 Halpern B. N, Benacerraf B, Biozzi G. Quantitative study of the granulopectic activity of the reticuloendothelial system. Brit. J. exp. Path. 34: 426 1953;
    PubMedSearch in Google Scholar
  • 9 Biozzi G, Benacerraf B, Holpern B. N. Quantitative study of the granulopectic activity of the reticuloendothelial system. Brit. J. exp. Path. 34: 441 1953;
    PubMedSearch in Google Scholar
  • 10 Kazal L. A, Amsel S, Miller D. P, Tocantins L. M. The preparation and some properties of fibrinogen precipitated from human plasma by glycine. Proc. Soc. exp. Biol. (N. Y.) 113: 989 1963;
    CrossrefPubMedSearch in Google Scholar
  • 11 Kline D. L, Fishman J. B. Improved procedure for the isolation of human plasminogen. J. biol. Chem. 236: 3233 1961;
    PubMedSearch in Google Scholar
  • 12 Clauss A. Gerinnungsphysiologische Schnellmethode zur Bestimmung des Fibrinogens. Acta haemat. 17: 237 1957;
    CrossrefPubMedSearch in Google Scholar
  • 13 Strengers Th, Asberg E. G. M. Een screening test, gevolgd door een snelle kwantitatieve microbepaling van fibrinogeen in plasma. Ned. T. Geneesk. 107: 2044 1963;
    PubMedSearch in Google Scholar
  • 14 French P. A. The coagulation of blood. Investigations on a new clotting factor. Acta med. scand. Suppl. ad 128: 194 1947;
    PubMedSearch in Google Scholar
  • 15 Stormorken H. The preparation of proaccelerin deficient (parahemophilia) plasma for the assay of proaccelerin. Scand. J. clin. Lab. Invest. 9: 273 1957;
    CrossrefPubMedSearch in Google Scholar
  • 16 Remmert L. F, Cohen P. Partial purification and properties of proteolytic enzyme of human plasma. J. biol. Chem. 181: 431 1949;
    PubMedSearch in Google Scholar
  • 17 Alkjaersig N, Fletcher A. P, Sherry S. The mechanism of clot dissolution by plasmin. J. clin. Invest. 38: 1086 1959;
    CrossrefPubMedSearch in Google Scholar
  • 18 Peetoom F. Over de herkenning en anatyse van antigene bestanddelen in menselijk bloed met behulp van agar-precipitatie technieken. Thesis; Amsterdam: 1961
    Search in Google Scholar
  • 19 Born G. V. R. Quantitative investigations into the aggregation of blood platelets. J. Physiol. 162: 67P 1962;
    PubMedSearch in Google Scholar
  • 20 O’Brien J. R. Platelet aggregation : Part II Some results from a new method of study. J. clin. Pathol. 75: 452 1962;
    PubMedSearch in Google Scholar
  • 21 Marcus A. J, Zucker M. B. The Physiology of Blood Platelets. Editors Grune and Stratton; New York: 1965
    Search in Google Scholar
  • 22 Brinkhous K. M, Wright I. S, Soldier J. P, Roberts H. R, Hinnom S. Platelets: Their role in hemostasis and thrombosis. F. K. Schattauer Verlag; Stuttgart: 1967
    Search in Google Scholar
  • 23 Johnson S.A, Seegers W. H. Physiology of haemostasis and thrombosis. C. C. Thomas; Springfield: 1967
    Search in Google Scholar
  • 24 Kwaan H. C, McFadzean A. J. S. On plasma fibrinolytic activity induced by ischaemia. Clin. Sci. 75: 245 1956;
    PubMedSearch in Google Scholar
  • 25 Tagnon H. J, Levenson S. M, Davidson C. S, Taylor F. H. L. The occurence of fibrinolysis in shock, with observations on the prothrombin time and the plasma fibrinogen during haemorrhagic shock. Amer. J. med. Sci. 211: 88 1946;
    CrossrefPubMedSearch in Google Scholar
  • 26 Astrup T, Albrechtsen O. K. Estimation of the plasminogen activator and the trypsin inhibitor in animal and human tissues. Scand. J. clin. Lab. Invest. 9: 233 1957;
    CrossrefPubMedSearch in Google Scholar
  • 27 French A. S. Localization of fibrinolytic activity in tissues. Brit. med. Bull. 20: 210 1964;
    CrossrefPubMedSearch in Google Scholar
  • 28 French E, Kopec M, Wegrzynowicz Z, Hurwic M, Budzynski A. Z. Influence of fibrinogen degradation products (FDP) on platelet aggregation, adhesiveness and viscous metamorphosis. Thrombos. Diathes. haemorrh. (Stuttg.) 10: 406 1964;
    PubMedSearch in Google Scholar
  • 29 Jerushalmy Z, Zucker M. B. Some effects of fibrinogen degradation products (FDP) on blood platelets. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 413 1965;
    PubMedSearch in Google Scholar
  • 30 Larrieu M. J, Inceman S, Marder V. Action of fibrinogen degradation products on platelet function. Nouv. Rev. franç. Hémat. 7: 691 1967;
    PubMedSearch in Google Scholar
  • 31 Lee L. Reticuloendothelial clearance of circulating fibrin in the pathogenesis of the generalized Shwartzman reaction. J. exp. Med. 115: 1065 1962;
    CrossrefPubMedSearch in Google Scholar
  • 32 Koenig A, Wulf M, Sorg R, Celander E, Celander D. R. Effect of aminomethylcyclohex-ane-carboxylic acid (AMCHA) on the reticuloendothelial system. Fed. Proc. 26: 759 1967;
    PubMedSearch in Google Scholar
  • 33 Hirsh J, Buchanan M, Glynn M. F, Mustard J. F. Effect of activation of the fibrinolytic mechanism on experimental platelet-rich thrombi in rabbits. J. Lab. clin. Med. 72: 245 1968;
    PubMedSearch in Google Scholar
  • 34 Hirsh J, Buchanan M, Glynn M. F, Mustard J. F. Effect of streptokinase on haemostasis. Blood 52: 726 1968;
    PubMedSearch in Google Scholar
  • 35 Kopec M, Wegrzynowicz Z, Budzynski A. A, Latallo Z. S, Lipinski B, Kowalski E. Interaction of fibrinogen degradation products (FDP) with platelets. Exp. Biol. Med. 3: 73 1968;
    PubMedSearch in Google Scholar
  • 36 Sherry S. Perspectives: Fibrinolysis and thrombolysis, Transactions of the conference on haemostasis and thrombosis. Thrombos. Diathes. haemorrh. (Stuttg.). 20 1966
    PubMedSearch in Google Scholar
  • 37 Gessler A. E, McCarty R. S, Parkinson M. G, Bardet J. M. A new and rapid method for isolating viruses by selective fluorocarbon deproteinization. Exp. med. Surg. 7: 4 1949;
    PubMedSearch in Google Scholar