Spontaneous Atrial Thrombosis in Aged Syrian Hamsters II. Hemostasis

W. Jean Dodds; Sharon L. Raymond; Ann C. Moynihan; Donald N. McMartin

doi:10.1055/s-0038-1651851

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 38(02): 0457-0464
DOI: 10.1055/s-0038-1651851

Original Article

Schattauer GmbH

Spontaneous Atrial Thrombosis in Aged Syrian Hamsters II. Hemostasis

W. Jean Dodds

¹Division of Laboratories and Research, New York State Department of Health, Albany, NY 12201, U.S.A. Reprint requests should be directed to the author(s).

,

Sharon L. Raymond

¹Division of Laboratories and Research, New York State Department of Health, Albany, NY 12201, U.S.A. Reprint requests should be directed to the author(s).

,

Ann C. Moynihan

¹Division of Laboratories and Research, New York State Department of Health, Albany, NY 12201, U.S.A. Reprint requests should be directed to the author(s).

,

Donald N. McMartin

¹Division of Laboratories and Research, New York State Department of Health, Albany, NY 12201, U.S.A. Reprint requests should be directed to the author(s).

› Author Affiliations

Abstract

Summary

Coagulation and fibrinolysis were studied in a colony of aged Syrian hamsters with spontaneous atrial thrombosis, and the results are consistent with concomitant consumption coagulopathy. In comparison to age- and sex-matched hamsters from the same colony, those with atrial thrombi had significantly prolonged prothrombin and partial thromboplastin times, reduced levels of factors II, VII, VIII and X activities and plasminogen; and concentrations of fibrinogen-fibrin split products in excess of 80 μg/ml. Hematocrits of the thrombosed animals were significantly decreased, total plasma proteins were increased, leukocyte counts were within normal limits, and platelet counts were about half those of the controls. Thrombosed hamsters had significantly reduced plasma albumin content, increased γ₁-, β-, and γ-globulins, and reduced A/G ratios.

Aged sick hamsters without demonstrable thrombi also had reduced coagulation and fibrinolytic activities and platelet counts, but their fibrinogen levels were markedly elevated, and fibrinogen-fibrin split products were either absent or present in trace amounts. This suggests an earlier and/or less acute form of the thrombotic process.

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References

References
1 Bernhardt B, Valletta M, Brook J, Lejnieks I. 1972; Case report. Amyloidosis with factor X deficiency. American Journal of The Medical Sciences 264: 411.
2 Bowie E. J. W, Thompson Jr. J. H, Didisheim P, Owen C. A. 1971. Mayo Clinic Laboratory Manual of Hemostasis. W. B. Saunders; Philadelphia.:
3 Cooper H. A, Bowie E. J. W, Owen Jr. C. A. 1973; Chronic induced intravascular coagulation in dogs. American Journal of Physiology 225: 1355.
4 Dodds W. J, Pickering R. J. 1972; The effect of cobra venom factor on hemostasis in guinea pigs. Blood 40: 400.
5 Dodds W. J, Raymond S. L, McMartin D. N. 1975; a Atrial thrombosis and consumption coagulopathy in aged Syrian hamsters. Federation Proceedings 34: 221.
6 Dodds W. J, Moynihan A. C, Benson R. E, Hall C. A. 1975; b The value of age- and sex-matched controls for coagulation studies. British Journal of Haematology 29: 305.
7 Galbraith P. A, Sharma N, Parker W. L, Kilgour J. M. 1974; Acquired factor X deficiency. Altered plasma antithrombin activity and association with amyloidosis. Journal of American Medical Association 230: 1658.
8 Johnson A. J, Kline D. L, Alkjaersig N. 1969; Assay methods and standard preparations for plasmin, plasminogen, and urokinase in purified systems. Thrombosis et Diathesis Haemorrhagica 21: 259.
9 McMartin D. N. 1977; Spontaneous atrial thrombosis in aged Syrian hamsters. I. Incidence and pathology. Thrombosis and Haemostasis 38: 447.
10 Merskey C. 1973; Defibrination syndrome or...?. Blood 41: 599.
11 Merskey C, Johnson A. J, Kleiner G. J, Wohl H. 1967; The defibrination syndrome: Clinical features and laboratory diagnosis. British Journal of Haematology 13: 528.
12 Merskey C, Lalezari P, Johnson A. J. 1969; A rapid, simple sensitive method for measuring fibrinolytic split products in human serum. Proceedings of the Society for Experimental Biology and Medicine 131: 871.
13 Ratnoff O. D, Menzie C. 1951; A new method for the determination of fibrinogen in small samples of plasma. Journal of Laboratory and Clinical Medicine 37: 316.
14 Raymond S. L, Dodds W. J. 1975; Characterization of the fawn-hooded rat as a model for hemostatic studies. Thrombosis et Diathesis Haemorrhagica 33: 361.
15 Schalm O. W, Jain N. C, Carroll E. J. 1975. Veterinary Hematology. 3rd edition. Lea & Febiger; Philadelphia.:
16 Selroos O, Pettersson T. 1973; Platelet values in amyloidosis. Scandinavian Journal of Rheumatology 2: 97.
17 Sichuk G, Bettigole R. E, Der B. K, Fortner J. G. 1965; Influence of sex hormones on thrombosis of left atrium in Syrian (golden) hamsters. American Journal of Physiology 208: 465.
18 Van Dam F. E, De Pauw B. E, Schultjes C. P. M, Veuger A. J. L. M. 1975; Isolated factor X deficiency in amyloidosis. Case report and review of the literature. Netherlands Journal of Medicine 18: 129.