Isolation of Antithrombin III from Normal and α1-Antitrypsin-Deficient Human Plasma

Anna D. Borsodi; Ralph A. Bradshaw

doi:10.1055/s-0038-1651853

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 38(02): 0475-0485
DOI: 10.1055/s-0038-1651853

Original Article

Schattauer GmbH

Isolation of Antithrombin III from Normal and α₁-Antitrypsin-Deficient Human Plasma

Anna D. Borsodi^*

¹Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110, U. S. A.

,

Ralph A. Bradshaw

¹Department of Biological Chemistry, Washington University School of Medicine, St. Louis, MO 63110, U. S. A.

› Author Affiliations

Abstract

Summary

The plasma of individuals, hetero- or homozygous for α₁-antitrypsin deficiency, contains greatly decreased amounts of antithrombin activity as assayed against factor Xa. However, heparin stimulation of the residual antithrombin activity is observed, which is comparable to that of normal plasma. Antithrombins isolated from both normal and α₁-antitrypsin deficient plasma by a simplified procedure are indistinguishable in both properties and yields. The microheterogeneity observed on isoelectric focusing of both preparations can be eliminated by treatment with neuraminidase. Neither purified human antithrombin nor α₁-antitrypsin, when assayed against bovine trypsin, is stimulated by heparin. These results clearly establish the unique natures of antithrombin and α₁-antitrypsin and show that about 75% of the antithrombin activity measured in normal plasma is due to α₁-antitrypsin. Estimates of anti thrombin III activity in normal plasma by assays dependent on enzymatic activity can probably be obtained only in the presence of heparin.

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References

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