Exterior and Interior Events in Early Stage of Thrombin-induced Platelet Aggregation

Shuichi Hashimoto; Sachiko Shibata; Bonro Kobayashi

doi:10.1055/s-0038-1651878

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1977; 38(03): 0630-0639
DOI: 10.1055/s-0038-1651878

Original Article

Schattauer GmbH

Exterior and Interior Events in Early Stage of Thrombin-induced Platelet Aggregation

Authors

Shuichi Hashimoto

¹Radioisotope Research Laboratory and Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan
Sachiko Shibata

¹Radioisotope Research Laboratory and Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan
Bonro Kobayashi

¹Radioisotope Research Laboratory and Department of Physiological Chemistry, School of Pharmaceutical Sciences, Kitasato University, Tokyo 108, Japan

Abstract

Summary

Treatment of washed rabbit platelets with 1 u/ml of thrombin at 37° C resulted in a disappearance from platelets of a protein with 250,000 dalton molecular weight which was shown to be originated from plasma membrane. Parallel loss of adenyl cyclase was noted, and both reactions were complete within 30 sec. From the patterns of disc electrophoretograms, the importance of quick suppression of thrombin action in demonstrating the primary event was stressed.

Thrombin induced an apparent activation of membrane bound phosphodiesterase. This reaction was also complete within 30 sec. The cellular component which contained the enzyme activity was distinct from plasma membrane. Soluble phosphodiesterase was not influenced by thrombin at all.

These reactions required intact platelet cells to react with thrombin, and no reaction was detected when subcellular preparations were treated with thrombin.

Possibility of collaboration of changes in externally located synthetic enzyme with those in internally located degrading enzyme in the early phase of thrombin action on platelets was suggested.

Full Text

References

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