Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1969; 21(02): 259-272DOI: 10.1055/s-0038-1653535 Originalarbeiten - Original Articles - Travaux Originaux Schattauer GmbH Assay Methods and Standard Preparations for Plasmin, Plasminogen and Urokinase in Purified Systems, 1967-1968[*] Authors Author Affiliations A. J Johnson (Chairman) 1 Recommendations of the N. H. I. Committee on Thrombolytic Agents, Subcommittee for Standardization D. L Kline 1 Recommendations of the N. H. I. Committee on Thrombolytic Agents, Subcommittee for Standardization Norma Alkjaersig 1 Recommendations of the N. H. I. Committee on Thrombolytic Agents, Subcommittee for Standardization Recommend Article Abstract Buy Article(opens in new window) Summary To facilitate communication between investigators, the Committee on Thrombolytic Agents of the National Heart Institute suggests standardized reagents and assay methods for the measurement of standard preparations of plasmin, plasminogen, and urokinase with use of casein, fibrin and synthetic esters as substrates. Full Text References References 1 Enzyme Nomenclature. Elsevier Publishing Co; Amsterdam, London, New York: 1965: 7. 2 Johnson A. J, Tse A, Skoza L. Variation in assays of standard SK and plasmin preparations : evidence for and effect of variability in plasmins, plasminogen, and fibrinogen. Fed. Proc 25: 34 1966; 3 Walton P. L. The hydrolysis of α-N-acetylglycyl-L-lysine methyl ester by urokinase. Biochim. biophys. Acta (Amst) 132: 104 1967; 4 Hipp N. J, Groves M. L, Custer J. H, McMeekin T. L. Separation of alpha, beta and gamma casein. J. Dairy Science 35: 272 1952; 5 Kline D. L, Fishman J. B. Improved procedure for the isolation of human plasminogen. J. biol. Chem 236: 3232 1961; 6 Johnson A. J, Bergström K, Tse A. O. A simple procedure for the preparation of highly purified soluble human plasminogen. (Abstract) Fed. Proc 26: 647 1967; 7 Remmert L. F, Cohen P. P. Partial purification and properties of a proteolytic enzyme of human serum. J. biol. Chem 181: 431 1949; 8 Sgouris J. T, Inman J. K, McCall K. B, Hyndman L. A, Anderson H. D. The preparation of human fibrinolysin (plasmin). Vox Sang. (Basel) 05: 357 1960; 9 Christensen L. R. The action of proteolytic enzymes on casein proteins. Arch. Biochem 53: 128 1954; 10 Alkjaersig N, Fletcher A. P, Sherry S. e-aminocaproic acid: an inhibitor of plasminogen activation. J. biol. Chem 234: 832 1959; 11 Derechin M. The assay of human plasminogen with casein as substrate. Biochem. J 78: 443 1961; 12 Johnson A. J, McCarty W. R, Tillett W. S, Tse A. O, Skoza L, Newman J, Semar M. Fibrinolytic, Caseinolytic and Biochemical Methods for the Study of Thrombolysis in Man : Application and Standardization. In: Blood Coagulation, Hemorrhage and Thrombosis. Tocantins L. M, Kazal L. A. Eds. Grune & Stratton; New York: 1964: 449-503. 13 Gliffton E. E, Downie G. R. Variations in proteolytic activity of serum of animals including man. Proc. Soc. exp. Biol. (N. Y) 73: 559 1950; 14 Gelander D. R, Guest M. M. The biochemistry and physiology of urokinase. Amer. J. Cardiol 06: 409 1960; 15 Kjeldgaard N. O, Ploug J. Urokinase, an activator of plasminogen from human urine. II. Mechanism of plasminogen activation. Biochim. biophys. Acta (Amst) 24: 283 1957; 16 Alkjaersig N, Fletcher A. P, Sherry S. The activation of human plasminogen: a kinetic study of activation with trypsin, urokinase, and streptokinase. J. biol. Chem 233: 86 1958; 17 Sherry S, Alkjaersig N, Fletcher A. P. Assay of urokinase preparations with the synthetic substrate acetyl-L-lysine methyl ester. J. Lab. clin. Med 64: 145 1964; 18 Siegelman A. M, Carlsen A. S, Robertsen T. Investigation of serum trypsin and related substances. I. The quantitative determination of trypsin-like activity in human blood serum by a micromethod. Arch. Biochem 97: 159 1962; 19 Milstone H. A. A factor in normal human blood which participates in streptococcal fibrinolysis. J. Immunol 42: 109 1941; 20 Johnson A. J. Unpublished data. 21 Sherry S, Lindemeyer R. I, Fletcher A. P, Alkjaersig N. Studies on enhanced fibrinolytic activity in man. J. clin. Invest 38: 810 1959; 22 Lesuk A, Terminiello L, Traver J. H, Groff J. L. Proteolytic degradation of human urokinase to active fragments. (Abstract) Fed. Proc 26: 647 1967; 23 Korsgaard-Ghristensen L. A method for determination of trypsin inhibitors in urine and serum. Scand. J. clin. Lab. Invest 10: 177 1958; 24 Faarvang H. J. The excretion of trypsin inhibitor in urine in normal persons. Acta endocr. (Kbh) 30: 285 1959; 25 Astrup T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem 40: 346 1952; 26 Bjerrehuus I. Fibrinolytic activity of urine. Scand. J. clin. Lab. Invest 04: 179 1952; 27 Smyrniotis F. E, Fletcher A. P, Alkjaersig N, Sherry S. Urokinase excretion in health and its alteration in certain disease states. Thrombos. Diathes. haemorrh. (Stuttg) 03: 257 1959;
