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Thromb Haemost 1970; 24(01/02): 152-160
DOI: 10.1055/s-0038-1654220
Originalarbeiten – Original Articles – Travaux Originaux
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Corrective Effect of Sialic Acid on the Clotting of Factor VIII Deficient Blood

H Rubin
1   Division of Hematology, Department of Medicine, Maimonides Medical Center, Brooklyn, New York, and the State University of New York, Downstate Medical Center, Brooklyn, New York
,
T Fernbach
1   Division of Hematology, Department of Medicine, Maimonides Medical Center, Brooklyn, New York, and the State University of New York, Downstate Medical Center, Brooklyn, New York
,
N. D Ritz
1   Division of Hematology, Department of Medicine, Maimonides Medical Center, Brooklyn, New York, and the State University of New York, Downstate Medical Center, Brooklyn, New York
› Author AffiliationsThese studies were supported by grants from the Metropolitan Chapter of the National Hemophilia Foundation and Brooklyn Hemophilia Guild.
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Publication Date:
28 June 2018 (online)

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Summary

Optimal concentrations of N-acetyl neuramic acid (NANA) accelerated the clotting of factor VIII deficient whole blood and plasma. There was no effect on normal plasma and certain other factor deficient plasmas.

NANA increased thromboplastin formation by factor VIII deficient plasma, as measured by the Thromboplastin Generation Test.

NANA possesses an inhibitory effect on rabbit brain thromboplastin and cephalin in recalcification tests, over the values of its controls, but-in sharp contrast, enhanced the effect of Russell Viper Venom (RVV) and RVV-cephalin.

The amino sugar N-acetyl mannosamine, possessing common functional groups with NANA, also shortened the clotting time of factor VIII deficient whole blood and plasma.

It is suggested that NANA acts as a cofactor to factor VIII which is present in trace amounts in hemophilic plasma.

 

  • References

  • 1 Rubin H, Ritz N. D. The inhibitory effect of sialic acid on fibrinolysis. Thrombos. Diathes. haemorrh. (Stuttg) 17: 23-30 1967;
    PubMedSearch in Google Scholar
  • 2 Pool J. G, Robinson J. Observations on Plasma banking and transfusion procedure for haemophilic patients using a quantitative assay for anti-haemophilic globulin (AHG). Brit. J. Haem 05: 24 1959;
    CrossrefPubMedSearch in Google Scholar
  • 3 Biggs R, Douglas A. S. The thromboplastin generation test. J. clin. Path 06: 23 1953;
    CrossrefPubMedSearch in Google Scholar
  • 4 Proctor R. R, Rapaport S. I. The partial thromboplastin time with Kaolin. Amer. J. clin. Path 36: 212 1961;
    PubMedSearch in Google Scholar
  • 5 Borchgrevink C. F, Pool J. G, Storkmorken H. A new assay for Factor V (Proaccelerin Accelerin). J. Lab. clin. Med 55: 625 1960;
    PubMedSearch in Google Scholar
  • 6 Bachmann F, Duckert F, Koller F. The Stuart-Prower factor assay and its clinical significance. Thrombos. Diathes. haemorrh. (Stuttg) 02: 24 1958;
    PubMedSearch in Google Scholar
  • 7 Rapaport S. I, Schiffman S, Patch M. J, Ware A. G. A simple specific one stage assay far plasma thromboplastin antecedent activity. J. Lab. clin. Med 57: 771 1961;
    PubMedSearch in Google Scholar
  • 8 Marchal G, Leroux M. E, Samama M. Atlas de thrombodynamagraphie. Paris, Editeur Sevice de Propaganda. Edition, Information.
    PubMed
  • 9 Langdell R. D. Partial thromboplastin time method for determination of antihemophilic factor. 11-14 In: The Hemophilias. International Symposium. Ed. Kenneth M. Brinkhouse University of Nort Carolina Press; Washington, D. C., Chapel Hill, N. C: 1964
    Search in Google Scholar
  • 10 Fullerton W. W, Boggust W. A, O’Meara R. A. Q. Antithromboplastic and thromboplastic activities of fatty acids. J. clin. Path 20: 624-628 1967;
    CrossrefPubMedSearch in Google Scholar
  • 11 Cohen P, Cooley M. H, Gardner F. H. Platelet preservation. III. Comparison of radioactivity fields of platelet concentrates derived from blood anticoagulated with EDTA and ACD. N. Engl. J. Med 273: 845 1965;
    CrossrefPubMedSearch in Google Scholar
  • 12 Gilchrist G. S, Ekert H. Reduction of Factor VIII activity in cryoprecipitate obtained from acidified plasma. Transfusion (Philad) 08: 294-298 1968;
    CrossrefPubMedSearch in Google Scholar
  • 13 Graham J. B, Barrow E. M. The pathogenesis of hemophilia: An experimental analysis of the anticephalin hypothesis in hemophilic dogs. J. exp. Med 106: 273-292 1957;
    CrossrefPubMedSearch in Google Scholar
  • 14 Hemker H. C, Kahn J. P. Reaction sequence of blood coagulation. Nature (Lond) 215: 1201 1967;
    CrossrefPubMedSearch in Google Scholar
  • 15 Barton P. G. Sequence theories of blood coagulation re-evaluated with reference to lipid protein interactions. Nature (Lond) 215: 1508 1967;
    CrossrefPubMedSearch in Google Scholar
  • 16 Esnouf M. P. Biochemical aspects of blood coagulation. In plenary session papers XII Congress International Society of Hematology. N. Y: 1968: 315-318.
    Search in Google Scholar