Corrective Effect of Sialic Acid on the Clotting of Factor VIII Deficient Blood

H Rubin; T Fernbach; N. D Ritz

doi:10.1055/s-0038-1654220

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1970; 24(01/02): 152-160
DOI: 10.1055/s-0038-1654220

Originalarbeiten – Original Articles – Travaux Originaux

Schattauer GmbH

Corrective Effect of Sialic Acid on the Clotting of Factor VIII Deficient Blood

Authors

H Rubin

¹Division of Hematology, Department of Medicine, Maimonides Medical Center, Brooklyn, New York, and the State University of New York, Downstate Medical Center, Brooklyn, New York
T Fernbach

¹Division of Hematology, Department of Medicine, Maimonides Medical Center, Brooklyn, New York, and the State University of New York, Downstate Medical Center, Brooklyn, New York
N. D Ritz

¹Division of Hematology, Department of Medicine, Maimonides Medical Center, Brooklyn, New York, and the State University of New York, Downstate Medical Center, Brooklyn, New York

Abstract

Summary

Optimal concentrations of N-acetyl neuramic acid (NANA) accelerated the clotting of factor VIII deficient whole blood and plasma. There was no effect on normal plasma and certain other factor deficient plasmas.

NANA increased thromboplastin formation by factor VIII deficient plasma, as measured by the Thromboplastin Generation Test.

NANA possesses an inhibitory effect on rabbit brain thromboplastin and cephalin in recalcification tests, over the values of its controls, but-in sharp contrast, enhanced the effect of Russell Viper Venom (RVV) and RVV-cephalin.

The amino sugar N-acetyl mannosamine, possessing common functional groups with NANA, also shortened the clotting time of factor VIII deficient whole blood and plasma.

It is suggested that NANA acts as a cofactor to factor VIII which is present in trace amounts in hemophilic plasma.

Full Text

References

References
1 Rubin H, Ritz N. D. The inhibitory effect of sialic acid on fibrinolysis. Thrombos. Diathes. haemorrh. (Stuttg) 17: 23-30 1967;
2 Pool J. G, Robinson J. Observations on Plasma banking and transfusion procedure for haemophilic patients using a quantitative assay for anti-haemophilic globulin (AHG). Brit. J. Haem 05: 24 1959;
3 Biggs R, Douglas A. S. The thromboplastin generation test. J. clin. Path 06: 23 1953;
4 Proctor R. R, Rapaport S. I. The partial thromboplastin time with Kaolin. Amer. J. clin. Path 36: 212 1961;
5 Borchgrevink C. F, Pool J. G, Storkmorken H. A new assay for Factor V (Proaccelerin Accelerin). J. Lab. clin. Med 55: 625 1960;
6 Bachmann F, Duckert F, Koller F. The Stuart-Prower factor assay and its clinical significance. Thrombos. Diathes. haemorrh. (Stuttg) 02: 24 1958;
7 Rapaport S. I, Schiffman S, Patch M. J, Ware A. G. A simple specific one stage assay far plasma thromboplastin antecedent activity. J. Lab. clin. Med 57: 771 1961;
8 Marchal G, Leroux M. E, Samama M. Atlas de thrombodynamagraphie. Paris, Editeur Sevice de Propaganda. Edition, Information.
9 Langdell R. D. Partial thromboplastin time method for determination of antihemophilic factor. 11-14 In: The Hemophilias. International Symposium. Ed. Kenneth M. Brinkhouse University of Nort Carolina Press; Washington, D. C., Chapel Hill, N. C: 1964
10 Fullerton W. W, Boggust W. A, O’Meara R. A. Q. Antithromboplastic and thromboplastic activities of fatty acids. J. clin. Path 20: 624-628 1967;
11 Cohen P, Cooley M. H, Gardner F. H. Platelet preservation. III. Comparison of radioactivity fields of platelet concentrates derived from blood anticoagulated with EDTA and ACD. N. Engl. J. Med 273: 845 1965;
12 Gilchrist G. S, Ekert H. Reduction of Factor VIII activity in cryoprecipitate obtained from acidified plasma. Transfusion (Philad) 08: 294-298 1968;
13 Graham J. B, Barrow E. M. The pathogenesis of hemophilia: An experimental analysis of the anticephalin hypothesis in hemophilic dogs. J. exp. Med 106: 273-292 1957;
14 Hemker H. C, Kahn J. P. Reaction sequence of blood coagulation. Nature (Lond) 215: 1201 1967;
15 Barton P. G. Sequence theories of blood coagulation re-evaluated with reference to lipid protein interactions. Nature (Lond) 215: 1508 1967;
16 Esnouf M. P. Biochemical aspects of blood coagulation. In plenary session papers XII Congress International Society of Hematology. N. Y: 1968: 315-318.