Download PDF
Thromb Haemost 1961; 6(01): 001-014
DOI: 10.1055/s-0038-1654534
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Tissue Extract and the Contact Reaction in Blood Coagulation

Rosemary Biggs
1   The Blood Coagulation Research Unit, Churchill Hospital, Oxford
,
H. L Nossel   *
1   The Blood Coagulation Research Unit, Churchill Hospital, Oxford
› Author Affiliations
Further Information

Publication History

Publication Date:
12 July 2018 (online)

Also available at
    Permissions and Reprints

Summary

1. The addition of dilute brain extract to normal silicone collected plasma or to celite exhausted normal plasma produces an acceleration of thrombin formation similar to that of kaolin on normal plasma.

2. When dilute brain extract is added to PTA or Hageman deficient plasma samples, the concentration of brain required to simulate the kaolin activated normal contact mechanism is similar to that required for normal plasma. When very dilute brain extract is used the reaction of the Hageman deficient sample is defective.

3. When added to factor VIII or factor IX deficient plasma samples about ten times as much brain extract is required to produce the type of thrombin generation curve typically found on the addition of kaolin to silicone collected normal plasma.

4. It is suggested that in normal haemostasis the contact phase may be initiated, in part at least, by weak tissue extracts liberated at a site of injury.

* Research Bursar, South African Council for Scientific and Industrial Research.


 

  • References

  • (1) Alexander B. Discussion on the hemophiloid states. In: Hemophilia and Hemophilioid Diseases. Brinkhous K. M. Editor 131 University of North Carolina Press; 1957
    Search in Google Scholar
  • (2) Biggs R, Macfarlane R. G. The reaction of haemophilic plasma to thromboplastin. J. clin. Path. 4: 445 1951;
    CrossrefPubMedSearch in Google Scholar
  • (3) Biggs R, Macfarlane R. G. Human Blood Coagulation and Its Disorders. Blackwell Scientific Publications; Oxford: 1957
    Search in Google Scholar
  • (4) Biggs R, Sharp A. A, Margolis J, Hardisty R. M, Stewart J, Davidson W. M. Defects in the early stages of blood coagulation: A report of four cases. Brit. J. Haemat. 4: 177 1958;
    CrossrefPubMedSearch in Google Scholar
  • (5) Egeberg O. Assay of antihaemophilic A, B and C factors by one-stage cephalin systems. Scand. J. clin. Lab. Invest. 13: 140 1961;
    CrossrefPubMedSearch in Google Scholar
  • (6) Haanen C, Hommes F, Benraad H, Morselt G. A case of Hageman-factor deficiency and a method to purify the factor. Thromb. Diath. haem. 5: 201 1960;
    PubMedSearch in Google Scholar
  • (7) Hardisty R. M. The reaction of blood coagulation factors with brain extract. Brit. J. Haemat. 1: 323 1955;
    CrossrefPubMedSearch in Google Scholar
  • (8) Margolis J. The kaolin clotting time. J. clin. Path. 11: 406 1958;
    CrossrefPubMedSearch in Google Scholar
  • (9) Owren P. A. The present state of the converting and accelerator factors in prothrombin conversion. In: Report of the International Conference on Thrombosis and Embolism. 65 Schwabe; Basel: 1954
    Search in Google Scholar
  • (10) Owren P. A, Aas K. The control of dicoumarol therapy and the quantitative determination of prothrombin and proconvertin. Scand. J. clin. Lab. Invest. 3: 201 1951;
    CrossrefPubMedSearch in Google Scholar
  • (11) Pavlovsky A, Mittelman D, Castellanos H. Consuncion de protrombina. Rev. Soc. argent. Hernat. 1: 305 1949;
    PubMedSearch in Google Scholar
  • (12) Ratnoff O. D, Margolius A. Hageman factor and Hageman trait. In: Hemophilia and Hemophilioid Diseases. Brinkhous K. M. Editor 124 University of North Carolina Press; 1957
    Search in Google Scholar
  • (13) Rosenthal R. L. The present status of plasma thromboplastin antecedent (PTA) deficiency. In: Hemophilia and Hemophilioid Diseases. Brinkhous K. M. Editor 116 University of North Carolina Press; 1957
    Search in Google Scholar
  • (14) Soulier J.-P, Prou-Wartelle O. New data on Hageman factor and plasma thromboplastin antecedent: the role of “contact” in the initial phase of blood coagulation. Brit. J. Haemat. 6: 88 1960;
    CrossrefPubMedSearch in Google Scholar
  • (15) Waaler B. A. Simultaneous contribution to the formation of thrombin by the intrinsic and extrinsic blood clotting systems. Scand. J. clin. Lab. Invest. 9: 322 1957;
    CrossrefPubMedSearch in Google Scholar
  • (16) Waaler B. A. Contact activation in the intrinsic blood clotting system. Scand. J. clin. Lab. Invest.. 11. Suppl. 37 1959
    PubMedSearch in Google Scholar