The Formation of Intermediate Product I in a Purified System The Role of Factor IX or of its Precursor and of a Hageman Factor-PTA Fraction

Summary

1. The role of two clot promoting fractions isolated from either plasma or serum is studied in a purified system for the generation of intermediate product I in which the serum is replaced by factor X and the investigated fractions.

2. Optimal generation of intermediate product I is possible in the purified system utilizing fractions devoid of factor IX one-stage activity. Prothrombin and thrombin are not necessary in this system.

3. The fraction containing factor IX or its precursor, no measurable activity by the one-stage assay method, controls the yield of intermediate product I. No similar fraction can be isolated from haemophilia B plasma or serum.

4. The Hageman factor — PTA fraction shortens the lag phase of intermediate product I formation and has no influence on the yield. This fraction can also be prepared from haemophilia B plasma or serum.

• References

• (1) Bachmann F, Duckert F, Fisch U, Streuli F, Gerber D, Koller F. Der Gerinnungsdefekt beim kongenitalen PTA-Mangel. Schweiz. med. Wschr. 88: 1037 1958;
• (2) Bachmann F, Duckert E, Koller F. The Stuart-Prower Factor Assay and Its Clinical Significance. Thromb. Diath. haem. 2: 24 1958;
• (3) Duckert F, Flückiger P, Isenschmid H, Matter M, Vogel-Meng J, Koller F. A Modification of the Thromboplastin Generation Test. Acta haemat. (Basel) 12: 197 1954;
• (4) Duckert F, Flückiger P, Matter M, Koller F. Clotting Factor X. Physiologic and Physico-Chemical Properties. Proc. Soc. exp. Biol. (N. Y.) 90: 17 1955;
• (5) Duckert F, Loeligeret A, Koller F. Sur un nouveau Facteur de la Coagulation, le Facteur VII. Sa Purification. Helv. chim. Acta 34: 2431 1951;
• (6) Duckert F, Yin E. T, Straub W. Separation and Purification of the Blood Clotting Factors by means of Chromatography and Electrophoresis. Preparative methods. Protides of the Biological Fluids. Proc. 8th. Colloquium, Bruges 1960. 41 Elsevier; Amsterdam: 1961
• (7) Fisch U, Duckert F. Some Aspects of Kinetics of the First Stages of Thromboplastin Formation. Thromb. Diath. haem. 3: 98 1959;
• (8) Geiger M, Duckert F, Koller F. Quantitative Bestimmungen von Faktor VIII und IX bei Blutersippen. 413 5th Cong. Europ. Soc. Haemat. Freiburg i. B. 1956. Springer; Heidelberg: 1956
• (9) Koller F, Loeliger A, Duckert F. Experiments on a New Clotting Factor VII. Acta haemat. (Basel) 6: 1 1951;
• (10) Yin E. T, Duckert F. Purification of Factor IX or of Its Precursor. Isolation of a Hageman-PTA Fraction. Thromb. Diath. haem. 6: 215 1961;