The Formation of Intermediate Product I in a Purified System The Role of Factor IX or of its Precursor and of a Hageman Factor-PTA Fraction

E. T Yin; F Duckert

doi:10.1055/s-0038-1654555

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1961; 6(02): 224-234
DOI: 10.1055/s-0038-1654555

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

The Formation of Intermediate Product I in a Purified System The Role of Factor IX or of its Precursor and of a Hageman Factor-PTA Fraction

Authors

E. T Yin

¹Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) Kantonsspital, University of Zürich, Switzerland
F Duckert

¹Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) Kantonsspital, University of Zürich, Switzerland

Abstract

Summary

1. The role of two clot promoting fractions isolated from either plasma or serum is studied in a purified system for the generation of intermediate product I in which the serum is replaced by factor X and the investigated fractions.

2. Optimal generation of intermediate product I is possible in the purified system utilizing fractions devoid of factor IX one-stage activity. Prothrombin and thrombin are not necessary in this system.

3. The fraction containing factor IX or its precursor, no measurable activity by the one-stage assay method, controls the yield of intermediate product I. No similar fraction can be isolated from haemophilia B plasma or serum.

4. The Hageman factor — PTA fraction shortens the lag phase of intermediate product I formation and has no influence on the yield. This fraction can also be prepared from haemophilia B plasma or serum.

Full Text

References

References
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