Purification of Antihemophilic Factor (Factor VIII) by Amino Acid Precipitation*

Summary

1. The use of several amino acids, glycine, alpha-aminobutyric acid, alanine, beta-alanine, and gamma-aminobutyric acid, as plasma protein precipitants is described.

2. A specific procedure is detailed for the preparation of canine antihemophilic factor (AHF, Factor VIII) in which glycine, beta-alanine, and gammaaminobutyric acid serve as the protein precipitants.

3. Preliminary results are reported for the precipitation of bovine and human AHF with amino acids.

^** This investigator was supported by a Public Health Service research career program award (K3-GM-1147).

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