Summary
The plasma protein, fibrinogen, has been prepared in a relatively pure form in this
laboratory. Utilizing paper electrophoresis this protein has been studied. Under normal
conditions fibrinogen does not show migration from the origin; however, in the presence
of the non-ionic detergent, Triton X-100, migration is noted at several pH values.
The clotted product, fibrin, does not demonstrate migration from the origin under
any condition. Several intermediates of the clotting sequence were also studied by
paper electrophoresis in the presence and absence of the detergent and urea at several
pH values. The results obtained are discussed with regard to the current hypothesis
of clotting, and the products present under various conditions.
