Download PDF
Thromb Haemost 1967; 18(03/04): 537-543
DOI: 10.1055/s-0038-1655063
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

Comparative Effects of ADP and Thrombin in Producing Stasis Thrombosis

R. K Stuart
1   Vascular Laboratory, Lemuel Shattuck Hospital and Department of Medicine, Tufts University School of Medicine,Boston, Massachusetts, U.S.A.
,
D. P Thomas
1   Vascular Laboratory, Lemuel Shattuck Hospital and Department of Medicine, Tufts University School of Medicine,Boston, Massachusetts, U.S.A.
› Author AffiliationsThus study was supported by research grants HE-09203-02 and HE 5616 from the National Heart Institute, National Institutes of Health.
We wish to thank Dr. Hugo Muench and Miss Rasma Klints for the statistical analysis.
Further Information

Publication History

Publication Date:
26 June 2018 (online)

Also available at
    Permissions and Reprints

Summary

1. The effect of intravenous ADP on the platelet count, whole blood clotting time and stasis thrombosis formation was investigated in rabbits.

2. 20 μ/kg and 10 mg/kg of ADP produced, respectively, a moderate and marked reduction in the circulating platelet count.

3. Both the low and high doses of ADP resulted in only a relatively slight shortening of the whole blood clotting time.

4. Neither dose of ADP resulted in thrombus formation in isolated venous segments after 30 min of stasis.

5. It is concluded that ADP alone is unlikely to be an initiating factor in the pathogenesis of stasis thrombosis.

 

  • References

  • 1 Hellem A. J. The adhesiveness of human blood platelets in vitro. Scand. J. clin. Lab. Inv. 12. Suppl. 51 1960
    PubMedSearch in Google Scholar
  • 2 Ollgaard E. Macroscopic studies of platelet aggregation. Nature of an aggregating factor in red blood cells and platelets. Thrombos. Diathes. haemorrh. (Stuttg) 06: 86 1961;
    PubMedSearch in Google Scholar
  • 3 Gaarder A, Johnsen J, Laland S, Hellem A, Owren P. A. Adenosine diphosphate in red cells as a factor on the adhesiveness of human blood platelets. Nature (Lond) 192: 531 1961;
    CrossrefPubMedSearch in Google Scholar
  • 4 Born G VR. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature (Lond) 194: 927 1962;
    PubMedSearch in Google Scholar
  • 5 O’Brien J. R. Platelet aggregation. I. Some effects of adenosine diphosphate, thrombin and cocaine upon platelet adhesiveness. J. clin. Pathol. 15: 446 1962;
    CrossrefPubMedSearch in Google Scholar
  • 6 Born G VR, Cross M. J. Effect of adenosine diphosphate on the concentration of platelets in circulating blood. Nature (Lond) 197: 974 1963;
    CrossrefPubMedSearch in Google Scholar
  • 7 Honour A. J, Mitchell J. R. A. Platelet clumping in vivo. Nature (Lond) 197: 1019 1963;
    PubMedSearch in Google Scholar
  • 8 Davey M. G, Lander H. Effects of adenosine diphosphate on circulating platelets in man. Nature (Lond) 201: 1037 1964;
    PubMedSearch in Google Scholar
  • 9 Käser-Glanzmann R, Lüscher E. F. Mechanism of platelet aggregation in relation to hemostasis. Thrombos. Diathes. haemorrh. (Stuttg) 07: 480 1962;
    PubMedSearch in Google Scholar
  • 10 Honour A. J, Mitchell J. R. A. Platelet clumping in injured vessels. Brit. J. exp. Pathol. 45: 75 1964;
    PubMedSearch in Google Scholar
  • 11 Mustard J. F, Roswell H. G, Lotz F, Hegardt B, Murphy E. A. The effect of adenosine nucleotides on thrombus formation, platelet count and blood coagulation. Exp. mol. Pathol. 05: 43 1966;
    CrossrefPubMedSearch in Google Scholar
  • 12 Zucker M. B, Bonelli J. Platelet clumping produced by connective tissue suspension and by collagen. Proc. Soc. exp. Biol. (N. Y) 109: 779 1962;
    CrossrefPubMedSearch in Google Scholar
  • 13 Hovig T. Release of a platelet aggregating substance (adenosine diphosphate) from rabbit blood platelets induced by saline extract of tendons. Thrombos. Diathes. haemorrh. (Stuttg) 09: 264 1963;
    PubMedSearch in Google Scholar
  • 14 French J. E, MacFarlane R. G, Sanders A. G. The structure of hemostatic plugs and experimental thrombi in small arteries. Brit. J. exp. Pathol. 45: 467 1964;
    PubMedSearch in Google Scholar
  • 15 Marr J, Barboriak J. J, Johnson S. A. Relationship of appearance of adenosine diphosphate, fibrin formation and platelet aggregation in the hemostatic plug in vivo. Nature (Lond) 205: 259 1965;
    CrossrefPubMedSearch in Google Scholar
  • 16 Dintenfass L. Some rheological factors in the pathogenesis of thrombosis. Lancet II: 370 1965;
    PubMedSearch in Google Scholar
  • 17 McLachlin A. D, McLachlin J. A, Jory T. A, Rawlings E. G. Venous stasis in the lower extremities. Ann. Surg. 152: 678 1960;
    CrossrefPubMedSearch in Google Scholar
  • 18 McLachlin J, Patterson J. G. Precipitating factors in venous thrombosis. Surg. Gynec. Obstet. 98: 96 1954;
    PubMedSearch in Google Scholar
  • 19 Sevitt S. Personal communication..
    PubMed
  • 20 Wessler S. Mechanism of stasis thrombi. Pulmonary Embolic Disease. 44 Eds. Sasahara A. A, Stein M. Grune and Stratton; New York: 1965
    Search in Google Scholar
  • 21 Wessler S. Thrombosis in the presence of vascular stasis. Amer. J. Med. 33: 648 1962;
    CrossrefPubMedSearch in Google Scholar
  • 22 Nam S. C, Lee K. T, Boylan J, Thomas A. W. Dietary lipid and thrombosis: study of trigger mechanism for thrombosis in stock fed rats. Exp. mol. Pathol. 04: 232 1965;
    CrossrefPubMedSearch in Google Scholar
  • 23 Arakawa T, Spaet T. H. Hypercoagulability and thrombosis: Effect of injected thrombo- kinase and adenosine diphosphate on established microthrombi in rabbits. Proc. Soc. exp. Biol. (N. Y) 116: 1034 1964;
    CrossrefPubMedSearch in Google Scholar
  • 24 Biggs R, MacFarlane R. G. Human blood coagulation and its disorders. Blackwell Scientific Publications; Oxford: Third Edition. 1962
    Search in Google Scholar
  • 25 Bounameaux Y. Sur le mecahisme de la retraction du caillot et de la metamorphose visqueuse des plaquettes. Rev. Hémat. 12: 16 1957;
    PubMedSearch in Google Scholar
  • 26 Brecher G, Cronkite E. P. Morphology and enumeration of human blood platelets. J. appl. Physiol. 03: 365 1950;
    CrossrefPubMedSearch in Google Scholar
  • 27 Wessler S. Studies in intravascular coagulation. III. The pathogenesis of serum induced venous thrombosis. J. clin. Invest. 34: 647 1955;
    CrossrefPubMedSearch in Google Scholar
  • 28 Botti R. E, Ratnoff O. D. Studies on the pathogenesis of thrombosis: An experimental “Hypercoagulable” state induced by the intravenous injection of ellagic acid. J. Lab. clin. Med. 64: 385 1964;
    PubMedSearch in Google Scholar
  • 29 Gurewich V, Thomas D. P. Pathogenesis of venous thrombosis in relation to its prevention by dextran and heparin. J. Lab. clin. Med. 66: 604 1965;
    PubMedSearch in Google Scholar
  • 30 Nordoy A, Chandler A. B. Platelet thrombosis induced by adenosine diphosphate in the rat. Scand. J. Haemat. 01: 16 1964;
    PubMedSearch in Google Scholar
  • 31 Mustard J. F, Hegardt B, Rowsell H. C, MacMillan R. L. Effect of adenosine nucleotides on platelet aggregation and clotting time. J. Lab. clin. Med. 64: 548 1964;
    PubMedSearch in Google Scholar
  • 32 Pizzuto J, Giorgio A. J, Didisheim P. The clot-promoting effect of adenosine-5-diphosphate (ADP). I. Mechanisms of action. Thrombos. Diathes. haemorrh. (Stuttg) 11: 428 1966;
    PubMedSearch in Google Scholar
  • 33 Hardisty R. M, Hutton R. A. Platelet aggregation and the availability of platelet factor 3, Brit. J. Haemat. 12: 764 1966;
    CrossrefPubMedSearch in Google Scholar