The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis

Thomas J. Stocker; Joachim Pircher; Artid Skenderi; Andreas Ehrlich; Clemens Eberle; Remco T. A. Megens; Tobias Petzold; Zhe Zhang; Barbara Walzog; Annette Müller-Taubenberger; Christian Weber; Steffen Massberg; Hellen Ishikawa-Ankerhold; Christian Schulz

doi:10.1055/s-0038-1675604

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2018; 118(12): 2098-2111
DOI: 10.1055/s-0038-1675604

Cellular Haemostasis and Platelets

Georg Thieme Verlag KG Stuttgart · New York

The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis

Thomas J. Stocker^*

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

²DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

,

Joachim Pircher^*

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

²DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

,

Artid Skenderi

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

,

Andreas Ehrlich

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

,

Clemens Eberle

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

,

Remco T. A. Megens

³Institute for the Prevention of Cardiovascular Diseases (IPEK), Ludwig-Maximilians-Universität München, Munich, Germany

⁴Department of Biomedical Engineering, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands

,

Tobias Petzold

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

²DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

,

Zhe Zhang

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

,

Barbara Walzog

⁵Walter Brendel Centre of Experimental Medicine, Department of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-Universität, Planegg-Martinsried, Germany

,

Annette Müller-Taubenberger

⁶Department of Cell Biology (Anatomy III), Biomedical Center, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany

,

Christian Weber

²DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

³Institute for the Prevention of Cardiovascular Diseases (IPEK), Ludwig-Maximilians-Universität München, Munich, Germany

⁷Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands

,

Steffen Massberg

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

²DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

,

Hellen Ishikawa-Ankerhold^*

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

,

Christian Schulz^*

¹Medizinische Klinik und Poliklinik I, Klinikum der Universität München, Ludwig-Maximilians-Universität München, Munich, Germany

²DZHK (German Center for Cardiovascular Research), Partner Site Munich Heart Alliance, Munich, Germany

› Author Affiliations

Abstract

Coronin-1A (Coro1A) belongs to a family of highly conserved actin-binding proteins that regulate cytoskeletal re-arrangement. In mammalians, Coro1A expression is most abundant in the haematopoietic lineage, where it regulates various cellular processes. The role of Coro1A in platelets has been previously unknown. Here, we identified Coro1A in human and mouse platelets. Genetic absence of Coro1A in mouse platelets inhibited agonist-induced actin polymerization and altered cofilin phosphoregulation, leading to a reduction in spreading and low-dose collagen induced aggregation. Furthermore, Coro1A-deficient mice displayed a defect in ferric chloride-induced arterial thrombosis with prolonged thrombus formation and reduced thrombus size. Immunofluorescence analysis revealed a less compact thrombus structure with reduced density of platelets and fibrinogen. In summary, Coro1A has a role in platelet biology with impact on spreading, aggregation and thrombosis.

Keywords

coronin-1A - coro1A - platelets - thrombosis - actin

Full Text

References

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Supplementary Material

Supplementary Material