Detektion von Arabinogalaktan-Proteinen (AGPs) in der Heilpflanze Echinacea purpurea mittels Immunfluoreszenz

Zusammenfassung

Fertigarzneimittel aus dem Roten Sonnenhut werden als unspezifische Immunstimulanzien verwendet, wobei Hinweise bestehen, dass hochmolekulare AGPs an der Wirkung beteiligt sind. Strukturell gehören die AGPs aus Kraut und Wurzel von Echinacea purpurea zu den klassischen AGPs mit Typ-II-Arabinogalaktan-Einheiten. Eine neu entwickelte Methode der Immunfluoreszenz ermöglicht es, AGPs im pflanzlichen Gewebe zu detektieren. Sowohl in der Wurzel als auch in oberirdischen Teilen von Echinacea purpurea finden sich diese Glykoproteine v.a. in den Leitbündeln der Zentralzylinder und dort hauptsächlich im Bereich der Wasserleitbahnen (Xylem). Die höchsten Konzentrationen an AGPs zeigen sich in den Zellwänden der Tracheiden und Tracheen sowie im Bereich der Tüpfelkanäle, welche benachbarte Wasserleitbahnen miteinander verbinden. In der Pflanze scheinen AGPs an der Entwicklung der wasserleitenden Zellen beteiligt zu sein.

Summary

Arabinogalactan-proteins (AGPs) in the medicinal plant Echinacea purpurea

Echinacea products are used as unspecific immunostimulants and contain high molecular weight arabinogalactan-proteins, which possibly are involved in the immunomodulating effects. These AGPs from root and aerial parts of Echinacea purpurea are classical arabinogalactan-proteins with type II arabinogalactans. For microscopic localization of different arabinogalactan-proteins in plant tissue, we developed a new method using immunofluorescence. Arabinogalactan-proteins were found in higher concentrations in the vascular bundles of the central cylinder of roots and aerial parts, especially in the area of the xylem. The most intense labelling was found in the cell walls of fully differentiated vessels and tracheids as well as in the cell wall area of the pit canals connecting adjacent vessels. In the plant, arabinogalactan proteins play a role in the development of the xylematic tissue.

Schlüsselwörter

Echinacea purpurea - Mikroskopie - Immunmarkierung - Arabinogalaktan-Proteine

Keywords

Echinacea purpurea - microscopy - immunolocalization - arabinogalactan-proteins

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