CC BY-NC-ND 4.0 · Laryngorhinootologie 2021; 100(S 02): S124
DOI: 10.1055/s-0041-1727968
Abstracts
Head-Neck-Oncology: HPV / Tumor Marker

Tumor-exclusive peptides from shared and individual antigens in the HLA-ligandome of oropharyngeal squamous cell carcinoma

S Laban
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
J Ezic
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
L Bichmann
2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen
,
D Engelhardt
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
HA. Kestler
4   Universität Ulm, Institut für Medizinische Systembiologie, Ulm
,
A von Witzleben
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
J Thomas
5   University of Southampton, Faculty of Medicine, Cancer Sciences Unit, Southampton, United Kingdom
,
J Mytilineos
6   Deutsches Rotes Kreuz Blutspendedienst, Institut für Klinische Transfusionsmedizin und Immungenetik, Ulm
,
D Fürst
6   Deutsches Rotes Kreuz Blutspendedienst, Institut für Klinische Transfusionsmedizin und Immungenetik, Ulm
,
J Döscher
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
PJ. Schuler
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
C Brunner
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
TK. Hoffmann
1   Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Ulm
,
H-G Rammensee
2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen
,
S Stevanovic
2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen
,
L Mühlenbruch
2   Universität Tübingen, Interfakultäres Institut für Zellbiologie, Abteilung Immunologie, Tübingen
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    Introduction Cancer-antigens are presented to the immune system via Human leucocyte antigens (HLA). The HLA-ligandome in oropharyngeal squamous cell carcinoma (OPSCC) has not been described previously.

    Material and Methods OPSCC tumor biopsies from 40 patients and tonsillar tissue from 5 healthy donors were analyzed. HLA molecules were extracted from fresh frozen OPSCC biopsies, HLA ligands were isolated by high performance liquid chromatography (HPLC) and analyzed by tandem mass spectrometry (MS/MS). Tumor-exclusive peptides (TEP) were identified by comparative profiling against normal tonsil tissue and an in-house benign and malignant HLA-ligandome database. Whole exome sequencing of RNA and DNA was also performed.

    Results OPSCC were classified as HPV+ (n=22) or HPV- (n=18) based on RNA-seq data. In total, 25228 HLA class I presented peptides from 9485 source proteins and 15203 HLA class II presented peptides from 4634 source proteins were detected. Peptides derived from the cancer-testis antigens IMP3, KDM5B, CTNNA2 and ATAD2 were found in = 30 %  of patients. In total, 3746 HLA class I TEP and 5096 HLA class II TEP were identified. Some TEP were only found in HPV+ or HPV-, whereas others were shared among both, HPV+ and HPV-.

    Conclusion The HLA-ligandome of OPSCC contains both shared and individual TEP and differs by HPV-status. Based on these results, semi-personalized or personalized cancer vaccines could be developed.

    Poster-PDF A-1495.pdf

    DFG (GRK-2254), Universität Ulm (Clinician Scientist Programme)


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    Conflict of interest

    The first author points out the following conflict of interest: Simon Laban: Advisory Boards: Merck Sharp & Dohme (MSD), Bristol Myers Squibb (BMS), Astra Zeneca (AZ). Honoraria: MSD, BMS, AZ, Merck Serono.Johannes Döscher: Advisory Boards: Merck Serono. MSD. Honoraria: Merck Serono.Patrick J. Schuler: Advisory Boards: BMS, MSD.Thomas K. Hoffmann: Advisory Boards: MSD, BMS. Honoraria: MSD, BMS, Merck Serono.

    Address for correspondence

    Privatdozent Dr. med. Laban Simon
    Universitätsklinik Ulm, Klinik für Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie
    Ulm

    Publikationsverlauf

    Artikel online veröffentlicht:
    13. Mai 2021

    © 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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