Osteologie 2021; 30(04): 340-341
DOI: 10.1055/s-0041-1736728
Abstract

Cell-cell communication between chondrocytes and osteoblasts through miR-221-3p loaded extracellular vesicles

X Shang
1   Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Robert Koch Straße 40, 37075 Göttingen, Germany
,
O K Böker
1   Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Robert Koch Straße 40, 37075 Göttingen, Germany
,
S Taheri
1   Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Robert Koch Straße 40, 37075 Göttingen, Germany
,
W Lehmann
1   Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Robert Koch Straße 40, 37075 Göttingen, Germany
,
F A Schilling
1   Department of Trauma Surgery, Orthopedics and Plastic Surgery, University Medical Center Göttingen, Robert Koch Straße 40, 37075 Göttingen, Germany
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Introduction

Osteoarthritis (OA) is considered as a whole joint disease. The close physical association between subchondral bone and cartilage suggests the existence of biochemical and molecular crosstalk across the bone-cartilage interface, which may even be elevated in OA [1] [2]. miR-221-3p was demonstrated to be mechanosensitive in cartilage chondrocytes and extracellular vesicles (EVs) have been deeply researched for a specific role in cell-cell communication [3] [4] [5]. The present study is to investigate the communication between chondrocytes and osteoblasts through miR-221-3p transferred via EVs.


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Material and Methods

Chondrocytes and osteoblasts were isolated from newborn rats. EVs were isolated by differential ultracentrifugation. Nanoparticle tracking analysis, western blot analysis and transmission electron microscopy were used to identify EVs. Chondrocytes and osteoblasts were cocultured in transwell and qRT-PCR was used to assess their communication. Osteoblasts were treated with EVs isolated from chondrocytes and results were evaluated by histochemistry methods and qRT-PCR.


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Results

Collagen II immunofluorescence was positive in chondrocytes and mineralized nodule formation of osteoblasts was illustrated by Von Kossa and Alizarin red staining. miR-221-3p was transferred by EVs from chondrocytes to osteoblasts in our coculture transwell approach. Furthermore, qRT-PCR and Alizarin red staining demonstrated a decreased osteogenic capacity of osteoblasts treated with miR-221-3p loaded EVs.


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Conclusion

This study provides a novel perspective on the mechanotransductive effect of miR-221-3p between chondrocytes and osteoblasts' communication through EVs. These results build the basis to modulate bone-cartilage remodeling and communication in the future.


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Conflict of Interest

No potential conflict of interest was reported by the authors.

  • References

  • 1 Taheri et al. Calcif Tissue Int 2021; 1: 1–15
  • 2 Yuan et al. Osteoarthritis Cartilage 2014; 22: 1077–1089
  • 3 Zheng et al. J Mol Med Berl Ger 2017; 95: 615–627
  • 4 Hecht et al. Osteoarthritis Cartilage 2019; 27: 1208–1218
  • 5 Mohd Noor et al. Cells 2021; 10: 1–22

Publication History

Article published online:
04 November 2021

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  • References

  • 1 Taheri et al. Calcif Tissue Int 2021; 1: 1–15
  • 2 Yuan et al. Osteoarthritis Cartilage 2014; 22: 1077–1089
  • 3 Zheng et al. J Mol Med Berl Ger 2017; 95: 615–627
  • 4 Hecht et al. Osteoarthritis Cartilage 2019; 27: 1208–1218
  • 5 Mohd Noor et al. Cells 2021; 10: 1–22