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Exp Clin Endocrinol Diabetes 2017; 125(05): 335-341
DOI: 10.1055/s-0042-105279
Article
© Georg Thieme Verlag KG Stuttgart · New York

Impact of the Ile105Val Polymorphism of the Glutathione S-transferase P1 (GSTP1) Gene on Obesity and Markers of Cardiometabolic Risk in Young Adult Population

E. O. Chielle
1   Center for Health Sciences, Federal University of Santa Maria, UFSM, Santa Maria, RS, Brazil
3   Laboratory of Molecular Biology, University of Western Santa Catarina, UNOESC, São Miguel do Oeste – SC – Brazil
,
A. Trott
3   Laboratory of Molecular Biology, University of Western Santa Catarina, UNOESC, São Miguel do Oeste – SC – Brazil
,
B. da Silva Rosa
3   Laboratory of Molecular Biology, University of Western Santa Catarina, UNOESC, São Miguel do Oeste – SC – Brazil
,
J. N. Casarin
3   Laboratory of Molecular Biology, University of Western Santa Catarina, UNOESC, São Miguel do Oeste – SC – Brazil
,
P. C. Fortuna
3   Laboratory of Molecular Biology, University of Western Santa Catarina, UNOESC, São Miguel do Oeste – SC – Brazil
,
I. B. M. da Cruz
2   Center of Natural and Exact Sciences, Federal University of Santa Maria, UFSM, Santa Maria, RS, Brazil
,
M. B. Moretto
1   Center for Health Sciences, Federal University of Santa Maria, UFSM, Santa Maria, RS, Brazil
,
R. N. Moresco
1   Center for Health Sciences, Federal University of Santa Maria, UFSM, Santa Maria, RS, Brazil
› Author Affiliations
Further Information

Publication History

received 26 September 2015
first decision 26 February 2016

accepted 17 March 2016

Publication Date:
22 September 2016 (online)

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Abstract

The aim of the study was to investigate the association between Glutathione S-transferase P1 (GSTP1) gene polymorphism with obesity and markers of cardiometabolic risk. A cross-sectional study was carried out in individuals aged≥18 and ≤30 years. The study included 54 normal weight, 27 overweight and 68 obese volunteers. Anthropometric measurements and biochemical parameters were evaluated, the DNA was extracted from blood samples and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to measure GSTP1 Ile105Val gene polymorphism of the study participants. Also, biochemical analysis and hormone assays were carried out. A positive association between GSTP1 polymorphism and obesity was observed on subjects carrying at least one G allele (AG and GG). GG genotype was found only in the obese group. The G allele carriers presented 2.4 times higher chance of obesity when compared to those with the AA genotype. These results were independent of sex and age. We suggest that despite a study in population regional (south of Brazil), the GSTP1 gene polymorphism may play a significant role in the increase of susceptibility of obesity and contribute to identify the cardiovascular risk in young adults.

Key words

obesity - glutathione - polymorphism - biomarkers - cardiometabolic
 

  • References

  • 1 Vincent HK, Innes KE, Vincent KR. Oxidative stress and potential interventions to reduce oxidative stress in overweight and obesity. Diabetes Obes Metab 2007; 9: 813-839
    CrossrefPubMedSearch in Google Scholar
  • 2 Higdon JV, Frei B. Obesity and oxidative stress: a direct link to CVD?. Arterioscler ThrombVasc Biol 2003; 23: 365-367
    PubMedSearch in Google Scholar
  • 3 Vincent HK, Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes (Lond) 2006; 30: 400-418
    CrossrefPubMedSearch in Google Scholar
  • 4 Mohn A, Catino M, Capanna R et al. Increased oxidative stress in prepubertal severely obese children: effect of a dietary restriction-weight loss program. J Clin Endocrinol Metab 2005; 90: 2653-2658
    CrossrefPubMedSearch in Google Scholar
  • 5 Cotton SC, Sharp L, Little J et al. Glutathione S transferase polymorphisms and colorectal cancer. Am J Epidemiol 2000; 151: 7-32
    CrossrefPubMedSearch in Google Scholar
  • 6 Wang G, Zhang L, Li Q. Genetic polymorphisms of GSTT1, GSTM1, and NQO1 genes and diabetes mellitus risk in Chinese population. Biochem Biophys Res Commun 2006; 341: 310-313
    CrossrefPubMedSearch in Google Scholar
  • 7 Mahmoud AA, Maivel HG, Dina MA et al. Evaluation of glutathione S-transferase P1 genetic variants affecting type-2 diabetes susceptibility and glycemic control. Arch Med Sci 2012; 4: 631-636
    CrossrefPubMedSearch in Google Scholar
  • 8 Yanlong Y, Xian L. The association between the GSTP1 A313G and GSTM1 null/present polymorphisms and the treatment response of the platinum-based chemotherapy in non-small cell lung cancer (NSCLC) patients: a meta-analysis. 2014; 35: 6791-6799
    PubMedSearch in Google Scholar
  • 9 Tang ZH, Zhang C, Cheng P et al. Glutathione-S-Transferase Polymorphisms (GSTM1, GSTT1 and GSTP1) and Acute Leukemia Risk in Asians: a Meta-analysis. Asian Pac J Cancer Prev 2014; 15: 2075-2081
    CrossrefPubMedSearch in Google Scholar
  • 10 Nomani H, Mozafari H, Ghobadloo SM et al. The association between GSTT1, M1, and P1 polymorphisms with coronary artery disease in Western Iran. Mol Cell Biochem 2011; 354: 181-187
    CrossrefPubMedSearch in Google Scholar
  • 11 De Lima MFA, Bertuzzo CS, Secolin R et al. Genetic interaction of GSH metabolic pathway genes in cystic fibrosis. BMC Medical Genetics 2013; 14: 60
    PubMedSearch in Google Scholar
  • 12 World Health Organization. Obesity: preventing and managing the global epidemic. Report of a World Health Organization Consultation. World Health Organ Tech Rep Ser 2000; 894 i–xii 1-253
    PubMedSearch in Google Scholar
  • 13 Weiner JS, Lourie JA. Practical Human Biology. London: Academic Press; 1981: 439
    Search in Google Scholar
  • 14 Medrano JF, Aasen E, Sharrow L. DNA extraction from nucleated red blood cells. Biotechniques 1990; 8: 43
    PubMedSearch in Google Scholar
  • 15 Miller AS, Dykes DD, Polesky HF. A simple salting-out procedure for extracting DNA from nucleated cells. Nucleic Acids Res 1988; 16: 1215
    CrossrefPubMedSearch in Google Scholar
  • 16 Katz A, Nambi SS, Mather K et al. Quantitative insulin sensitivity check inex: a simple, accurate method for assessing insulin sensitivity in humans. The Journal of Clinical Endocrinology and Metabolism 2000; 85: 2402-2410
    CrossrefPubMedSearch in Google Scholar
  • 17 Lapenna GD, Ciofani SD, Pierdomenico MA et al. Reaction conditions affecting the relationship between thiobarbituric acid reactivity and lipid peroxides in human plasma. Free Radic Biol Med 2001; 31: 331-335
    CrossrefPubMedSearch in Google Scholar
  • 18 Boyne AF, Ellman GL. A methodology for analysis of tissue sulfhydryl components. Analytical Biochemistry 1972; 6: 639-653
    PubMedSearch in Google Scholar
  • 19 Gaspar P, Moreira J, Kvitko K et al. CYP1A1, CYP2E1, GSTM1, GSTT1, GSTP1, and TP53 Polymorphisms: They Indicate Susceptibility to Chronic Obstructive Pulmonary Disease and Non-Small-Cell Lung Cancer?. Genetics and Molecular Biology 2004; 27: 133-138
    CrossrefPubMedSearch in Google Scholar
  • 20 Mo Z, Gao Y, Cao Y et al. An Up dating Meta-Analysis of the GSTM1,GSTT1, and GSTP1 Polymorphisms and Prostate Cancer: a HuGE review. The Prostate 2009; 69: 662-688
    CrossrefPubMedSearch in Google Scholar
  • 21 Piva SJ, Duarte MM, Da Cruz IB et al. Ischemia-modified albumin as an oxidative stress biomarker in obesity. Clin Biochem 2011; 44: 345-347
    CrossrefPubMedSearch in Google Scholar
  • 22 Gottlieb MG, Schwanke CH, Santos AF et al. Association among oxidized LDL levels, MnSOD, apolipoproteinE polymorphisms, and cardiovascular risk factors in a south Brazilian region population. Genet Mol Res 2005; 4: 691-703
    PubMedSearch in Google Scholar
  • 23 Montano MA, Da Cruz IB, Duarte MM et al. Inflammatory cytokines in vitro production are associated with Ala16Val superoxide dismutase gene polymorphism of peripheral blood mononuclear cells. Cytokine 2012; 60: 30-33
    CrossrefPubMedSearch in Google Scholar
  • 24 Kim JH, Park SG, Lee KH et al. GSTM1 and GSTP1 polymorphisms as Potential Factors for Modifying the Effect of Smoking on Inflammatory response. J Korean Med Sci 2006; 21: 1021-1027
    CrossrefPubMedSearch in Google Scholar
  • 25 Ramprasath T, SenthilMurugan P, Prabakaran AD et al. Potential risk modifications of GSTT1, GSTM1 and GSTP1 (glutathione-S-transferases) variants and their association to CAD in patients with type-2 diabetes. Biochem Biophys Res Commun 2011; 407: 49-53
    CrossrefPubMedSearch in Google Scholar
  • 26 Moussa SA. Oxidative stress in Diabetes mellitus. Romanian J Biophys 2008; 18: 225-236
    PubMedSearch in Google Scholar
  • 27 Hayes JD, Pulford DJ. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol 1995; 30: 445-600
    CrossrefPubMedSearch in Google Scholar
  • 28 Yalin S, Hatungil R, Tamer L et al. Glutathione S-transferase gene polymorphisms in Turkish patients with diabetes mellitus. Cell Biochem Funct 2007; 25: 509-513
    CrossrefPubMedSearch in Google Scholar
  • 29 Al-Rawi NH. Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. DiabVasc Dis Res 2011; 8: 22-28
    PubMedSearch in Google Scholar
  • 30 Sorrentino SA, Besler C, Rohrer L et al. Endothelial vasoprotective effects of high-density lipoprotein are impaired in patients with type 2 diabetes mellitus but are improved after extended-release niacin therapy. Circulation 2010; 121: 110-122
    CrossrefPubMedSearch in Google Scholar
  • 31 Rotter V, Nagaev I, Smith U. Interleukin-6 (IL-6) induce insulin resitence in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overespressed in human fat cells from insulin-resistant subjects. J Biol Chem 2003; 278: 45777-45784
    CrossrefPubMedSearch in Google Scholar
  • 32 Kahn BB, Flier JS. Obesity and insulin resistance. J Clin Investig 2000; 106: 473-481
    CrossrefPubMedSearch in Google Scholar
  • 33 Giron MD, Salto R, Gonzalez Y. Modulation of hepatic and intestinal glutathione S-transferases and other antioxidant enzymes by dietary lipids in streptozotocin diabetic rats. Chemosphere 1999; 38: 3003-3013
    CrossrefPubMedSearch in Google Scholar
  • 34 Karalis KP, Giannogonas P, Kodela E et al. Mechanisms of obesity and related pathology: linking immune responses to metabolic stress. FEBS 2009; 276: 5747-5747
    CrossrefPubMedSearch in Google Scholar