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Planta Med 2017; 83(01/02): 57-62
DOI: 10.1055/s-0042-107673
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antileishmanial Activity of a Calophyllum brasiliense Leaf Extract

Bruna Muller Cardoso
1   Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil
,
Tatiane Franca Perles De Mello
1   Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil
,
Daniele Stefani Lopes Lera
1   Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil
,
Mislaine Adriana Brenzan
2   Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, PR, Brazil
,
Diógenes Aparecido Garcia Cortez
2   Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá, Maringá, PR, Brazil
,
Lucélia Donatti
3   Programa de Pós-Graduação em Biologia Celular e Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil
,
Thais Gomes Verzignassi Silveira
1   Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil
,
Maria Valdrinez Campana Lonardoni
1   Programa de Pós-Graduação em Biociências e Fisiopatologia, Departamento de Análises Clínicas e Biomedicina, Universidade Estadual de Maringá, Maringá, PR, Brazil
› Author Affiliations
Further Information

Publication History

received 04 August 2015
revised 10 April 2016

accepted 15 April 2016

Publication Date:
25 May 2016 (online)

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Abstract

Calophyllum brasiliense (Clusiaceae) is a tree that grows mainly in the Atlantic Forest in Brazil. Its leaves possess antibacterial activity, cytotoxic activity against certain tumor cell lines, and antimicrobial activity in BALB/c mice infected with Leishmania (Leishmania) amazonensis.

Aiming to identify ultrastructural changes and DNA fragmentation in Leishmania (Viannia) braziliensis, promastigotes were treated with a concentration of the dichloromethane extract and coumarin (−) mammea A/BB from C. brasiliense leaves that inhibited 50 % of the parasites (IC50), and were evaluated by transmission and scanning electron microscopy. Ultrastructural changes showed different levels of mitochondrial alterations, including mitochondrial swelling and a reduction in the density of the mitochondrial matrix. Induced DNA fragmentation, as observed by TUNEL, suggested that the extract and coumarin (−) mammea A/BB induced apoptosis-like cell death. These results suggest that the combination of C. brasiliense extract and coumarin (−) mammea A/BB can be considered a promising candidate for the development of new antiprotozoal agents, because of its significant leishmanicidal activity.

Key words

Calophyllum brasiliense - Clusiaceae - cutaneous leishmaniasis - Leishmania (Viannia) braziliensis - ultrastructure - DNA fragmentation - coumarin (−) mammea A/BB
 

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