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Drug Res (Stuttg) 2016; 66(08): 432-435
DOI: 10.1055/s-0042-108640
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

The Effect of Prednisolone on miR 15a and miR16-1 Expression Levels and Apoptosis in Acute Lymphoblastic Leukemia Cell Line: CCRF-CEM

A. Azimi
1   Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
,
M. F. Hagh
2   Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
,
B Yousefi
3   Department of Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
,
M. A. Rahnama
4   Department of Hematology, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
5   School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
A. Khorrami
1   Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
,
M. Z. Heydarabad
2   Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
,
M. Najafpour
6   Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran
,
J. Hallajzadeh
1   Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
,
A. Ghahremani
1   Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
› Author Affiliations
Further Information

Publication History

received 16 April 2016

accepted 10 May 2016

Publication Date:
09 June 2016 (online)

Also available at
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Abstract

Background: Glucocorticoids are probably the most important drugs in the treatment of childhood acute lymphoblastic leukemia (ALL). Prednisolone exerts its effect by induce apoptosis in lymphoid lineage cells. Micro RNAs are 18–24 nucleotides RNA implicated in the control of essential biological functions, including apoptosis. In the following study, the effect of prednisolone on the expression of miR 15a & miR16-1 and apoptosis in the CCRF-CEM cell line is investigated.

Methods: The cell line of CCRF-CEM was cultured in standard conditions. The changes in the miR 15a and miR 16-1 expression levels were determined by Real Time-PCR technique. Also, the apoptosis is evaluated by flow cytometry using Annexin V and PI staining.

Results: This study revealed that, the prednisolone induced apoptosis in a time dependent manner. Prednisolone in concentration of 700 µM was significantly increased the expression of miR 16-1 and miR 15a after 24 h and 48 h treatment (p<0.05).

Conclusion: prednisolone-induced apoptosis might be mediated by up-regulation of these 2 miRNAs in CCRF-CEM cells.

Key words

ALL - prednisolone - miR 15a - miR 16-1 - apoptosis
 

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