Genotype-dependent Metabolic Responses to Semi-Purified High-Sucrose High-Fat Diets in the TALLYHO/Jng vs. C57BL/6 Mouse during the Development of Obesity and Type 2 Diabetes

J. K. Parkman; X. Mao; K. Dillon; A. Gudivada; N. Moustaid-Moussa; A. M. Saxton; J. H. Kim

doi:10.1055/s-0042-109605

Experimental and Clinical Endocrinology & Diabetes, Table of Contents

Exp Clin Endocrinol Diabetes 2016; 124(10): 622-629
DOI: 10.1055/s-0042-109605

Article

Genotype-dependent Metabolic Responses to Semi-Purified High-Sucrose High-Fat Diets in the TALLYHO/Jng vs. C57BL/6 Mouse during the Development of Obesity and Type 2 Diabetes

J. K. Parkman

¹Pharmacology, Physiology and Toxicology, Marshall University, Huntington, United States

,

X. Mao

¹Pharmacology, Physiology and Toxicology, Marshall University, Huntington, United States

,

K. Dillon

¹Pharmacology, Physiology and Toxicology, Marshall University, Huntington, United States

,

A. Gudivada

¹Pharmacology, Physiology and Toxicology, Marshall University, Huntington, United States

,

N. Moustaid-Moussa

²Department of Nutritional Sciences, Texas Tech University, Lubbock, United States

,

A. M. Saxton

³Department of Animal Science, University of Tennessee, Knoxville, United States

,

J. H. Kim

¹Pharmacology, Physiology and Toxicology, Marshall University, Huntington, United States

› Author Affiliations

Abstract

Background: The co-epidemic of obesity and type 2 diabetes is associated with increased morbidity and mortality. Genetic factors are highly involved in the development of these diseases, in the form of interactions of multiple genes within obesogenic and diabetogenic environments, such as a high fat diet. The TALLYHO/Jng (TH) mouse is an inbred polygenic model for human obesity and type 2 diabetes. In order to further develop the TH mouse as a clinically relevant model, we investigated diet dependence of obesity and type 2 diabetes in TH mice vs. C57BL/6 (B6) mice.

Results: TH and B6 mice were weaned onto a standard rodent chow, semi-purified high-sucrose low-fat (HSLF), or semi-purified high-sucrose high-fat (HSHF) diet and maintained on these diets throughout the study. Despite similar fat contents in HSLF diets and chow, both B6 and TH mice responded to HSLF diets, with increases in adiposity. TH mice, but not B6 mice, exhibited significantly higher adiposity with severely aggravated glucose intolerance and hyperglycemia on HSHF diets compared to the other diets. HSLF diets also advanced diabetes in TH mice compared to chow, but it did not surpass the effects of HSHF diets. The severe glucose intolerance and hyperglycemia in TH mice on both HSLF and HSHF diets were accompanied by significantly reduced Glut4 mRNA levels compared to B6 mice.

Conclusions: The present data demonstrate that diets are important modulators of genetic susceptibility to type 2 diabetes and obesity in TH mice. The interplay between heredity and dietary environment in TH mice appears to amplify insulin resistance, contributing to severe glucose intolerance and diabetes.

Key words

high fat diet - high sucrose diet - obesity - type 2 diabetes - genetics - TALLYHO

Full Text

References

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