Aerobic Exercise Decreases Lung Inflammation by IgE Decrement in an OVA Mice Model

Deborah Camargo Hizume-Kunzler; Flavia R. Greiffo; Bárbara Fortkamp; Gabriel Ribeiro Freitas; Juliana Keller Nascimento; Thayse Regina Bruggemann; Leonardo Melo Avila; Adenir Perini; Franciane Bobinski; Morgana Duarte Silva; Fernanda Rocha Lapa; Rodolfo Paula Vieira; Verônica Vargas Horewicz; Adair Roberto Soares dos Santos; Kelly Cattelan Bonorino

doi:10.1055/s-0042-121638

International Journal of Sports Medicine, Inhaltsverzeichnis

Int J Sports Med 2017; 38(06): 473-480
DOI: 10.1055/s-0042-121638

Immunology

Aerobic Exercise Decreases Lung Inflammation by IgE Decrement in an OVA Mice Model

Deborah Camargo Hizume-Kunzler

¹State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil

,

Flavia R. Greiffo

²Nove de Julho University – UNINOVE, Research, São Paulo, Brazil

,

Bárbara Fortkamp

¹State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil

,

Gabriel Ribeiro Freitas

³Universidade do Estado de Santa Catarina Centro de Ciencias da Departamento de Fisioterapia, Saude e do Esporte, Florianopolis, Brazil

,

Juliana Keller Nascimento

³Universidade do Estado de Santa Catarina Centro de Ciencias da Departamento de Fisioterapia, Saude e do Esporte, Florianopolis, Brazil

,

Thayse Regina Bruggemann

⁴School of Medicine of University of Sao Paulo, Internal Medicine, Sao Paulo, Brazil

,

Leonardo Melo Avila

¹State University of Santa Catarina, Physical Therapy, Florianopolis, Brazil

,

Adenir Perini

⁵Faculdade de Medicina da Universidade de São Paulo, Departamento de Clínica Médica, São Paulo, Brazil

,

Franciane Bobinski

⁶Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil

,

Morgana Duarte Silva

⁶Department of Biological Science, Federal University of Santa Catarina, Florianopolis, Brazil

,

Fernanda Rocha Lapa

⁷Universidade do Contestado Campus Concordia, Ciências Biológicas, Concordia, Brazil

,

Rodolfo Paula Vieira

⁸Nove de Julho University (UNINOVE), Laboratory of Pulmonary and Exercise Immunology (LABPEI) and Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), Sao Paulo, Brazil

,

Verônica Vargas Horewicz

⁹Universidade Federal de Santa Catarina, Departamento de Farmacologia, Florianopolis, Brazil

,

Adair Roberto Soares dos Santos

¹⁰Universidade Federal de Santa Catarina, Departamento de Ciências Biológicas, Florianópolis, Brazil

,

Kelly Cattelan Bonorino

¹⁰Universidade Federal de Santa Catarina, Departamento de Ciências Biológicas, Florianópolis, Brazil

› Institutsangaben

Abstract

Aerobic exercise (AE) reduces lung function decline and risk of exacerbations in asthmatic patients. However, the inflammatory lung response involved in exercise during the sensitization remains unclear. Therefore, we evaluated the effects of exercise for 2 weeks in an experimental model of sensitization and single ovalbumin-challenge. Mice were divided into 4 groups: mice non-sensitized and not submitted to exercise (Sedentary, n=10); mice non-sensitized and submitted to exercise (Exercise, n=10); mice sensitized and exposed to ovalbumin (OVA, n=10); and mice sensitized, submitted to exercise and exposed to OVA (OVA+Exercise, n=10). 24 h after the OVA/saline exposure, we counted inflammatory cells from bronchoalveolar fluid (BALF), lung levels of total IgE, IL-4, IL-5, IL-10 and IL-1ra, measurements of OVA-specific IgG1 and IgE, and VEGF and NOS-2 expression via western blotting. AE reduced cell counts from BALF in the OVA group (p<0.05), total IgE, IL-4 and IL-5 lung levels and OVA-specific IgE and IgG1 titers (p<0.05). There was an increase of NOS-2 expression, IL-10 and IL-1ra lung levels in the OVA groups (p<0.05). Our results showed that AE attenuated the acute lung inflammation, suggesting immunomodulatory properties on the sensitization process in the early phases of antigen presentation in asthma.

Key words

asthma - inflammation - aerobic exercise - IgE

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Referenzen

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