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Exp Clin Endocrinol Diabetes 2017; 125(05): 282-289
DOI: 10.1055/s-0043-100117
Article
© Georg Thieme Verlag KG Stuttgart · New York

Effect of Carnosine on Renal Function, Oxidation and Glycation Products in the Kidneys of High-Fat Diet/Streptozotocin-Induced Diabetic Rats

Abdurrahman Fatih Aydın
1   Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
,
Canan Küçükgergin
1   Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
,
İlknur Bingül
1   Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
,
Işın Doğan-Ekici
2   Department of Pathology, Yeditepe University Medical Faculty, Kayışdağı, Istanbul, Turkey
,
Semra Doğru-Abbasoğlu
1   Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
,
Müjdat Uysal
1   Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey
› Author Affiliations
Further Information

Publication History

received 20 December 2016
revised 20 December 2016

accepted 03 January 2017

Publication Date:
13 April 2017 (online)

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Abstract

Background

High fat diet (HFD) and low dose of streptozotocin (STZ)-treated rats provide an animal model for type 2 Diabetes Mellitus (T2DM). Oxidative stress plays a role in the development of diabetic complications. Carnosine (CAR) has antioxidant and antiglycating properties. We investigated effects of CAR on renal function, oxidation and glycation products in HFD+STZ-rats.

Materials and Methods

Rats were fed with HFD (60% of total calories from fat) for 4 weeks and then a single dose STZ (40 mg/kg; i.p.) was applied. Rats with blood glucose levels above 200 mg/dL were fed with HFD until the end of the 12th week. CAR (250 mg/kg body weight; i.p.; 5 times a week) was administered to rats for the last 4 weeks. Glycated hemoglobin (HbA1c), glucose, lipids, and andrenal function tests in serum as well as reactive oxygen species, malondialdehyde, protein carbonyl, advanced oxidation protein products, advanced glycation end products (AGEs), antioxidant power, and antioxidant enzyme activities and their mRNA expressions in kidneys were determined.

Results

CAR treatment did not alter glucose and HbA1c, but it decreased serum lipids, creatinine, and urea levels in HFD+STZ rats. Oxidation products of lipids and proteins and AGEs levels decreased, but antioxidant enzyme activities and their mRNA expressions remained unchanged due to CAR treatment.

Conclusion

Our results indicate that CAR treatment alleviated renal function and decreased accumulation of oxidation and glycation products in kidneys in HFD+STZ-rats.

Key words

carnosine - high fat diet - streptozotocin - oxidative stress - advanced glycation end products - renal function
 

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