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Horm Metab Res 2017; 49(06): 457-465
DOI: 10.1055/s-0043-108250
Endocrine Research
© Georg Thieme Verlag KG Stuttgart · New York

Dietary Isoflavone-Dependent and Estradiol Replacement Effects on Body Weight in the Ovariectomized (OVX) Rat

Ashley L. Russell   *
1   Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Jamie Moran Grimes   *
1   Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
2   Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Danette F. Cruthirds
1   Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Joanna Westerfield
2   Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Lawren Wooten
2   Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Margaret Keil
3   NIH – NICHD, Bethesda, MD, USA
,
Michael J. Weiser
4   DSM Nutritional Products Inc., Human Nutrition & Health, Boulder, CO, USA
,
Michael R. Landauer
5   Armed Forces Radiobiology Research Institute, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Robert J. Handa
6   Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA
,
T. John Wu
1   Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
2   Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
,
Darwin O. Larco
2   Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA
› Author Affiliations
Further Information

Publication History

received 07 September 2016

accepted 28 March 2017

Publication Date:
08 May 2017 (online)

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Abstract

17β-Estradiol is known to regulate energy metabolism and body weight. Ovariectomy results in body weight gain while estradiol administration results in a reversal of weight gain. Isoflavones, found in rodent chow, can mimic estrogenic effects making it crucial to understand the role of these compounds on metabolic regulation. The goal of this study is to examine the effect of dietary isoflavones on body weight regulation in the ovariectomized rat. This study will examine how dietary isoflavones can interact with estradiol treatment to affect body weight. Consistent with previous findings, animals fed an isoflavone-rich diet had decreased body weight (p<0.05), abdominal fat (p<0.05), and serum leptin levels (p<0.05) compared to animals fed an isoflavone-free diet. Estradiol replacement resulted in decreased body weight (p<0.05), abdominal fat (p<0.05), and serum leptin (p<0.05). Current literature suggests the involvement of cytokines in the inflammatory response of body weight gain. We screened a host of cytokines and chemokines that may be altered by dietary isoflavones or estradiol replacement. Serum cytokine analysis revealed significant (p<0.05) diet-dependent increases in inflammatory cytokines (keratinocyte-derived chemokine). The isoflavone-free diet in OVX rats resulted in the regulation of the following cytokines and chemokines: interleukin-10, interleukin-18, serum regulated on activation, normal T cell expressed and secreted, and monocyte chemoattractant protein-1 (p<0.05). Overall, these results reveal that estradiol treatment can have differential effects on energy metabolism and body weight regulation depending on the presence of isoflavones in rodent chow.

Key words

energy metabolism - leptin - cytokines

* These authors contributed equally to this work


 

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