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Neuropediatrics 2024; 55(05): 327-336
DOI: 10.1055/s-0044-1788032
Original Article

Dysregulated Apoptosis and Autophagy in Childhood Epilepsy: Correlation to Clinical and Pharmacological Patterns

Ahmed El-Abd Ahmed
1   Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt
,
Mohammed H. Hassan 
2   Department of Medical Biochemistry, Faculty of Medicine, South Valley University, Qena, Egypt
,
Asmaa A. Abdelfatah
1   Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt
,
Ali Helmi Bakri
1   Department of Pediatrics, Faculty of Medicine, South Valley University, Qena, Egypt
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Abstract

Objectives We aimed to assess the serum levels of caspase-3 as a marker of apoptosis and microtubule-associated protein 1A/1B-light chain 3 (MAP1-LC3) as an autophagy marker in epileptic children with various clinical and pharmacological types.

Methods This case–control study was carried out on 90 participants (50 pediatric patients with epilepsy and 40 healthy matched children), the patients were categorized into three groups: Group (A): 25 pharmacosensitive epilepsy, Group (B): 25 pharmacoresistant epilepsy, and Group (C): 40 (age, sex, and body mass index) matched healthy children selected as controls. Serum caspase-3 and MAP1-LC3 were measured in all study groups, using commercially available ELISA kits.

Results Serum caspase-3 was significantly higher among epileptic children, especially in the pharmacoresistant group, cases managed with multiple antiepileptic drugs, and cases with abnormal EEG findings. Conversely, circulating MAP1-LC3 levels showed a significant reduction in epilepsy cases, particularly in pharmacoresistant cases, in cases treated with multiple antiepileptic drugs, and in cases with abnormal EEG data. A significant negative correlation between serum caspase-3 and MAP1-LC3 was found among epileptic children (r =  −0.369, p = 0.0083). Serum caspase-3 was a more valid biomarker in helping diagnose childhood epilepsy, while serum MAP1-LC3 was more valid in predicting pharmacoresistant type.

Conclusion The study reveals that serum caspase-3 levels were significantly elevated, particularly in pharmacoresistant cases and those managed with multiple drugs. Conversely, MAP1-LC3 levels were significantly reduced in epilepsy cases, suggesting potential involvement of altered apoptosis and autophagy in childhood epilepsy.

Keywords

childhood epilepsy - apoptosis - autophagy - caspase-3 - MAP1-LC3

Ethical Approval and Consent to Participate

Cases and controls were collected correspondingly with the guidelines established in the Declaration of Helsinki. This study was approved by the Ethics Committee of the Faculty of Medicine, South Valley University, Qena, Egypt (Ethical approval code: SVU-MED-PED025-1-23-2-544). Informed written consent was taken from parents or caregivers of the included participants for participation in the study and publication.


Availability of Data and Materials

The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request, after obtaining the permission of our institute.


Authors' Contributions

Study concept and design: A.E-A.A., M.H.H., A.H.B. Literature research, statistical analysis, and data interpretation: A.E-A.A., M.H.H., A.A.A., A.H.B. Selection of the participants and their clinical evaluation: A.E-A.A., A.A.A., A.H.B. Biochemical assays: M.H.H. First draft of the manuscript: M.H.H. All authors approved the final version of the manuscript.




Publication History

Received: 28 March 2024

Accepted: 06 June 2024

Article published online:
04 July 2024

© 2024. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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