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Z Gastroenterol 2000; 38(12): 925-931
DOI: 10.1055/s-2000-10021
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© Karl Demeter Verlag im Georg Thieme Verlag Stuttgart · New York

DNA of nonhuman primates harbors hepatitis C-virus-specific sequences of its 5’-non-coding region (5’-NCR)[*]

R. H. Dennin1 , J. Wo2 , Z. Chen2 , Ch Roos3
  • Institute of Medical Microbiology and Hygiene, Medical University, Luebeck, Germany
  • , Institute of Infectious Diseases, The First Affiliate Hospital, College of Medicine, Zhejiang University, Hangzhou, P. R. China
  • , Deutsches Primatenzentrum, Arbeitsgemeinschaft Genetik, Kellnerweg 4, Göttingen, Germany.
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Publication History

16.3.2000

11.9.2000

Publication Date:
31 December 2000 (online)

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Summary

The DNA from PBMCs of both hepatitis C virus (HCV)-positive patients and healthy HCV-negative human individuals tested thus far contains essential parts - up to 272/341 nucleotides - of the HCV 5’-non-coding region (5’-NCR). These findings bring up the question of the possible evolutionary background of these sequences. Therefore, using the same methodology, we looked for the same sequences in animals closely related to man, i. e., in nonhuman primates (two chimpanzees, one orang-utan, one Debrazza monkey, two New World monkey species and a prosimian). The DNA from PBMCs of the studied animals belonging to nonhuman primates contains essential parts - up to 272/341 nucleotides - of the HCV 5’-non-coding region (5’-NCR). A common sequence of 82 nucleotides is contained in the DNA of all the tested animals but only the chimpanzee‘s DNA harbors the same, longer sequence region of 272/341 nucleotides of the 5’-NCR found in human DNA. The results may provide a clue as to the possible origin of parts of the IRES containing sequence area of the HCV.

Die DNA von nichthumanen Primaten enthält HCV-spezifische Sequencen der 5’-nichtkodierenden Region (5'-NCR)

Die DNA aus PBMCs von Hepatitis-C-Virus-(HCV)positiven Patienten und HCV-negativen Probanden enthält einen Sequenzabschnitt (bis zu 272/341 Nukleotide) der 5’-nichtkodierenden Region (5’-NCR) des Genoms vom Hepatitis-C-Virus. Diese Befunde führten zur Frage des möglichen evolutionären Hintergrundes dieser Sequenzen. Mit derselben Methode, die auch zu Analyse menschlicher DNA angewandt wurde, haben wir die genomische DNA von nichthumanen Primaten untersucht (Schimpansen, Orang-Utan, Debrazza Monkey, 2 Neuweltaffen und ein Prosimian). Ein 82 Nukleotide langer Sequenzbereich ist bei allen untersuchen Tieren vorhanden. Nur in der DNA von Schimpansen ließen sich 272/341 Nukleotide des 5’-NCR nachweisen, die bisher auch bei HCV-negativen Probanden detektiert und analysiert wurden. Diese Befunde könnten auf den möglichen Ursprung von Sequenzbereichen des HCV-Genoms hinweisen, die an der IRES beteiligt sind.

Abbreviations: PBMC, peripheral blood mononuclear cells; IRES, internal ribosome entry site; 5’-NCR, 5’-non-coding region of the hepatitis C virus (HCV) genome; NWM, New World monkeys; OWM, Old World monkeys; PRS, Prosimian; PAC, phage artifical chromosome library; RE, restriction endonuclease; DGKC, Deutsche Gesellschaft für Klinische Chemie.

Key words

Hepatitis C Virus - 5’-non-coding Region - DNA-Sequences - Nonhuman Primates - Phylogenetic Background

Schlüsselwörter

Hepatitis-C-Virus - 5’-nichtkodierende Region - DNA-Sequenzen - nichthumane Primaten - phylogenetischer Hintergrund

1 Parts of this manuscript have been presented at the XIth International Congress of Virology, August 9 to 13, 1999, Sydney, Australia; Abstract No. VP13.04.

1

References

  • 1 Choo Q -L, Kuo G, Weiner A J. et al . Isolation of a cDNA clone derived from a blood-borne Non-A, Non-B viral hepatitis genome.  Science. 1989;  244 359-362
    Google Scholar
  • 2 Choo Q -L, Richman K, Han J H. et al . Genetic organization and diversity of the hepatitis C virus.  Proc Natl Acad Sci USA. 1991;  88 2451-2455
    Google Scholar
  • 3 Bassett S E, Brasky K M, Lanford R E. Analysis of hepatitis C virus-inoculated chimpanzees reveals unexpected clinical profiles.  J Virol. 1998;  72 2589-2599
    Google Scholar
  • 4 Kolykhalov A A, Agapov E V, Blight K J. et al . Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA.  Science. 1997;  277 570-574
    Google Scholar
  • 5 Yanagi M, Purcell R H, Emerson S U, Bukh J. Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee.  Proc Natl Acad Sci USA. 1997;  94 8738-8743
    Google Scholar
  • 6 Beard M, Abell G, Honda M. et al .An infectious molecular clone of a japanese genotype 1b Hepatitis C virus. Abstract VW 13.02 Sydney, Australia; XIth International Congress of Virology August 9-13, 1999
    Google Scholar
  • 7 Hong Z, Beaudet-Miller M, Lanford R E. et al . Generation of transmissible hepatitis C virions from a molecular clone in chimpanzees.  Virology. 1999;  256 36-44
    Google Scholar
  • 8 Dennin R H, Wo J, Chen Z. Hepatitis C virus specific DNA sequences in human DNA: Differentiation by means of restriction enzyme analysis at the DNA level of healthy, anti-HCV negative individuals.  Clin Chem Lab Med. 1999;  37 623-630
    Google Scholar
  • 9 Rolfs A, Schuller I, Finckh U, Weber-Rolfs I. PCR: Clinical Diagnostics and Research. Berlin, Heidelberg; Springer-Verlag 1992 Chapter 9
    Google Scholar
  • 10 Bukh J, Purcell R H, Miller R H. Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay.  Proc Natl Acad Sci USA. 1992;  89 187-191
    Google Scholar
  • 11 Garson J A, Ring C JA, Tuke P W. Improvement of HCV genome detection with ‘short’ PCR products.  Lancet. 1991;  338 1466-1467
    Google Scholar
  • 12 Yousuf  M, Nakano Y, Tanaka E, Sodeyama T, Kiyosawa K. Persistence of viremia in patients with type C chronic hepatitis during long-term follow up.  Scand J Gastroenterol. 1992;  27 812-816
    Google Scholar
  • 13 Sambrook J, Fritsch E F, Maniatis T. Molecular cloning - A laboratory manual. Chapter 5.3. Restriction and DNA methylation enzymes Cold Spring Harbor; Cold Spring Harbor Laboratory Press 1989 2nd ed
    Google Scholar
  • 14 Mishiro S, Hoshi Y, Takeda K. et al . NonA, NonB hepatitis specific antibodies directed at host-derived epitope: Implication for an autoimmune process.  Lancet. 1990;  336 1400-1403
    Google Scholar
  • 15 Dennin R H, Chen Z, Wo J. The GOR47-1 sequence in human DNA encoding for a potential autoantigen in connection with hepatitis C - a sequence not only reserved for humans.  Z Gastroenterol. 1998;  36 803-808
    Google Scholar
  • 16 Uyttendaele S, Claeys H, Mertens W, Verhaert H, Vermylen C. Evaluation of third-generation screening and confirmatory assays for HCV antibodies.  Vox Sang. 1994;  66 122-129
    Google Scholar
  • 17 Vernelen K, Claeys H, Mertens W, Verhaert H, Vermylen C. Significance of NS3 and NS5 antigens in screening for HCV antibody.  Lancet. 1994;  343 853
    Google Scholar
  • 18 LaPerche S, Lemaire J -M, Coste J, Defer C h, Cantaloube J -F. GB virus type C/hepatitis G virus infection in French blood donors with anti-NS5 isolated reactivities by recombinant immunoblot assay for hepatitis C virus.  Transfusion. 1999;  39 790-791
    Google Scholar
  • 19 Lemaire J -M, Courouce A M, Defer C h. et al . HCV RNA in blood donors with isolated reactivities by third generation RIBA.  Transfusion. 2000;  40 867-870
    Google Scholar
  • 20 Major M E, Mihalik K, Fernandez J. et al . Long-term follow up of chimpanzees inoculated with the first infectious clone for hepatitis C virus.  J Virol. 1999;  73 3317-3325
    Google Scholar
  • 21 Smith D B, Mellor J, Jarvis L M. et al . Variation of the hepatitis C virus 5’ non-coding region: Implications for secondary structure, virus detection and typing.  J Gen Virol. 1995;  76 1749-1761
    Google Scholar
  • 22 Stuyver L, Rossau R, Wyseur A. et al . Typing of the hepatitis C virus isolates and characterization of new subtypes using a line probe assay.  J Gen Virol. 1993;  74 1093-1102
    Google Scholar
  • 23 Smith D B, Pathirana S, Davidson F. et al . The origin of hepatitis C virus genotypes.  J Gen Virol. 1997;  78 321-328
    Google Scholar
  • 24 Schneider H, Schneider M P, Sampaio I. et al . Molecular phylogeny of the New Worls monkeys (Platyrrhini, primates).  Mol Phylogenet Evol. 1993;  2 225-242
    Google Scholar
  • 25 Smit A F. The origin of interspersed repeats in the human genome.  Curr Opin Genet Dev. 1996;  6 743-748
    Google Scholar
  • 26 Shapiro J A. The discovery and significance of mobile genetic elements. Sherratt DJ Mobile Genetic Elements Oxford, New York, Tokyo; Oxford University Press 1995: 1-17
    Google Scholar
  • 27 Wilkinson D A, Mager D L, Leong J -AC. Endogenous human retroviruses. Levy J The Retroviridae New York; Plenum Press 1994 Vol 3: 456-535
    Google Scholar
  • 28 Martin J, Hernioou E, Cook J, O’Neill R W, Tristem M. Human endogenous retrovirus type I-related viruses have an apparently widespread distribution within vertebrates.  J Virol. 1997;  71 437-443
    Google Scholar
  • 29 Steinhuber S, Brack M, Hunsmann G. et al . Distribution of human endogenous retrovirus HERV-K genomes in humans and different primates.  Hum Genet. 1995;  96 188-192
    Google Scholar
  • 30 Kim H S, Takenaka O, Crow T J. Isolation and phylogeny of endogenous retrovirus sequences belonging to the HERV-W family in primates.  J Gen Virol. 1999;  80 2613-2619
    Google Scholar
  • 31 Brazas R, Ganem D. A cellular homology of hepatitis delta antigen: Implication for viral replication and evolution.  Science. 1996;  274 90-94
    Google Scholar
  • 32 Miller R H, Purcell R H. Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as of two plant virus supergroups.  Proc Natl Acad Sci USA. 1990;  87 2057-2061
    Google Scholar
  • 33 Ohba K -I, Mizokami M, Lau J YN. et al . Evolutionary relationship of hepatitis C, pesti-, flavi-, plantvirues, and newly discovered GB hepatitis agents.  FEBS Letters. 1996;  378 232-234
    Google Scholar

1 Parts of this manuscript have been presented at the XIth International Congress of Virology, August 9 to 13, 1999, Sydney, Australia; Abstract No. VP13.04.

1

2 Here we only want to present data about these very basic findings of 5’-NCR sequence sections of the expected molecular size in non-human primates. Summarized details of amplified fragments of longer than the expected size from both chimpanzee and orang-utan, in comparision with those found in humans, will be presented elsewhere.

Address for correspondence:

Reinhard H. Dennin

Institute of Medical Microbiology & Hygiene Medizinische Universität zu Lübeck

Ratzeburger Allee 160

23538 Lübeck

Email: dennin@hygiene.mu-luebeck.de

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