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Int J Sports Med 2001; 22(2): 103-110
DOI: 10.1055/s-2001-11365
Physiology and Biochemistry

Georg Thieme Verlag Stuttgart ·New York

Acute Effect of Physical Exercise on Serum Insulin-Like Growth Factor-Binding Protein 2 and 3 in Healthy Men: Role of Exercise-Linked Growth Hormone Secretion

L. Di Luigi1 ,  L. Guidetti1 ,  M. Nordio2 ,  C. Baldari1 ,  F. Romanelli2
  • 1 Laboratory of Endocrine Research, Endocrinology Unit, University Institute of Motor Sciences, Rome, Italy
  • 2 Division of Andrology, University “La Sapienza”, Rome, Italy
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Publication History

Publication Date:
31 December 2001 (online)

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The purpose of this study was to delineate the role of GH on serum IGF-I, IGFBP-2 and -3 responses to exercise. Hormones were evaluated in six trained male subjects before (- 30, - 15, 0), during (+ 15) and after (+ 30, + 45, + 60, + 90 min) a thirty-minutes treadmill exercise (60 % V˙O2max), both after a single administration of a somatostatin analog (i.e., octreotide, 0.1 mg sc) and after saline. The same evaluations were performed without exercise with similar treatments. The results showed that: 1) octreotide significantly inhibited the GH response to exercise, 2) exercise increased IGFBP-3 concentration (+ 37.4 % at + 90, p < 0.05), whereas no modification of IGFBP-2 and of IGF-I/IGFBP-2 and IGFBP-3/IGFBP-2 ratios were observed, 3) octreotide amplified the IGFBP-3 increase after exercise (p < 0.01 vs. exercise, from + 30 to + 60, or octreotide alone) and, without exercise, slightly increased IGFBP-3 (+ 15 % at + 75, p < 0.05) and decreased IGF-I (- 14.8 % at + 75, p < 0.01).

We concluded that GH has a reduced role, as a stimulating factor, in the serum acute IGFBP-3 increase after exercise and that octreotide is probably able to directly amplify this response. Unfortunately, we can only speculate on the physiological pathways involved.

Key words:

GH, IGF-I, IGFBP-2, IGFBP-3, octreotide, physical exercise.

References

  • 1 Bang P, Brandt J, Degerblad M, Enberg G, Kaijser L, Thoren M, Hall K. Exercise-induced changes in insulin-like growth factors and their low molecular weight binding protein in healthy subjects and patients with growth hormone deficiency.  Eur J Clin Invest. 1990;  20 285-292
    PubMedSearch in Google Scholar
  • 2 Baxter R C. Physiological roles of the IGF binding proteins. In: Spencer EM (ed) Modern concepts of insulin-like growth factors. New York; Elsevier 1991: 371-380
    Search in Google Scholar
  • 3 Baxter R C, Hizuka N, Takano K, Holman S R, Asakawa K. Responses of insulin-like growth factor binding protein-1 (IGFBP-1) and the IGFBP-3 complex to administration of insulin-like growth factor-I.  Acta Endocrinol. 1993;  128 101-108
    PubMedSearch in Google Scholar
  • 4 Blethen S L, Daughaday W H, Weldon V V. Kinetics of the somatomedin C/insulin-like growth factor I: response to exogenous growth hormone (GH) in GH-deficient children.  J Clin Endocrinol Metab. 1982;  54 986-990
    PubMedSearch in Google Scholar
  • 5 Clemmons D R. IGF binding proteins and their functions.  Mol Reprod Dev. 1993;  35 368-375
    CrossrefPubMedSearch in Google Scholar
  • 6 Cumming C. Hormones and athletic performance. In: Felip P, Baxter JD, Frohman LA (eds) Endocrinology and metabolism. New York; Mc Graw Hill 1995: 1837-1886
    Search in Google Scholar
  • 7 Deyssig R, Frish H, Bluma W F, Waldhor T. Effect of growth hormone treatment on hormonal parameters, body composition and strength in athletes.  Acta Endocrinol. 1993;  128 313-318
    PubMedSearch in Google Scholar
  • 8 Di Luigi L, Conti F G, Casini A, Guidetti L, Zezze G, Pigozzi F, Spera G, Fortunio G, Romanelli F. Growth hormone and insulin-like growth factor I responses to moderate submaximal acute physical exercise in men: effects of octreotide, a somatostatin analog, administration.  Int J Sports Med. 1997;  18 257-263
    PubMedSearch in Google Scholar
  • 9 Eliakim A, Brasel J A, Mohan S, Barstow T J, Berman N, Cooper D M. Physical fitness, endurance training, and the growth hormone-insulin-like growth factor I system in adolescent females.  J Clin Endocrinol Metab. 1996;  81 3986-3992
    CrossrefPubMedSearch in Google Scholar
  • 10 Eliakim A, Brasel J A, Mohan S, Wong W LT, Cooper D M. Increased physical activity and the growth hormone-IGF-I axis in adolescent males.  Am J Physiol. 1998;  275 R308-R314
    PubMedSearch in Google Scholar
  • 11 Eliakim A, Brasel J A, Barstow T J, Mohan S, Wong W LT, Cooper D M. Peak oxygen uptake, muscle volume, and the growth hormone-insulin-like growth factor-I axis in adolescent males.  Med Sci Sports Exerc. 1998;  30 512-517
    PubMedSearch in Google Scholar
  • 12 Ezzat S, Ren S G, Braunstein G D, Melmed S. Octreotide stimulates insulin-like growth factor-binding protein-1: a potential pituitary-independent mechanism for drug action.  J Clin Endocrinol Metab. 1992;  75 1459-1463
    CrossrefPubMedSearch in Google Scholar
  • 13 Florini J R, Ewton D Z, Coolican S T. Growth hormone and the insulin-like growth factor system in myogenesis.  Endocr Rev. 1996;  17 481-517
    CrossrefPubMedSearch in Google Scholar
  • 14 Flyvbjerg A, Jorgensen K D, Marshall S M, Orskov H. Inhibitory effect of octreotide on growth hormone-induced IGF-I generation and organ growth in hypophysectomized rats.  Am J Physiol. 1991;  260 E568-E574
    PubMedSearch in Google Scholar
  • 15 Flyvbjerg A, Schuller A G, van Neck J W, Groffen C, Orskov H, Drop S L. Stimulation of hepatic insulin-like growth factor-binding protein-1 and -3 gene expression by octreotide in rats.  J Endocrinol. 1995;  147 545-551
    PubMedSearch in Google Scholar
  • 16 Harris A G. Somatostatin and somatostatin analogs: pharmacokinetics and pharmacodynamic effects.  Gut. 1994;  35 S1-S4
    PubMedSearch in Google Scholar
  • 17 Hopkins N J, Jakeman P M, Hughes S C, Holly M P. Changes in circulating insulin-like growth factor-binding protein-1 (IGFBP-1) during prolonged exercise: effect of carbohydrate feeding.  J Clin Endocrinol Metab. 1994;  79 1887-1890
    CrossrefPubMedSearch in Google Scholar
  • 18 Istituto Nazionale della Nutrizione Umana .Società Italiana di Nutrizione Umana (eds) Livelli di assunzione giornalieri raccomandati di energia e nutrienti per la popolazione italiana (Recommended daily allowances of energy and nutrients for the Italian population). Milano; Litho DELTA 1990: 9-55
    Search in Google Scholar
  • 19 Jones J I, Clemmons D R. Insulin like growth factors and their binding proteins: biological actions.  Endocr Rev. 1995;  16 3-34
    CrossrefPubMedSearch in Google Scholar
  • 20 Jull A, Dalgaard P, Blum W F, Bang P, Hall K, Michaelsen K F, Muller J, Skakkebaek N E. Serum levels of insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) in healthy infants, children, and adolescents: the relation to IGF-I, IGF-II, IGFBP-1, IGFBP-2, age, sex, body mass index, and pubertal maturation.  J Clin Endocrinol Metab. 1995;  80 2534-2542
    CrossrefPubMedSearch in Google Scholar
  • 21 Kicman A T, Miell J P, Teale J D, Powrie J, Wood P J, Laidler P, Milligan P J, Cowan D A. Serum IGF-I and IGF binding proteins 2 and 3 as potential markers of doping with human GH.  Clin Endocrinol. 1997;  47 43-50
    CrossrefPubMedSearch in Google Scholar
  • 22 Koistinen H, Koistinen R, Selenius L, Ylikorkala O, Seppala M. Effect of marathon run on serum IGF-I and IGF-binding protein 1 and 3 levels.  J Appl Physiol. 1996;  80 760-764
    PubMedSearch in Google Scholar
  • 23 Lalou C, Binoux M. Evidence that limited proteolysis of insulin-like growth factor binding protein-3 (IGFBP-3) occurs in the normal state outside of the blood stream.  Reg Pept. 1993;  48 179-188
    CrossrefPubMedSearch in Google Scholar
  • 24 Lee P DK, Durham S K, Martinez V, Vasconez O, Powell D R, Guevara-Aguirre J. Kinetics of insulin-like growth factor (IGF) and IGF-binding protein responses to a single dose of growth hormone.  J Clin Endocrinol Metab. 1997;  82 2266-2274
    CrossrefPubMedSearch in Google Scholar
  • 25 Nguyen U N, Mougin F, Simon-Rigaud M L, Rouillon J D, Marguet P, Regnard J. Influence of exercise duration on serum insulin-like growth factor and its binding proteins in athletes.  Eur J Appl Physiol. 1998;  78 533-537
    CrossrefPubMedSearch in Google Scholar
  • 26 Rajaram S, Baylink D J, Mohan S. Insulin-like growth factor-binding protein in serum and other biological fluids: regulation and functions.  Endocr Rev. 1997;  18 801-831
    CrossrefPubMedSearch in Google Scholar
  • 27 Schally A V. Oncological applications of somatostatin analogs.  Cancer Res. 1988;  48 6977-6985
    PubMedSearch in Google Scholar
  • 28 Schwarz A J, Brasel J O, Hintz R L, Mohan S, Cooper D M. Acute effect of brief low- and high-intensity exercise on circulating insulin-like growth factor (IGF) I, II, and IGF-binding protein-3 and its proteolysis in young healthy men.  J Clin Endocrinol Metab. 1996;  81 3492-3497
    CrossrefPubMedSearch in Google Scholar
  • 29 Smith W J, Nam T J, Underwood L E, Busby W H, Celnicker A, Clemmons D R. Use of insulin-like growth factor-binding protein-2 (IGFBP-2), IGFBP-3, and IGF-I for assessing growth hormone status in short children.  J Clin Endocrinol Metab. 1993;  77 1294-1299
    CrossrefPubMedSearch in Google Scholar
  • 30 Suikkari A M, Sane T, Seppala M, Yki-Jarvinen H, Karonen S L, Koivisto V A. Prolonged exercise increases serum insulin-like growth factor-binding protein concentrations.  J Clin Endocrinol Metab. 1989;  68 141-144
    PubMedSearch in Google Scholar
  • 31 Van Beaumont W. Evaluation of hemoconcentration from hematocrit measurements.  J Appl Physiol. 1972;  32 712-713
    PubMedSearch in Google Scholar
  • 32 Vasankari T, Kujala U, Taimela S, Torma A, Irjiala K, Huhtaniemi I. Effects of a long acting somatostatin analog on pituitary, adrenal and testicular function during rest and acute exercise: unespected stimulation of testosterone secretion.  J Clin Endocrinol Metab. 1995;  80 3298-3303
    CrossrefPubMedSearch in Google Scholar
  • 33 Weltman A, Weltman J Y, Schurrer R, Evans W S, Veldhuis J D, Rogol A D. Endurance training amplifies the pulsatile release of growth hormone: effect of training intensity.  J Appl Physiol. 1992;  72 2188-2196
    PubMedSearch in Google Scholar
  • 34 Young S CJ, Smith-Banks A, Underwood L E, Clemmons D R. Effects of recombinant IGF-I and GH treatment upon serum IGF binding proteins in calorically restricted adults.  J Clin Endocrinol Metab. 1992;  75 603-608
    CrossrefPubMedSearch in Google Scholar

Prof. L. Di Luigi, M.D.

Laboratory of Endocrine ResearchUniversity Institute of Motor Sciences/IUSM

Piazza Lauro de Bosis, 1500194 RomeItaly

Phone: Phone:+ 39 06 36095543

Fax: Fax:+ 39 06 3613065

Email: E-mail:iusm.endocrinol@uni.net