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Planta Med 2001; 67(8): 778-780
DOI: 10.1055/s-2001-18346
Letter

© Georg Thieme Verlag Stuttgart · New York

A Reversed Phase High Performance Liquid Chromatography Method for the Analysis of Boswellic Acids in Boswellia serrata

Markus Ganzera1 , Ikhlas A. Khan1, 2,*
  • 1 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, Mississippi, USA
  • 2 Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, Mississippi, USA
Further Information

Publication History

November 3, 2000

January 21, 2001

Publication Date:
09 November 2001 (online)

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Abstract

An HPLC method for the separation of boswellic acids, the active constituents in Boswellia serrata resin has been developed. The first accurate determination of 6 individual acids was possible in the resin as well as in multi-component preparations. By using an acidic mobile phase, raised temperature and a 4 μm Synergi MAX-RP 80 Å column the acids could be detected at levels as low as 0.9 μg/ml. The study of market products revealed significant variations in the content of these pharmacologically active compounds in commercial samples.

References

  • 1 Kapoor L D. CRC Handbook of Ayurvedic Medicinal Plants. CRC Press Bocca Raton; 1990: 83
  • 2 Nadkarni K M. Indian Materia Medica. Popular Prakashan Bombay; 1954: 211-2
  • 3 Leung A, Foster S. Encyclopedia of Common Natural Ingredients Used in Food, Drugs and Cosmetics. John Wiley & Sons New York; 1996: 389-91
  • 4 Safayhi H, Boden S, Schweitzer S, Ammon H. Concentration-dependent potentiating and inhibitory effects of Boswellia extracts on 5-lipoxygenase product formation in stimulated PMNL.  Planta Medica. 2000;  66 110-3
    Thieme ConnectPubMedSearch in Google Scholar
  • 5 Shao Y, Ho C T, Chin C K, Badmaev V, Ma W, Huang M T. Inhibitory activity of boswellic acids from Boswellia serrata against human leukemia HL-60 Cells in culture.  Planta Medica. 1998;  64 328-31
    PubMedSearch in Google Scholar
  • 6 Knaus U, Wagner H. Effects of boswellic acids of Boswellia serrata and other triterpenic acids on the complement system.  Phytomedicine. 1996;  3 77-80
    PubMedSearch in Google Scholar
  • 7 Hänsel R, Sticher O, Steinegger E. Pharmakognosie - Phytopharmazie. Springer Berlin; 1999: 521-2
  • 8 Hahn-Deinstrop E, Koch A, Müller M. Guidelines for the assessment of the traditional herbal medicine olibanum by application of HPTLC and DESAGA ProViDoc video documentation.  J. Planar Chromat.. 1998;  11 404-10
    PubMedSearch in Google Scholar
  • 9 Gupta V, Atal C K, D’Souza P, Nagavi B G. Estimation of different constituents of total triterpene acids from salai guggal.  Indian Drugs. 1985;  22 222-4
    PubMedSearch in Google Scholar

Dr. Ikhlas A. Khan

NCNPR

University of Mississippi

University

MS 38677

USA

Phone: +1-662-915-7821

Fax: +1-662-915-7989

Email: rikhan@olemiss.edu