Download PDF
Planta Med 2001; 67(8): 778-780
DOI: 10.1055/s-2001-18346
Letter

© Georg Thieme Verlag Stuttgart · New York

A Reversed Phase High Performance Liquid Chromatography Method for the Analysis of Boswellic Acids in Boswellia serrata

Markus Ganzera1 , Ikhlas A. Khan1, 2,*
  • 1 National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, Mississippi, USA
  • 2 Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, Mississippi, USA
Further Information

Publication History

November 3, 2000

January 21, 2001

Publication Date:
09 November 2001 (online)

Also available at
   Permissions and Reprints
Preview

Abstract

An HPLC method for the separation of boswellic acids, the active constituents in Boswellia serrata resin has been developed. The first accurate determination of 6 individual acids was possible in the resin as well as in multi-component preparations. By using an acidic mobile phase, raised temperature and a 4 μm Synergi MAX-RP 80 Å column the acids could be detected at levels as low as 0.9 μg/ml. The study of market products revealed significant variations in the content of these pharmacologically active compounds in commercial samples.

References

  • 1 Kapoor L D. CRC Handbook of Ayurvedic Medicinal Plants. CRC Press Bocca Raton; 1990: 83
  • 2 Nadkarni K M. Indian Materia Medica. Popular Prakashan Bombay; 1954: 211-2
  • 3 Leung A, Foster S. Encyclopedia of Common Natural Ingredients Used in Food, Drugs and Cosmetics. John Wiley & Sons New York; 1996: 389-91
  • 4 Safayhi H, Boden S, Schweitzer S, Ammon H. Concentration-dependent potentiating and inhibitory effects of Boswellia extracts on 5-lipoxygenase product formation in stimulated PMNL.  Planta Medica. 2000;  66 110-3
    Thieme ConnectPubMedSearch in Google Scholar
  • 5 Shao Y, Ho C T, Chin C K, Badmaev V, Ma W, Huang M T. Inhibitory activity of boswellic acids from Boswellia serrata against human leukemia HL-60 Cells in culture.  Planta Medica. 1998;  64 328-31
    PubMedSearch in Google Scholar
  • 6 Knaus U, Wagner H. Effects of boswellic acids of Boswellia serrata and other triterpenic acids on the complement system.  Phytomedicine. 1996;  3 77-80
    PubMedSearch in Google Scholar
  • 7 Hänsel R, Sticher O, Steinegger E. Pharmakognosie - Phytopharmazie. Springer Berlin; 1999: 521-2
  • 8 Hahn-Deinstrop E, Koch A, Müller M. Guidelines for the assessment of the traditional herbal medicine olibanum by application of HPTLC and DESAGA ProViDoc video documentation.  J. Planar Chromat.. 1998;  11 404-10
    PubMedSearch in Google Scholar
  • 9 Gupta V, Atal C K, D’Souza P, Nagavi B G. Estimation of different constituents of total triterpene acids from salai guggal.  Indian Drugs. 1985;  22 222-4
    PubMedSearch in Google Scholar

Dr. Ikhlas A. Khan

NCNPR

University of Mississippi

University

MS 38677

USA

Phone: +1-662-915-7821

Fax: +1-662-915-7989

Email: rikhan@olemiss.edu