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Plant Biol (Stuttg) 2002; 4(1): 13-21
DOI: 10.1055/s-2002-20431
Original Paper
Georg Thieme Verlag Stuttgart ·New York

On the Cytochemistry of Cell Wall Formation in Poplar Trees

C. Grünwald 1 , K. Ruel 2 , Y. S. Kim 3 , U. Schmitt 1
  • 1 University of Hamburg and Federal Research Centre for Forestry and Forest Products, Institute for Wood Biology, Hamburg, Germany
  • 2 Centre de Recherches sur les Macromolecules Vegetales (CERMAV), Grenoble cedex 9, France
  • 3 Chonnam National University, Department of Forest Products and Technology Kwangju 500-757, Korea
Further Information

Publication History

July 12, 2001

October 25, 2001

Publication Date:
28 February 2002 (online)

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Abstract

The ultrastructure of cell walls and the mechanisms of cell wall formation are still not fully understood. The objective of our study was therefore to obtain additional fine structural details on the deposition of cell wall components during the differentiation of xylem cells in hybrid aspen (Populus tremula L. × P. tremuloides Michx.) we used as a model tree. At the electron microscope level, PATAg staining revealed a successive deposition of polysaccharides with increasing distance from the cambium. Staining with potassium permanganate and UV microspectrophotometry showed that the cell walls were lignified, with some delay to the deposition of polysaccharides. Immunogold labelling of three lignin types in developing cell walls varied with progressive deposition of cell wall layers. Condensed lignin subunits were localized in corners of cells adjacent to the cambium prior to S1 formation, whereas non-condensed lignin subunits became labelled only in later stages - in secondary walls near cell corners and simultaneously with the completion of S1 formation. As S2 polysaccharide deposition progressed, the labelling extended towards the lumen. Labelling of peroxidases revealed their presence in cell corner regions of young xylem cells, still lacking a secondary wall, implying that peroxidases are incorporated into the developing cell wall at early developmental stages. A weak labelling of middle lamella regions and secondary walls could also be seen at later stages. The results are discussed in relation to current knowledge on the succession of polysaccharide and lignin deposition in woody cell walls.

Key words

Populus - wood formation - lignification - electron microscopy - immunogold labelling - UV microspectrophotometry

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C. Grünwald

Universität Hamburg
Ordinariat für Holzbiologie

Leuschnerstr. 91
21031 Hamburg
Germany

Email: gruenwald@holz.uni-hamburg.de

Section Editor: A. M. C. Emons