Download PDF
Plant Biol (Stuttg) 2002; 4(1): 77-85
DOI: 10.1055/s-2002-20439
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Lipoxygenase Pathway and Membrane Permeability and Composition during Storage of Potato Tubers (Solanum tuberosum L. cv Bintje and Désirée) in Different Conditions

M.-L. Fauconnier 1 , J. Rojas-Beltrán 2 , J. Delcarte 1 , F. Dejaeghere 2 , M. Marlier 1 , P. du Jardin 2
  • 1 Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Gembloux, Belgium
  • 2 Unité de Biologie Végétale, Faculté Universitaire des Sciences Agronomiques de Gembloux, Gembloux, Belgium
Further Information

Publication History

August 15, 2001

November 13, 2001

Publication Date:
28 February 2002 (online)

   Permissions and Reprints

Abstract

Potato tubers (Solanum tuberosum L. cv Bintje and Désirée) were stored for 12 months under three different storage conditions: 4 °C, 20 °C with sprout inhibitor and 20 °C without sprout inhibitor. Independent of the storage conditions, our results show that the increase of membrane permeability, as revealed by electrolyte leakage, is not correlated with the lipid saturation status. Moreover, there is no simple correlation between cold sweetening and membrane permeability or lipid saturation status. During storage at 20 °C without sprout inhibitor, the increase in membrane permeability is inversely correlated to sucrose accumulation, but this is not the case when tubers were stored with sprout inhibitors. Lipoxygenase (LOX) is often proposed as responsible for peroxidative damage to membrane lipids. The gradual peroxidation resulting in double bond index decrease is regarded as a cause of senescence sweetening. Our results revealed that the role of LOX in aging and senescence of potato tubers is far from clear. LOX activity and gene expression are not correlated with the fatty acids composition of the membrane. Moreover, LOX activity and fatty acid hydroperoxide content are low in older tubers, whatever the storage conditions or the varieties. On the basis of our results, the correlation between sugar accumulation (low temperature and senescence sweetening) and peroxidative damage occurring during storage of potato tubers is discussed.

Abbreviations

CIPC: ispropyl N-(3-chlorophenyl)carbamate

DBI: double bond index

GC: gas chromatography

LOX: lipoxygenase

MDA: malondialdehyde

ROOH: fatty acid hydroperoxides

Key words

Fatty acid hydroperoxides - lipoxygenase - membrane integrity - potato tubers - Solanum tuberosum L. - storage

References

  • 01 Siedow,  J. N.. (1991);  Plant lipoxygenase: structure and function.  Ann. Rev. Plant Physiol. Plant Mol. Biol.. 42 145-158
    Search in Google Scholar
  • 02 Delcarte,  J.,, Fauconnier,  M. L.,, Hoyaux,  P.,, Jacques,  P.,, Thonart,  P.,, and Marlier,  M.. (2000);  Revue bibliographique: l'hydroperoxyde lyase.  Biotechnol. Agron. Soc. Environ.. 4 157-167
    Search in Google Scholar
  • 03 Blée,  E.. (1998);  Phytooxylipins and plant defense reactions.  Prog. Lipid Res.. 37 33-72
    Search in Google Scholar
  • 04 Galliard,  T., and Phillips,  D.. (1972);  The enzymic conversion of linoleic acid into 9-(nona1′,3′-dienoxy)non-8-enoic acid, a novel unsaturated ether derivative isolated from homogenates of Solanum tuberosum L. tubers.  Biochem J.. 129 743-753
    Search in Google Scholar
  • 05 Hoyaux,  P.,, Fauconnier,  M. L.,, du Jardin,  P.,, and Marlier,  M.. (2001);  La divinyl éther synthase de plantes.  Biotechnol. Agron. Soc. Environ.. 5 79-84
    Search in Google Scholar
  • 06 Fidantsef,  A. L., and Bostock,  R M.. (1998);  Characterization of potato tuber lipoxygenase cDNAs and lipoxygenase expression in potato tubers and leaves.  Physiol. Plant.. 102 257-271
    Search in Google Scholar
  • 07 Kolomiets,  M. V.,, Chen,  H.,, Gladon,  R. J.,, Braun,  E. J.,, and Hannapel,  D. J.. (2000);  A leaf lipoxygenase of potato induced specifically by pathogen infection.  Plant Physiol.. 124 1121-1130
    Search in Google Scholar
  • 08 Hildebrand,  D. F.,, Rodriguez,  J. G.,, Legg,  C. S.,, Brown,  G. C.,, and Bookjans,  G.. (1989);  The effects of wounding and mite infestation on soybean leaf lipoxygenase levels.  Z. Naturforsh.. 44 655-659
    Search in Google Scholar
  • 09 Bell,  E., and Mullet,  J. E.. (1991);  Lipoxygenase gene expression is modulated in plants by water deficit, wounding and methyl jasmonate.  Mol. Gen. Genet.. 230 456-462
    Search in Google Scholar
  • 10 Geerts,  A.,, Feltkamp,  D.,, and Rosahl,  S.. (1994);  Expression of lipoxygenase in wounded tubers of Solanum tuberosum L.  Plant Physiol.. 105 269-277
    CrossrefPubMedSearch in Google Scholar
  • 11 Royo,  J.,, Vancanneyt,  G.,, Perez,  A. G.,, Sanz,  C.,, Stormann,  K.,, Rosahl,  S.,, and Sanchez-Serrano,  J. J.. (1996);  Characterization of three potato lipoxygenases with distinct enzymatic activities and different organ-specific and wound-regulated expression patterns.  J. Biol. Chem.. 271 21012-21019
    CrossrefPubMedSearch in Google Scholar
  • 12 Fukuchi-Mizutani,  M.,, Ishiguro,  K.,, Nakayam,  T.,, Utsunomiya,  Y.,, Tanaka,  Y.,, Kusumi,  T.,, and Ueda,  T.. (2000);  Molecular and functional characterization of a rose lipoxygenase cDNA related to flower senescence.  Plant Sci.. 160 129-137
    CrossrefPubMedSearch in Google Scholar
  • 13 Panavas,  T., and Rubinstein,  B.. (1998);  Oxidative events during programmed cell death in daylily (Hemerocallis hybrid) petals.  Plant Sc.. 133 125-138
    PubMedSearch in Google Scholar
  • 14 Rouet-Mayer,  M. A.,, Bureau,  J. M.,, and Laurière,  C.. (1992);  Identification and characterization of lipoxygenase isoforms in senescing carnation petals.  Plant Physiol.. 98 971-978
    PubMedSearch in Google Scholar
  • 15 Grossman,  S., and Leshem,  Y. Y.. (1978);  Lowering of endogenous lipoxygenase activity in Pisum sativum foliage by cytokinin as related to senescence.  Plant Physiol.. 43 359-362
    PubMedSearch in Google Scholar
  • 16 Sung,  J. M., and Chiu,  C. C.. (1995);  Lipid peroxide-scavenging enzymes of naturally aged soybean seed.  Plant Sci.. 110 45-52
    CrossrefPubMedSearch in Google Scholar
  • 17 Peterman,  T. K., and Siedow,  J. N.. (1985);  Behavior of lipoxygenase during establishment, senescence, rejuvenation of soybean cotyledons.  Plant Physiol.. 78 690-695
    PubMedSearch in Google Scholar
  • 18 Kar,  M., and Feierabend,  J.. (1984);  Metabolism of activated oxygen in detached wheat and rye leaves and its relevance to the initiation of senescence.  Planta. 160 385-391
    CrossrefPubMedSearch in Google Scholar
  • 19 Van Kempen,  P.,, Le Corre,  P.,, and Bedin,  P.. (1996) Phytotechnie. La pomme de terre. Rouselle, P., Robert, Y., and Crosnier, J. C., eds. Paris; INRA pp. 363-414
    Search in Google Scholar
  • 20 Hertog,  M. L. A. T. M.,, Tijskens,  L. M. M.,, and Hak,  P. S.. (1997);  The effects of temperature and senescence on the accumulation of reducing sugar during storage of potato (Solanum tuberosum L.) tubers: a mathematical model.  Postharvest Biol. Tech.. 10 67-79
    PubMedSearch in Google Scholar
  • 21 Kumar,  G. N. M., and Knowles,  N. R.. (1993);  Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum L.) seed-tubers.  Plant Physiol.. 102 115-124
    PubMedSearch in Google Scholar
  • 22 Spychalla,  J. P., and Desborough,  S. L.. (1990);  Fatty acids, membrane permeability and sugars of stored potato tubers.  Plant Physiol.. 94 1207-1213
    PubMedSearch in Google Scholar
  • 23 Knowles,  N. R., and Knowles,  L. O.. (1989);  Correlations between electrolyte leakage and degree of saturation of polar lipids from aged potato (Solanum tuberosum L.) tuber tissue.  Ann. Bot.. 63 331-338
    PubMedSearch in Google Scholar
  • 24 Frankel,  E. N.. (1991);  Review: recent advances in lipid oxidation.  J. Sci Food Agric.. 54 495-511
    PubMedSearch in Google Scholar
  • 25 Gravoueille,  J. M.. (1996) Utilisation pour l'alimentation humaine. La pomme de terre. Rouselle, P., Robert, Y., Crosnier, J. C., eds. Paris; INRA pp. 451-498
    Search in Google Scholar
  • 26 Gray,  J. I.. (1985) Simple chemical and physical methods for measuring flavor quality of fats and oils. Flavor chemistry of fats and oils. Min, D. B. and Smouse, T. H., eds. Champain (Il, USA); American Oil Chemists' Society pp. 223-239
    Search in Google Scholar
  • 27 De Weerd,  J. W.,, Hiller,  L. K.,, and Thornton,  R. E.. (1995);  Electrolyte leakage of aging potato tubers and its relationship with sprouting capacities.  Potato Research. 38 257-270
    PubMedSearch in Google Scholar
  • 28 Lowe,  C. R., and Thomas,  J. A.. (1996) Purification and analysis of enzyme preparations. Enzymology. Engel, P. C., ed. San Diego (CA, USA); Academic Press pp. 9-70
    Search in Google Scholar
  • 29 Fauconnier,  M. L.,, Perez,  A. G.,, Sanz,  C.,, and Marlier,  M.. (1997);  Purification and characterization of tomato leaf (Lycopersicon esculentum Mill.) hydroperoxide lyase.  J. Agric. Food Chem.. 45 4232-4236
    CrossrefPubMedSearch in Google Scholar
  • 30 Kawakami,  S.,, Mizuno,  M.,, and Tsuchida,  H.. (1999) The defense mechanism against oxidative stress generated in potato tubers during low-temperature storage. International Symposium for Plant Peroxidases. Columbus, Ohio (USA); Peroxidase 99, 17 - 21 June
    Search in Google Scholar
  • 31 Sambrook,  J.,, Fritsch,  E. F.,, and Maniatis,  T.. (1989) Molecular cloning: a laboratory manual, Ed. 2, Vol. 1. New York (USA); Cold Spring Harbor Laboratory Press
    Search in Google Scholar
  • 32 Lognay,  G.,, Marlier,  M.,, Baudart,  E.,, Severin,  M.,, and Casimir,  J.. (1988);  Contribution à l'étude du mil à chandelle (Pennisetum americanum L. Schum.).  Riv. Sostanze Grasse.. 65 291-294
    PubMedSearch in Google Scholar
  • 33 Wismer,  W. V.,, Worthing,  W. M.,, Yada,  R. Y.,, and Marangoni,  A. G.. (1998);  Membrane lipid dynamics and lipid peroxidation in the early stage of low-temperature sweetening in tubers of Solanum tuberosum. .  Physiologia Plantarum. 102 396-410
    CrossrefPubMedSearch in Google Scholar
  • 34 Griffiths,  G.,, Leverentz,  M.,, Silkowski,  H.,, Narinder,  G.,, and Sanchez-Serrano,  J. J.. (2000);  Lipid hydroperoxide levels in plant tissues.  J. Exp. Bot.. 51 1363-1370
    CrossrefPubMedSearch in Google Scholar
  • 35 Ellishèche,  P.. (1996) Aspects physiologiques de la croissance et du developpement. La pomme de terre. Rouselle, P., Robert, Y., and Crosnier, J. C., eds. Paris; INRA pp. 72-124
    Search in Google Scholar
  • 36 Lojkowska,  E., and Holubowska,  M.. (1989);  Changes of the lipid catabolism in potato tubers from cultivars differing in susceptibility to autolysis during the storage.  Potato Res.. 32 463-470
    PubMedSearch in Google Scholar
  • 37 Ye,  Z.,, Rodriguez,  R.,, Tran,  A.,, Hoang,  H.,, De Los Santos,  D.,, Brown,  S.,, and Vellanoweth,  R. L.. (2000);  The developmental transition to flowering represses ascorbate peroxidase activity and induces enzymatic lipid peroxidation in leaf tissue in Arabidopsis thaliana. .  Plant Sci.. 158 115-127
    CrossrefPubMedSearch in Google Scholar
  • 38 Leshem,  Y. Y.. (1987);  Membrane phospholipid catabolism and Ca 2+ activity control of senescence.  Plant Physiol.. 69 551-559
    PubMedSearch in Google Scholar
  • 39 Kumar,  G. N. M., and Knowles,  N. R.. (1996);  Oxidative stress results in increased sinks for metabolic energy during aging and sprouting of potato seed-tubers.  Plant Physiol.. 112 1301-1313
    PubMedSearch in Google Scholar
  • 40 Mc Kersie,  B. D., and Leshem,  Y. Y.. (1994) Oxidative stress. Stress and stress coping in cultivated plants. Dordrecht, Boston, London; Kluwer Academic Publisher pp. 15-54
    Search in Google Scholar
  • 41 Sowokinos,  J.. (1990) Stress-induced alterations in carbohydrate metabolism. The molecular and cellular biology of the potato. Vayda, M. E. and Park, W. D., ed. Oxon (UK); CAB International pp. 137-158
    Search in Google Scholar
  • 42 Hill,  L. M.,, Reimholz,  R.,, Schröder,  R.,, Nielsen,  T. H.,, and Stitt,  M.. (1996);  The onset of sucrose accumulation in cold-stored potato tubers is caused by an increased rate of sucrose synthesis and coincides with low levels of hexose-phosphates, an activation of sucrose phosphate synthase and the appearance of a new form of amylase.  Plant, Cell and Envir.. 19 1223-1237
    PubMedSearch in Google Scholar
  • 43 Davies,  H. V.,, Harris,  N.,, Viola,  R.,, Millam,  S.,, Barry,  G.,, and Stark,  D. M.. (1998) Starch biosynthesis in potato tubers expressing a non-regulated E. coli ADPglucose pyrophosphorylase. Bogensee (Germany), 2 - 6 August; 5th International Symposium on the Molecular Biology of the Potato
    Search in Google Scholar

M.-L. Fauconnier

Unité de Chimie Générale et Organique
Faculté Universitaire des Sciences Agronomiques de Gembloux

Passage des Déportés, 2
5030 Gembloux
Belgium

Email: fauconnier.ml@fsagx.ac.be

Section Editor: H. Rennenberg