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Planta Med 2002; 68(3): 232-236
DOI: 10.1055/s-2002-23127
Original Paper
Natural Product Chemistry
© Georg Thieme Verlag Stuttgart · New York

An Arabinogalactan-Protein from Cell Culture of Malva sylvestris

Birgit Classen1 , Wolfgang Blaschek1
  • 1Pharmazeutisches Institut der Christian-Albrechts-Universität Kiel, Abteilung Pharmazeutische Biologie, Kiel, Germany
Further Information

Publication History

March 9, 2001

June 30, 2001

Publication Date:
25 March 2002 (online)

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Abstract

An arabinogalactan-protein (AGP) from suspension culture medium of Malva sylvestris was isolated by precipitation with β-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of AGPs: a high amount of polysaccharide with a ratio of galactose to arabinose of 1.9 : 1, some uronic acids, and a small protein moiety with the main amino acids serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.3 × 106 Da. Linkage analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Galp residues with terminal Araf, GlcAp and Galp. Partial acid hydrolysis resulted in loss of Araf residues at the periphery of the molecule and heavily reduced its reactivity with β-glucosyl Yariv antigen.

Key words

Arabinogalactan proteins - Malva sylvestris - Malvaceae - cell culture - structural analysis - β-glucosyl Yariv reagent

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1 * Afterwards vitamins and phytohormones were added as sterilized solutions (filtration).

Dr. Birgit Classen

Pharmazeutisches Institut der Christian-Albrechts-Universität Kiel

Abteilung Pharmazeutische Biologie

Gutenbergstr. 76

24118 Kiel

Germany

Email: bclassen@pharmazie.uni-kiel.de

Fax: +49-(0)431-880-1102