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DOI: 10.1055/s-2002-23140
© Georg Thieme Verlag Stuttgart · New York
Deoxypodophyllotoxin; The Cytotoxic and Antiangiogenic Component from Pulsatilla koreana
Publication History
April 11, 2001
September 16, 2001
Publication Date:
25 March 2002 (online)
![](https://www.thieme-connect.de/media/plantamedica/200203/lookinside/thumbnails/10.1055-s-2002-23140-1.jpg)
Abstract
The petroleum ether fraction of Pulsatilla koreana (Ranunculaceae) was found to have an inhibitory effect on the tube-like formation of human umbilical venous endothelial (HUVE) cells and strong cytotoxic activity against five tumor cell lines. The active component isolated from the fraction was deoxypodophyllotoxin (DPT). The cytotoxic activity against the tumor cells comprising the A549, SK-OV-3, SK-MEL-2, HCT15, and B16F10 cell lines, expressed as ED50, ranged from 6 to 18 ng/ml. 3 ng/ml of DPT, a concentration considerably below the cytotoxic concentration, completely inhibited the tube-like formation of HUVE cells. Furthermore, DPT exhibited an inhibition ratio of 60 % on BDF1 mice bearing Lewis lung carcinoma cells. The inhibitory effect on the tube-like formation was suggested to play an important role in antitumor activity.
References
- 1 Hsu H Y, Chen Y P, Shen S J, Hsu C S, Chen C C, Chang H C. Oriental Materia Medica. Oriental Healing Art Institute 1986: 226-7
- 2 Zhang X Q, Liu A R, Xu L X. Determination of ranunculin in Pulsatilla chinensis and synthetic ranunculin by reversed phase HPLC. Yao Hsueh Hsueh Pao. 1990; 25 932-5
- 3 Ye W C, Ou B X, Ji N N, Zhao S X, Ye T, McKervey M A, Stevenson P. Patensin, a saponin from Pulsatilla patens var. multifida . Phytochemistry. 1995; 39 937-9
- 4 Ye W C, Nine N J, Shou X Z, Jing H L, Tao Y, Meckerry M A, Stevenson P. Triterpenoids from Pulsatilla chinensis . Phytochemistry. 1996; 42 799-802
- 5 Vonderbank H. Antibiotics from plants and animals. Pharmazie. 1950; 5 210-7
- 6 Li R Z, Ji X J. The cytotoxicity and action mechanism of ranunculin in vitro . Yao Hsueh Hsueh Pao. 1993; 28 326-31
- 7 Boguchi D E, Charlton S L. An asymmetric synthesis of (-)-deoxypodophyllotoxin. J. Org. Chem.. 1995; 60 588-93
- 8 Wen C Y, Nine N J, Shou X Z, Jing H L, Mckervey M A, Stevenson P. Triterpenoids from Pulsatilla chinensis . Phytochemistry. 1996; 42 799-802
- 9 Skehan P, Storeng R, Scudiero D, Monka A, McMahon J, Vistica D, Warren J T, Bokesch H, Kenny S, Boyd M R. New calorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer Inst.. 1990; 82 1107-12
- 10 Yasumasa I, Yukihide I, Kazuhiro T, Kiyotaka O, Yoichi S. A quantitative assay using basement membrane extract to study tumor angiogenesis in vivo . Int. J. Cancer,. 1996; 67 148-52
- 11 Teruhiro U, Jiro S, Yoshikazu S, Kumio A, Yuji Y. Antitumor activity of a novel podophyllotoxin derivatives (TOP-53) against lung cancer and lung metastatic cancer. Cancer Res.. 1996; 56 2809-14
- 12 Bianchi E, Caldwell M E, Cole J R. Antitumor agents from Bursera microphylla (Burseraceae) I. Isolation and characterization of deoxypodophyllotoxin. J. Pharm. Sci.. 1968; 57 696-7
- 13 Noguchi T, Kawanami M. Studies on the constitutent of Anthricus sylvestris . Hoffm. Yakugaku Zasshi. 1940; 60 2809-14
- 14 San F A, Gordaliza M, Miguel J M, Castro M A, Garcia M D, Ruiz P. Antineoplastic and antiviral activities of some cyclolignans. Planta Med.. 1993; 59 246-9
- 15 Tomita M, Lu S T, Chen Y Y. Alkaloids of Hernandia ovigera L. Yakugaku Zasshi. 1966; 86 763-6
- 16 Yoshihiro M, Minpei K, Tomoki A, Yutaka S. Triterpene saponins and lignans from the roots of Pulsatilla chinensis and their cytotoxic activity against HL-60 cells. J. Nat. Prod.. 1999; 62 1279-83
- 17 Terada T, Fujimoto K, Nomura M, Kobunai T, Takeda S, Wierzbi K, Yamada Y, Yamaguchi H. Antitumor agent. I. DNA topoisomerase II inhibitory activity and the structural relationship of podophyllotoxin derivatives as antitumor agents. Chem. Pharm. Bull.. 1992; 40 2720-7
Byung-Zun Ahn
College of Pharmacy
Chungnam National University
Taejon 305-764 Korea
Fax: +82 42 823-6566
Email: ahnbj@cnu.ac.kr