Effect of Drought Stress on the Yield and Composition of Volatile Oils of Drought-Tolerant and Non-Drought-Tolerant Clones of Tagetes minuta

Abstract

A drought-tolerant clone of Tagetes minuta L. (Asteraceae) was selected in vitro on a medium containing 60 mM mannitol. In the greenhouse, a decrease in soil field capacity (FC) from 100 to 40 % reduced oil yield by 49, 71 and 71 % for drought-tolerant, non-drought tolerant cloned and seed-grown plants, respectively. In soil at 40 % FC, the drought-tolerant clone had the highest leaf dry weight and oil yield. At 100 % FC, there was no significant difference in leaf dry weight, but the drought-tolerant clone had a significantly higher percentage oil content and yield than seed-grown plants. The main components of the oil were monoterpenes of which trans- and cis-tagetone together were 52.3 % - 64.2 %. Drought significantly altered the content of some oil components.

References

• 1 Vasudevan P, Suman K, Satyawati S. A multipurpose plant.  Bioresource Technology. 1997;  62 29-35
• 2 Chalchat J C, Garry R, Muhayimana A. Essential oil of Tagetes minuta from Rwanda and France: Chemical composition according to harvesting location, growth stage and part of plant extracted.  Journal of Essential Oil Research. 1995;  7 375-86
• 3 Daghero J, Mattea M, Reverchon E, Della Porta G, Senatore F. Isolation of Tagetes minuta L. oil using supercritical CO₂ extraction.  Acta Horticulturae. 1999;  503 21-6
• 4 Héthélyi E, Dános B, Tétényi P. GC-MS analysis of the essential oils of four Tagetes species and the anti-microbial activity of Tagetes minuta .  Flavour and Fragrance Journal. 1986;  1 169-73
• 5 Bikram S, Sood R P, Singh V. Chemical composition of Tagetes minuta L. oil from Himachal Pradesh (India).  Journal of Essential Oil Research. 1992;  4 525-6
• 6 Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures.  Physiologia Plantarum. 1962;  15 473-97
• 7 Mohamed M A-H, Harris P JC, Henderson J. An efficient in vitro regeneration protocol for Tagetes minuta .  Plant Cell Tissue and Organ Culture. 1999;  55 211-5
• 8 Mohamed M A-H, Harris P JC, Henderson J. In vitro selection and characterisation of a drought tolerant clone of Tagetes minuta .  Plant Science. 2000;  159 213-22
• 9 Tuomela K. Leaf water relations in six provenances of Eucalyptus microtheca: A greenhouse experiment.  Forest Ecology and Management. 1997;  92 1-10
• 10 Zar J H. Biostatisical Analysis. 3rd Edn New Jersey; Prentice Hall 1996