Effect of the Essential Oil from the Flowers of Magnolia sieboldii on the Lipopolysaccharide-Induced Production of Nitric Oxide and Prostaglandin E₂ by Rat Peritoneal Macrophages

Abstract

The essential oil from the flowers of Magnolia sieboldii was tested for its effects on lipopolysaccharide (LPS)-induced production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) by rat peritoneal macrophages. It was shown to induce the production of NO and PGE₂ in a concentration-dependent manner (3 - 30 μg/ml). Gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS) led to the identification of sixty compounds, of which β-elemene (18.0 %), α-terpinene (14.83 %) and β-myrcene (12.72 %) were the major constituents. Among these three compounds, α-terpinene was found to be the most effective one with inhibitory activity on NO and PGE₂ production by LPS-stimulated rat peritoneal macrophages.

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