Planta Med 2002; 68(6): 572-574
DOI: 10.1055/s-2002-32561
Letter
© Georg Thieme Verlag Stuttgart · New York

Simultaneous Analysis of Alkamides and Caffeic Acid Derivatives for the Identification of Echinacea purpurea, Echinacea angustifolia, Echinacea pallida and Parthenium integrifolium Roots

Magali Laasonen1, 2 , Tero Wennberg1 , Tuulikki Harmia-Pulkkinen2 , Heikki Vuorela1
  • 1Department of Pharmacy, Division of Pharmacognosy, University of Helsinki, Finland
  • 2Pharmia Oy, Helsinki, Finland
Further Information

Publication History

September 20, 2001

January 20, 2002

Publication Date:
01 July 2002 (online)

Abstract

A reversed-phase HPLC method was developed using a computer simulation program for the identification of dried roots of Echinacea purpurea, E. angustifolia, E. pallida and Parthenium integrifolium. Hydrophilic and lipophilic compounds were analysed simultaneously leading to a two-fold decrease in analysis time compared to traditional HPLC methods.

References

  • 1 Bauer R, Khan I A, Wagner H. Nachweis einer Verfälschung von Echinacea purpurea (L.) Moench mit Parthenium integrifolium L.  Deutsche Apotheker Zeitung. 1987;  127 1325-30
  • 2 Bauer R, Khan I A, Wagner H. TLC and HPLC Analysis of Echinacea pallida and E. angustifolia roots.  Planta Medica. 1988;  54 426-30
  • 3 Heubl G R, Bauer R, Wagner H. Morphologische und Anatomische Studien an Echinacea purpurea, E. angustifolia, E. pallida und Parthenium integrifolium .  Scientia Pharmaceutica. 1988;  56 145-60
  • 4 Bauer R, Khan I A, Wagner H. Echinacea-Drogen. Standardisierung mittels HPLC und DC.  Deutsche Apotheker Zeitung. 1986;  126 1065-70
  • 5 Pietta P, Mauri P, Bauer R. MEKC Analysis of different Echinacea species.  Planta Medica. 1998;  64 649-52
  • 6 Baum B R, Mechanda S, Livesey J F, Binns S E, Arnason J T. Predicting quantitative phytochemical markers in single Echinacea plants or clones from their DNA fingerprints.  Phytochemistry. 2001;  56 543-9
  • 7 Laasonen M, Harmia-Pulkkinen T, Simard C L, Michiels E, Räsänen M, Vuorela H. Fast identification of Echinacea purpurea dried roots using near infrared spectroscopy. Analytical Chemistry 2001; in press
  • 8 Bauer R, Reminger P. TLC and HPLC analysis of alkamides in Echinacea drugs.  Planta Medica. 1989;  55 367-71
  • 9 Bergeron C, Livesey J F, Awang D VC, Arnason J T, Rana J, Baum B R, Letchamo W. A Quantitative HPLC method for the quality assurance of Echinacea products on the North American market.  Phytochemical Analysis. 2000;  11 207-15
  • 10 Perry N B, Van Klink J W, Burgess E J, Parmenter G A. Alkamide levels in Echinacea purpurea: effects of processing, drying and storage.  Planta Medica. 2000;  66 54-6
  • 11 Bauer R, Wagner H. Echinacea species as potential immunostimulatory drugs. Wagner H, Farnsworth NR editors In Economic and Medicinal Plant Research. New York; Academic Press 1991: 259-60
  • 12 Perry N B, Burgess E J, Leanne Glennie V. Echinacea standardisation: analytical methods for phenolic compounds and typical levels in medicinal species.  Journal of Agriculture and Food Chemistry. 2001;  49 1702-6
  • 13 Nüsslein B, Kurzmann M, Bauer R, Kreis W. Enzymatic degradation of cichoric acid in Echinacea purpurea preparations.  Journal of Natural Product. 2000;  63 1615-8
  • 14 Schenk R, Franke R. Content of Echinacoside in Echinacea Roots.  Beiträge zur Züchtungsforschung. 1996;  2 64-7
  • 15 Sloley B D, Urichuk L J, Tywin C, Coutts R T, Pang P KT, Shan J. Comparison of chemical components and antioxidant capacity of different Echinacea Species.  Journal of Pharmacy and Pharmacology. 2001;  53 49-57

Prof. Dr. Heikki Vuorela

Department of Pharmacy

Division of Pharmacognosy

P.O. Box 56

FIN-00014 University of Helsinki

Finland

Email: heikki.vuorela@helsinki.fi

Fax: +358 9 191 59578