Download PDF
Planta Med 2002; 68(7): 647-649
DOI: 10.1055/s-2002-32903
Letter
© Georg Thieme Verlag Stuttgart · New York

ent-Trachyloban-3β-ol, a New Cytotoxic Diterpene from Croton zambesicus

S. Block1 , C. Stévigny1 , M.-C. De Pauw-Gillet2 , E. de Hoffmann3 , G. Llabrès4 , V. Adjakidjé5 , J. Quetin-Leclercq1
  • 1Laboratoire de Pharmacognosie, Unité d’Analyse Chimique et Physico-Chimique des Médicaments, Université Catholique de Louvain, UCL 72.30-CHAM, Bruxelles, Belgium
  • 2CRCE, Histologie-Cytologie Université de Liège, Liège, Belgium
  • 3Laboratoire de Spectrométrie de Masse, Université Catholique de Louvain, Louvain-la-Neuve, Belgium
  • 4Département de Physique Expérimentale, Université de Liège, Liège, Belgium
  • 5Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Nationale du Bénin, Abomey-Calavy, Bénin
Further Information

Publication History

Received: October 22, 2001

Accepted: February 24, 2002

Publication Date:
22 July 2002 (online)

Also available at
    Permissions and Reprints

Abstract

The dichloromethane extract of leaves of Croton zambesicus (Euphorbiaceae) showing in vitro cytotoxicity against human cervix carcinoma cells was investigated in order to identify its active compounds. A bio-guided fractionation by HSCCC followed by MPLC led us to isolate a trachylobane diterpene, ent-trachyloban-3β-ol, with cytotoxic properties (IC50 on HeLa cells = 7.3 μg/ml) . This is the first report on the cytotoxicity of a trachylobane diterpene.

References

  • 1 Adjanohoun E J, Adjakidje V, de Souza S. Contribution aux études ethnobotaniques et floristiques en République Populaire du Bénin,. Vol. 1: p 245 1989 Agence de coopération culturelle et technique
    Search in Google Scholar
  • 2 Ngadjui B T, Folefoc G G, Keumedjio F, Dongo E, Sondengam B L, Connolly J D. Crotonadiol, a labdane diterpenoid from the stem bark of Croton zambesicus .  Phytochemistry. 1999;  51 171-4
    CrossrefPubMedSearch in Google Scholar
  • 3 Arnone A, Mondelli R. 13C-NMR spectroscopy of natural substances IV - 13C-NMR studies of trachylobane diterpenes: complete carbon assignment.  Organic Magnetic Resonance. 1979;  12 429-31
    CrossrefPubMedSearch in Google Scholar
  • 4 Kapingu M C, Guillaume D, Mbwambo Z H, Moshi M J, Uliso F C, Mahunnah R LA. Diterpenoids from the roots of Croton macrostachys .  Phytochemistry. 2000;  54 767-70
    CrossrefPubMedSearch in Google Scholar
  • 5 Leong Y W, Harrison L J. Ent-Trachylobane diterpenoids from the liverwort Mastigophora diclados .  Phytochemistry. 1997;  45 1457-9
    CrossrefPubMedSearch in Google Scholar
  • 6 Fraga B M. The trachylobane diterpenes.  Phytochemical analysis. 1994;  5 49-56
    PubMedSearch in Google Scholar
  • 7 Harrigan G G, da S.Bolzani V, Gunatilaka A AL, Kingston D GI. Kaurane and trachylobane diterpenes from Xylopia aethiopica .  Phytochemistry. 1994;  36 109-13
    CrossrefPubMedSearch in Google Scholar
  • 8 Moraes M PL, Roque N F. Diterpenes from the fruit of Xylopia aromatica .  Phytochemistry. 1988;  27 3205-8
    CrossrefPubMedSearch in Google Scholar
  • 9 Huang R -L, Chen C -C, Huang Y -L, Ou J -C, Hu C -P, Chen C-F, Chang C. Anti-tumor effects of d-dicentrine from the root of Lindera megaphylla .  Planta Medica. 1998;  64 212-5
    Thieme ConnectPubMedSearch in Google Scholar

Sébastien Block

Laboratoire de pharmacognosie

Unité CHAM

UCL 72.30

Avenue E. Mounier 72

1200 Bruxelles

Belgium

Email: sebastien.block@cham.ucl.ac.be

Fax: +32 2 764.72.53