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DOI: 10.1055/s-2002-35666
© Georg Thieme Verlag Stuttgart · New York
Antioxidant, Free Radical Scavenging and Anti-Inflammatory Effects of Aloesin Derivatives in Aloe vera
Publication History
Received: February 8, 2002
Accepted: June 15, 2002
Publication Date:
26 November 2002 (online)

Abstract
Antioxidant components in Aloe vera were examined for lipid peroxidation using rat liver microsomal and mitochondrial enzymes. Among the aloesin derivatives examined, isorabaichromone showed a potent antioxidative activity. The DPPH radical and superoxide anion scavenging activities were determined. As one of the most potent components, isorabaichromone together with feruloylaloesin and p-coumaroylaloesin showed potent DPPH radical and superoxide anion scavenging activities. Electron spin resonance (ESR) using the spin trapping method suggested that the potent superoxide anion scavenging activity of isorabaichromone may have been due to its caffeoyl group. As A. vera has long been used to promote wound healing, the inhibitory effects of aloesin derivatives for cyclooxygenase (Cox)-2 and thromboxane (Tx) A2 synthase were examined and the participation of p-coumaroyl and feruloyl ester groups in the aloesin skeleton was demonstrated. These findings may explain, at least in part, the wound healing effects of A.vera.
Abbreviations
ADP:adenosine diphosphate
ASA:ascorbic acid
BHT:butylated hydroxytoluene
BSA:bovine serum albumin
DMPO:5,5-dimethyl-1-pyrroline N-oxide
DPPH:1,1-diphenyl-2-picrylhydrazyl
EDTA:edetic acid
HEPES:N-(2-hydroxyethyl)-piperazine-N-2′-ethane-sulfonic acid
NADH:reduced nicotinamide adenine dinucleotide
NADPH:reduced nicotinamide adenine dinucleotide phosphate
NBT:nitroblue tetrazolium
Pg:prostaglandin
SOD:superoxide dismutase
TBA:thiobarbituric acid
TCA:trichloroacetic acid
XOD:xanthine oxidase
Key words
Aloe vera - Asphodelaceae - isorabaichromone - antioxidants - free radical - ESR - anti-inflammatory
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Prof. Akira Yagi
Faculty of Pharmacy and Pharmaceutical Sciences
Fukuyama University
Fukuyama Hiroshima 729-0292 Japan
Email: yagi@fupharm.fukuyama-u.ac.jp
Fax: +81-849/36-2024