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Horm Metab Res 2002; 34(11/12): 709-715
DOI: 10.1055/s-2002-38240
Original Basic

© Georg Thieme Verlag Stuttgart · New York

Expression of Angiotensin II Receptors Type 1 and Type 2 in Human Preadipose Cells During Differentiation

P.  Schling1
  • 1Institut für Klinische Chemie und Laboratoriumsmedizin, Klinik der Universität Regensburg, Germany
Further Information

Publication History

Received 25 April 2002

Accepted after revision 4 September 2002

Publication Date:
27 March 2003 (online)

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Abstract

Human adipose tissue expresses all the components necessary for the production of angiotensin peptides. Although local effects of angiotensin II on cells from adipose tissue are beginning to be recognised, the expression of angiotensin receptors on human preadipocytes and adipocytes is still controversial. This study addresses the issue by monitoring the mRNA levels as well as the protein production of angiotensin II receptors of type 1 and 2 (AT1 and AT2) during differentiation of primary human preadipocytes in culture and in mature adipocytes. mRNA levels of the two receptor types are inversely correlated during adipose conversion. AT1 receptor mRNA is greatly diminished within 12 days after induction of differentiation, while AT2 receptor mRNA is elevated. mRNA levels of mature adipocytes confirm this trend. The regulation is not seen as strongly on the protein level. The amount of AT2 receptor protein is increased, correlating well with the rise in specific glycerol-3-phosphate dehydrogenase activity of the cells, but the AT1 receptor protein does not vary during the whole differentiation period. As the functional role of AT2 receptors in adipose tissue is not known to date, further studies have to show if the AT1-mediated inhibitory actions on adipose conversion are downregulated in differentiating cells through decreased AT1/AT2 receptor ratio.

Key words

Adipose Tissue - Renin-Angiotensin System - AT1 - AT2 - Western Blot - RT-PCR

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Dr. Petra Schling

Institut für Klinische Chemie und Laboratoriumsmedizin · Klinik der Universität Regensburg

Franz-Josef-Strauß-Allee 11 · 93053 Regensburg · Germany ·

Phone: + 49-941-944 6207

Fax: + 49-941-944 6202

Email: petra.schling@klinik.uni-regensburg.de