Abstract
β-Galactosidase from Bacillus
circulans (EC 3.2.1.23) catalyses the transfer of galactose
units to various glucose and galactose derivatives, forming β1→4
linkages. The synthesis of several biologically relevant tri- and
tetrasaccharides {β-d-Galp-(1→4)-β-d-Galp-(1→4)-α,β-d-Glcp (2), β-d-Galp-(1→4)-α-d-Glcp-(1→4)-d-Glcp-ol/Manp-ol (4), β-d-Galp-(1→4)-α-d-Glcp-(1→6)-β-d-Fruf (6), β-d-Galp-(1→4)-α-d-Glcp-(1↔2)-β-d-Fruf (8), β-d-Galp-(1→4)-α-d-Glcp-(1↔2)-β-d-Fruf-(1↔2)-β-d-Fruf (10), β-d-Galp-(1→4)-α-d-Glcp-(1→6)-[α-d-Glcp-(1↔2)]-β-d-Fruf (12a), β-d-Galp-(1→4)-α-d-Glcp-(1↔2)-[α-d-Glcp-(1→6)]-β-d-Fruf (12b), α-d-Galp-(1→6)-[β-d-Galp-(1→4)]-α-d-Glcp-(1↔2)-β-d-Fruf (14a), β-d-Galp-(1→4)-α-d-Galp-(1→6)-α-d-Glcp-(1↔2)-β-d-Fruf (14b)} has been achieved in 11-59% yields by
application of this enzyme.
Key words
transglycosylations - β-galactosidase -
Bacillus circulans
- enzymes - tri- and tetrasaccharides
