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Planta Med 2003; 69(4): 321-326
DOI: 10.1055/s-2003-38886
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Isolation of (+)-Catechin and (-)-Epicatechin from Actinidia arguta as Bone Marrow Cell Proliferation Promoting Compounds

Fumihide Takano1 , Tomoaki Tanaka1 , Eiko Tsukamoto1 , Nobuo Yahagi1 , Shinji Fushiya1
  • 1Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku University, Aobayama, Aoba-ku, Sendai, Japan
Further Information

Publication History

Received: July 28, 2002

Accepted: January 5, 2003

Publication Date:
23 April 2003 (online)

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Abstract

The MeOH extract of stems of Actinidia arguta promoted proliferation of cultured bone marrow cells and stimulated formation of myeloid colonies from bone marrow cells. (+)-Catechin (1) and (-)-epicatechin (2) were isolated as active compounds from the MeOH extract. Compounds 1 and 2 stimulated the cell proliferation in a concentration-dependent manner in the range of 1 to 100 mg/mL. Compounds 1 and 2 also stimulated formation of myeloid colonies and enhanced the effect of interleukin-3 (IL-3) to increase the number of colony forming-units in culture (CFU-c). In an ex vivo experiment using a model mouse of decreasing bone marrow functions, orally administrated 1 (100 mg/kg/day) stimulated IL-3-induced CFU-c formation of the bone marrow cells.

Key words

Actinidia arguta - Actinidiaceae - (+)-catechin - (-)-epicatechin - bone marrow cells - proliferation - myeloid colony

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Prof. Dr. S. Fushiya

Dept. of Exp. Station for Medicinal Plant Studies

Graduate School of Pharmaceutical Sciences

Tohoku University

Aobayama

Aoba-ku

Sendai 980-8578

Japan

Email: fushiya@mail.pharm.tohoku.ac.jp

Fax: +81-22-217-6798