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DOI: 10.1055/s-2003-41115
© Georg Thieme Verlag Stuttgart · New York
Antimicrobial Constituents from Goldenseal (the Rhizomes of Hydrastis canadensis) against Selected Oral Pathogens
Publication History
Received: November 12, 2002
Accepted: March 22, 2003
Publication Date:
04 August 2003 (online)
![](https://www.thieme-connect.de/media/plantamedica/200307/lookinside/thumbnails/10.1055-s-2003-41115-1.jpg)
Abstract
Two new C-methyl flavonoids, 6,8-di-C-methylluteolin 7-methyl ether (1) and 6-C-methylluteolin 7-methyl ether (2), were isolated from a commercially available sample of the roots of Hydrastis canadensis, along with seven known compounds, berberine (3), β-hydrastine (4), canadine (5), canadaline (6), isocorypalmine (7), canadinic acid (8), and β-sitosterol 3-O-β-D-glucoside (9). The structures of the new compounds 1 and 2 were elucidated on the basis of their spectral data including 1D and 2D NMR techniques. Of these isolates, berberine (3) and, to a lesser extent, 1 and 2, showed antimicrobial activity when evaluated against the oral pathogens Streptococcus mutans and Fusobacterium nucleatum. Berberine (3) exhibited an additive antimicrobial effect when tested against S. mutans in combination with 1.
Key words
Goldenseal - Hydrastis canadensis - Ranunculaceae - C-methyl flavonoids - oral pathogens - berberine - antimicrobial activity
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Prof. A. Douglas Kinghorn
Program for Collaborative Research in the Pharmaceutical Sciences and Department of Medicinal Chemistry and Pharmacognosy
College of Pharmacy
University of Illinois at Chicago
Chicago
IL 60612
USA
Phone: +1-312-996-0914
Fax: +1-312-996-7107
Email: kinghorn@uic.edu