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Der Nuklearmediziner 2003; 26(2): 96-103
DOI: 10.1055/s-2003-41791
Gastrointestinale Diagnostik

© Georg Thieme Verlag Stuttgart · New York

Der Einsatz radioaktiv markierter Leukozyten bei entzündlichen Darmerkrankungen

The Use of Labelled Leukocytes in Inflammatory Bowel DiseaseA. M. Peters1
  • 1Department of Nuclear Medicine Addenbrooke’s Hospital Cambrigde, United Kingdom
Further Information

Publication History

Publication Date:
08 September 2003 (online)

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Zusammenfassung

Entzündliche Darmerkrankungen sind vermutlich weiterhin die am weitesten verbreitete Indikation für die Leukozytenszintigraphie. Markierte Leukozyten sind in der Diagnostik entzündlicher Darmerkrankungen anderen Methoden überlegen. Die Szintigraphie mit Tc-99m-Markierungen bleibt eins der wichtigsten nuklearmedizinischen Untersuchungsverfahren.

Abstract

Inflammatory Bowel Disease is probably still the commonest indication for imaging inflammation. In IBD no agent has been shown to be superior to labelled leukocytes. Tc-99m-labelled leukocyte scintigraphy remains as soon one of the most important investigations in nuclear medicine

Schlüsselwörter

Markierte Leukozyten - entzündliche Darmerkrankungen - Leukozytenszintigraphie

Key words

Labelled leukocytes - Inflammatory Bowel Disease - leukocyte scintigraphy

Literatur

  • 1 Saverymuttu S H, Peters A M, Hodgson H J, Chadwick V S, Lavender J P. Indium-111 autologous leucocyte scanning: comparison with radiology for imaging the colon in inflammatory bowel disease.  Br Med J. 1982;  285 255-257
    PubMedSearch in Google Scholar
  • 2 Saverymuttu S H, Peters A M, Lavender J P, Hodgson H JF, Chadwick V S. Indium-111 autologous leucocytes in inflammatory bowel disease.  Gut. 1983;  24 293-299
    PubMedSearch in Google Scholar
  • 3 Saverymuttu S H, Peters A M, Chadwick V S, Hodgson H J, Lavender J P. Indium leucocyte scanning in small bowel Crohn’s disease.  Gastrointest Radiol. 1983;  8 157-161
    PubMedSearch in Google Scholar
  • 4 Saverymuttu S H, Peters A M, Lavender J P, Chadwick V S, Hodgson H J. In vivo assessment of granulocyte migration in Crohn’s disease.  Gut. 1985;  26 378-383
    PubMedSearch in Google Scholar
  • 5 Roddie M E, Peters A M, Danpure H J, Osman S, Henderson B L, Lavender J P, Carroll M J, Kelly J D, Neirinckx R D. Imaging inflammation with Tc-99m hexamethyl propyleneamineoxime (HMPAO) labelled leucocytes.  Radiology. 1988;  166 767-772
    PubMedSearch in Google Scholar
  • 6 Peters A M, Roddie M E, Danpure H J, Osman S, Zacharopoulos G P, George P, Stuttle A WJ, Lavender J P. Tc-99m HMPAO labelled leucocytes: comparison with In-111-tropolonate labelled granulocytes.  Nucl Med Commun. 1988;  9 449-463
    PubMedSearch in Google Scholar
  • 7 Chisholm P M, Danpure H J, Healey G, Osman S. Cell damage resulting from the labeling of rat lymphocytes and HeLa S3 cells with In-111 oxine.  J Nucl Med. 1979;  20 1308-1311
    PubMedSearch in Google Scholar
  • 8 Thakur M L, Lavender J P, Arnol R N, Silvester D J, Segal A W. Indium-111 -labeled autologous leukocytes in man.  J Nucl Med. 1977;  18 1014-1021
    PubMedSearch in Google Scholar
  • 9 Saverymuttu S H, Peters A M, Keshavarzian A, Reavy H J, Lavender J P. The kinetics of 111-indium distribution following injection of 111-indium labelled autologous granulocytes in man.  Br J Haematol. 1985;  61 675-685
    PubMedSearch in Google Scholar
  • 10 Ussov Y u, Aktolun C, Myers M J, Jamar F, Peters A M. Granulocyte margination in bone marrow: comparison with margination in the spleen and liver.  Scand J Clin Lab Invest. 1995;  55 87-96
    PubMedSearch in Google Scholar
  • 11 Chapman P T, Haskard D O. Leukocyte adhesion molecules.  Br Med Bull. 1995;  51 296-311
    PubMedSearch in Google Scholar
  • 12 Saverymuttu S H, Peters A M, Danpure H J, Reavy H J, Osman S, Lavender J P. Lung transit of 111-indium labelled granulocytes. Relationship to labelling techniques.  Scand J Haematol. 1983;  30 151-160
    PubMedSearch in Google Scholar
  • 13 Peters A M, Saverymuttu S H, Keshavarzian A, Bell R N, Lavender J P. Splenic pooling of granulocytes.  Clin Sci. 1985;  68 283-289
    PubMedSearch in Google Scholar
  • 14 Peters A M. Just how big is the pulmonary granulocyte pool?.  Clin Sci. 1998;  94 7-19
    PubMedSearch in Google Scholar
  • 15 Kordossis T, Joseph A E, Gane J N, Bridges C E, Griffin G E. Fecal leukocytosis, indium-111-labelled autologous polymorphonuclear leukocyte abdominal scanning, and quantitative fecal indium-111 excretion in acute gastroenteritis and enteropathogen carriage.  Dig Dis Sci. 1988;  33 1383-1390
    CrossrefPubMedSearch in Google Scholar
  • 16 Hyun H, Pai E, Blend M J. Ischemic colitis: Tc-99m HMPAO leukocyte scintigraphy and correlative imaging.  Clin Nucl Med. 1998;  23 165-167
    CrossrefPubMedSearch in Google Scholar
  • 17 Saverymuttu S H, Peters A M, O’Brien C, Chadwick V S, Lavender J P, Goldman J M, Gordon-Smith E C, Hodgson H J. Indium-111 labelled autologous leucocyte scanning in gastrointestinal graft versus host disease.  Dig Dis Sci. 1986;  31 829-832
    CrossrefPubMedSearch in Google Scholar
  • 18 Ussov W Y u, Peters A M, Savill J, Pusey C D, Gaskin G, Goldman J M, Hodgson H J, Hughes J MB. Relationship between granulocyte activation, pulmonary granulocyte kinetics and alveolocapillary barrier integrity in extrapulmonary inflammatory disease.  Clin Sci. 1996;  91 329-335
    PubMedSearch in Google Scholar
  • 19 Lantto E H, Lantto T J, Vorne M. Fast diagnosis of abdominal infections and inflammations with technetium-99m-HMPAO labeled leukocytes.  J Nucl Med. 1991;  32 2029-2034
    PubMedSearch in Google Scholar
  • 20 Saverymuttu S H, Peters A M, Lavender J P. Clinical importance of enteric communication with abdominal abscesses.  Br Med J. 1985;  290 23-27
    PubMedSearch in Google Scholar
  • 21 Becker W, Pabst C, Borner W. Toxic megacolon presenting in the leukocyte scan as distended transverse bowel.  Nuklearmedizin. 1988;  27 36-37
    PubMedSearch in Google Scholar
  • 22 Puncher M R, Blower P J. Autoradiography and density gradient Separation of technetium-99m-exametazime (HMPAO) labelled leucocytes reveals selectivity for eosinophils.  Eur J Nucl Med. 1994;  21 1175-1182
    PubMedSearch in Google Scholar
  • 23 Saverymuttu S H, Peters A M, Hodgson H J, Chadwick V S, Lavender J P. Quantitative faecal 111-indium leucocyte excretion in the assessment of disease activity in Crohn’s disease.  Gastroenterology. 1983;  85 1333-1339
    PubMedSearch in Google Scholar
  • 24 Saverymuttu S H, Peters A M, Hodgson H J, Chadwick V S. Assessment of disease activity in ulcerative colitis using 111-indium labelled leucocyte faecal excretion.  Scand J Gastroenterol. 1983;  18 907-912
    PubMedSearch in Google Scholar
  • 25 Fischbach W, Becker W. Clinical relevance of activity parameters in Crohn’s disease estimated by the faecal excretion of 111-In- labeled granulocytes.  Digestion. 1991;  50 149-152
    PubMedSearch in Google Scholar
  • 26 Carpani de Kaski M, Peters A M, Knight D, Stuttle A WJ, Lavender J P, Hodgson H J. In-111 whole body retention: a new method for quantification of disease activity in inflammatory bowel disease.  J Nucl Med. 1992;  33 756-762
    PubMedSearch in Google Scholar
  • 27 Mansfield J C, Giaffer M H, Tindale W B, Holdsworth C D. Quantitative assessment of overall inflammatory bowel disease activity using labelled leucocytes: a direct comparison between indium-111 and technetium-99m HMPAO methods.  Gut. 1995;  37 679-683
    PubMedSearch in Google Scholar
  • 28 Becker W, Schomann E, Fischbach W, Borner W, Gruner K R. Comparison of Tc-99m-HMPAO and 111In-oxine labelled granulocytes in man: first clinical results.  Nucl Med Commun. 1988;  9 435-447
    PubMedSearch in Google Scholar
  • 29 Arndt J W, van der Sluys Veer A, Blok D, Griffoen G, Verspaget H W, Lamers C B, Pauwels E K. Prospective comparative study of technetium-99m-WBCs and indium-111-granulocytes for the examination of patients with inflammatory bowel disease.  J Nucl Med. 1993;  34 1052-1057
    PubMedSearch in Google Scholar
  • 30 Allan R A, Sladen G E, Bassingham S, Lazarus C, Clarke S E, Fogelman I. Comparison of simultaneous 99mTc-HMPAO and 111In-oxine labelled white cell scans in the assessment of inflammatory bowel disease.  Eur J Nucl Med. 1993;  20 195-200
    PubMedSearch in Google Scholar
  • 31 Scholmerich J, Schmidt E, Schümichen C, Billmann P, Schmidt H, Gerok W. Scintigraphic assessment of bowel involvement and disease activity in Crohn’s disease using technetium 99m-hexamethyl propylene amine oxine as leukocyte label.  Gastroenterology. 1988;  95 1287-1293
    PubMedSearch in Google Scholar
  • 32 Delgado Castro M, Lancha C, Prats E, Mitjavilla M, Abos D, Martin-Curto L M, Crespo A, Banzo J. The diagnostic value of Tc-99m human polyclonal immunoglobulin imaging compared to Tc-99m-HMPAO labeled leukocytes in inflammatory bowel disease.  Clin Nucl Med. 1997;  22 17-20
    CrossrefPubMedSearch in Google Scholar
  • 33 Mairal L, de Lima P A, Martin-Comin J, Baliellas C, Xiol X, Roca M, Ricart Y, Ramos M. Simultaneous administration of 111In-human immunoglobulin and 99mTc-HMPAO labelled leucocytes in inflammatory bowel disease.  Eur J Nucl Med. 1995;  22 664-670
    PubMedSearch in Google Scholar
  • 34 Arndt J W, Grootscholten M I, van Hogezand R A, Griffioen G, Lamers C B, Pauwels E K. Inflammatory bowel disease activity assessment using technetium-99m-HMPAO leukocytes.  Dig Dis Sci. 1997;  42 387-393
    CrossrefPubMedSearch in Google Scholar
  • 35 Middleton S J, Li D, Wharton S, Reynolds P D, Wraight E P, Hunter J O. Validation of Tc-99m-HMPAO leucocyte scintigraphy in ulcerative colitis by comparison with histology.  Br J Radiol. 1995;  68 1061-1066
    PubMedSearch in Google Scholar
  • 36 Charron M, Orenstein S R, Bhargava S. Detection of inflammatory bowel disease in pediatric patients with technetium-99m-HMPAO-labeled leukocytes.  J Nucl Med. 1994;  35 451-455
    PubMedSearch in Google Scholar
  • 37 Sciarretta G, Furno A, Mazzoni M, Basile C, Malaguti P. Technetium-99m hexamethyl propylene amine oxime granulocyte scintigraphy in Crohn’s disease: diagnostic and clinical relevance.  Gut. 1994;  35 1676-1677
    PubMedSearch in Google Scholar
  • 38 Becker W, Fischbach W, Weppler M, MosI B, Jacoby G, Borner W. Radio labelled granulocytes in inflammatory bowel disease: diagnostic possibilities and clinical indications.  Nucl Med Commun. 1988;  9 693-701
    PubMedSearch in Google Scholar
  • 39 Lantto E, Jarvi K, Krekela I, Lantto T, Taavitsainen M, Vedenkangas H, Vorne M. Technetium-99m hexamethyl propylene amine oxine leucocytes in the assessment of disease activity in inflammatory bowel disease.  Eur J Nucl Med. 1992;  19 14-18
    PubMedSearch in Google Scholar
  • 40 Almer S, Granerus G, Franzen L, Strom M. Technetium-99m scintigraphy: more accurate assessment of ulcerative colitis with exametazime- labelled leucocytes than with antigranulocyte antibodies.  Eur J Nucl Med. 1996;  23 247-255
    PubMedSearch in Google Scholar
  • 41 Stahlberg D, Veress B, Mare K, Granqvist S, Agren B, Richter S, Lofberg R. Leukocyte migration in acute colonic inflammalory bowel discase: comparison of histological assessment and Tc-99m-HMPAO labeled leukocyte scan.  Am J Gastroenterol. 1997;  92 283-288
    PubMedSearch in Google Scholar
  • 42 Stahlberg D, Mare K, Granqvist S, Agren B, Richter S, Lofberg R. Instant technetium-99m hexamethyl propylenamine oxime-labeled leukocyte scan compared with colonoscopy in early assessment of disease extent and activity in acute colilis.  Dis Colon Rectum. 1996;  39 1146-1152
    PubMedSearch in Google Scholar
  • 43 Papos M, Nagy F, Narai G, Rajtar M, Szantai G, Lang J, Csernay L. Antigranulocyte immunoscintigraphy and [99mTc-] hexamethylpropyleneamine-oxime-labeled leukocyte scintigraphy in inflammatory bowel disease.  Dig Dis Sci. 1996;  41 412-420
    PubMedSearch in Google Scholar
  • 44 Almer S, Franzen L, Peters A M, Tjadermo M, Ekberg S, Granerus G, Strom M. Do technetium-99m hexamethylpropyleneamine oxime (HMPAO) labelled leukocytes truly reflect the mucosal inflammation in paticnts with ulcerative colitis?.  Scand J Gastroenterol. 1992;  27 1031-1038
    PubMedSearch in Google Scholar
  • 45 Arndt J W, van der Sluys Veer A, Blok D, Griffioen G, Verspaget H W, Pena A S, Lamers C B, Pauwels E K. A prospective comparison of 99mTc-labeled polyclonal human immunoglobulin and 111In-granulocytes for localization of inflammatory bowel disease.  Acta Radiol. 992;  33 140-144
    PubMedSearch in Google Scholar
  • 46 Vorne M, Karhunen K, Lantto T, Mokka R, Makela P, Nikoskelainen J, Rajamaki A. Comparison of 1231 monoclonal granulocyte antibody and 99mTc- HMPAO-labelled leucocytes in the detection of inflammation.  Nucl Med Commun. 1988;  9 623-629
    PubMedSearch in Google Scholar
  • 47 Charron M, Di Lorenzo C, Kocoshis S A, Hickeson M P, Orenstein S R, Goyal A, Kahn S, Collins L. 99mTc- antigranulocyte monoclonal antibody imaging for the detection and assessment of inflammatory bowel disease newly diagnosed by colonoscopy in children.  Paed Radiol. 2001;  31 796-800
    PubMedSearch in Google Scholar
  • 48 Stokkel M P, Reigman H E, Pauwels E K. Scintigraphic head-to- head comparison between 99mTc-WBCs and 99mTc-LeukoScan in the evaluation of inflammatory bowel disease: a pilot study.  Eur J Nucl Med Mol Imaging. 2002;  29 251-254
    CrossrefPubMedSearch in Google Scholar
  • 49 Gyorke T, Duffek L, Bartfai K, Mako E, Karlinger K, Mester A, Tarjan Z. The role of nuclear medicine in inflammatory bowel disease. A review with experiences of aspecific bowel activity using immunoscintigraphy with 99mTc anti-granulocyte antibodies.  Eur J Radiol. 2000;  35 183-192
    CrossrefPubMedSearch in Google Scholar
  • 50 Neurath M F, Vehling D, Schunk K, Holtmann M, Brockmann H, Helisch A, Orth T, Schreckenberger M, Galle P R, Bartenstein P. Noninvasive assessment of Crohn ’s disease activity: a comparison of 18F-fluorodeoxyglucose positron emission tomography, hydromagnetic resonance imaging, and granulocyte scintigraphy with labeled antibodies.  Am J Gastroenterol. 2002;  97 1978-1985
    CrossrefPubMedSearch in Google Scholar
  • 51 Alonso J C, Lopez-Longo F J, Lampreave J L, Gonzalez C M, Vegazo O, Carreno L, Almoguera I. Abdominal scintigraphy using 99mTc-HMPAO-labelled leucocytes in patients with seronegative spondylarthropathies without clinical evidence of inflammatory bowel disease.  Eur J Nucl Med. 1996;  23 243-246
    PubMedSearch in Google Scholar
  • 52 Elkayam O, Paran D, Even-Sapir E, Litinsky I, Wigler I, Yaron M, Caspi D. Abdominal scintigraphy using technetium Tc 99m hexylmethylpropylene amine oxime-labeled leukocytes in patients with seronegative spondyloarthropathies.  Semin Arthr Rheum. 2002;  31 406-412
    PubMedSearch in Google Scholar
  • 53 Lionetti P, Pupi A, Veltroni M, Fonda C, Cavicchi M C, Azzari C, Falcini F. Evidence of subclinical intestinal inflammation by 99mtechnetium leukocyte scintigraphy in patients with HLA-B27 positive juvenile onset active spondyloarthropathy.  J Rheumatol. 2000;  27 1538-1541
    PubMedSearch in Google Scholar

Dr. A. M. Peters

Department of Nuclear Medicine

Box 1 70

Addenbrooke’s Hospital

Hills Road

Cambridge CB2 2QQ

United Kingdom

Phone: 0 12 23 21 71 47

Email: michael.peters@addenbrookes.nhs.uk