Download PDF
Plant Biol (Stuttg) 2004; 6(2): 165-170
DOI: 10.1055/s-2003-44719
Original Paper

Georg Thieme Verlag Stuttgart · New York

Positive and Negative Tropic Curvature Induced by Microbeam Irradiation of Protonemal Tip Cells of the Moss Ceratodon purpureus

T. Lamparter1 , T. Kagawa2 , G. Brücker1 , M. Wada2 , 3
  • 1Institut für Biologie, Pflanzenphysiologie, Freie Universität Berlin, Berlin, Germany
  • 2National Institute of Basic Biology, Nishigonaka 38, Myodaiji, Okazaki, Aichi 444-8585, Japan
  • 3Department of Biology, Tokyo Metropolitan University, Minami-Ohsawa, 1-1, Hachioji-shi, Tokyo 192-0397, Japan
Further Information

Publication History

Publication Date:
26 March 2004 (online)

   Permissions and Reprints

Abstract

The photoreceptor phytochrome mediates tropic responses in protonemata of the moss Ceratodon purpureus. Under standard conditions the tip cells grow towards unilateral red light, or perpendicular to the electrical vector of polarized light. In this study the response of tip cells to partial irradiation of the apical region was analysed using a microbeam apparatus. The fluence response curve gave an unexpected pattern: whereas a 15-min microbeam with light intensities around 3 µmol m-2 s-1 induced a growth curvature towards the irradiated side, higher light intensities around 100 µmol m-2 s-1 caused a negative response, the cells grew away from the irradiated side. This avoidance response is explained by two effects: the light intensity is high enough to induce photoconversion into the active Pfr form of phytochrome, not only on the irradiated but also on the non-irradiated side by stray light. At the same time, the strong light on the irradiated side acts antagonistically to Pfr. As a result of this inhibition, the growth direction is moved to the light-avoiding side. Such a Pfr-independent mechanism might be important for the phototropic response to distinguish between the light-directed and light-avoiding side under unilateral light.

Key words

Action dichroism - light direction sensing - phototropism - phytochrome.

References

  • 1 Briggs W. R., Christie J. M.. Phototropins 1 and 2: versatile plant blue-light receptors.  Trends Plant Sci.. (2002);  7 204-210
    CrossrefPubMedSearch in Google Scholar
  • 2 Brücker G., Zeidler M., Kohchi T., Hartmann E., Lamparter T.. Microinjection of heme oxygenase genes rescues phytochrome-chromophore-deficient mutants of the moss Ceratodon purpureus. .  Planta. (2000);  210 529-535
    CrossrefPubMedSearch in Google Scholar
  • 3 Butler W. L., Hendricks S. B., Siegelman H. W.. Action spectra of phytochrome in vitro. .  Photochem. Photobiol.. (1964);  3 521-528
    PubMedSearch in Google Scholar
  • 4 Cove D. J., Quatrano R. S., Hartmann E.. The alignment of the axis of asymmetry in regenerating protoplasts of the moss, Ceratodon purpureus, is determined independently of axis polarity.  Development. (1996);  122 371-379
    PubMedSearch in Google Scholar
  • 5 Esch H., Hartmann E., Cove D., Wada M., Lamparter T.. Phytochrome-controlled phototropism of protonemata of the moss Ceratodon purpureus: physiology of wild type and class 2 ptr mutants.  Planta. (1999);  209 290-298
    CrossrefPubMedSearch in Google Scholar
  • 6 Hartmann E., Klingenberg B., Bauer L.. Phytochrome mediated phototropism in protonemata of the moss Ceratodon purpureus BRID.  Photochem. Photobiol.. (1983);  38 599-603
    PubMedSearch in Google Scholar
  • 7 Hartmann E., Weber M.. Storage of the phytochrome-mediated phototropic stimulus of moss protonematal cells.  Planta. (1988);  175 39-49
    CrossrefPubMedSearch in Google Scholar
  • 8 Iino M., Shitanishi K., Kadota A., Wada M.. Phytochrome mediated phototropism in Adiantum protonemata - I. Phototropism as a function of the lateral Pfr gradient.  Photochem. Photobiol.. (1990);  51 469-476
    PubMedSearch in Google Scholar
  • 9 Kagawa T., Kadota A., Wada M.. Phytochrome mediated photoorientation of chloroplasts in protonemal cells of the fern Adiantum can be induced by brief irradiation with red light.  Plant Cell Physiol.. (1994);  35 371-377
    PubMedSearch in Google Scholar
  • 10 Kendrick R. E., Kronenberg G. H. M.. Photomorphogenesis in Plants, 2nd edition. Dordrecht; Kluwer Academic Publishers (1994)
    Search in Google Scholar
  • 11 Kern V. D., Sack F. D.. Irradiance-dependent regulation of gravitropism by red light in protonemata.  Planta. (1999);  1999 299-307
    PubMedSearch in Google Scholar
  • 12 Kraml M.. Light direction and polarisation. Kendrick, R. E. and Kronenberg, G. H. M., eds. Photomorphogenesis in Plants. Dordrecht; Kluwer Academic Publishers (1994): 417-446
    Search in Google Scholar
  • 13 Lamparter T., Podlowski S., Mittmann F., Schneider-Poetsch H., Hartmann E., Hughes J.. Phytochrome from protonemal tissue of the moss Ceratodon purpureus. .  J. Plant Physiol.. (1995);  147 426-434
    PubMedSearch in Google Scholar
  • 14 Mancinelli A.. The physiology of phytochrome action. Kendrick, R. E. and Kronenberg, G. H. M., eds. Photomorphogenesis in Plants, 2nd edition. Dordrecht; Kluwer Academic Publishers (1994): 211-269
    Search in Google Scholar
  • 15 Smith H.. Phytochromes and light signal perception by plants - an emerging synthesis.  Nature. (2000);  407 585-591
    CrossrefPubMedSearch in Google Scholar
  • 16 Wada M., Sugai M.. Photobiology of ferns. Kendrick, R. E. and Kronenberg, G. H. M., eds. Photomorphogenesis in Plants. Dordrecht; Kluwer Academic Publishers (1994): 783-803
    Search in Google Scholar
  • 17 Zeidler M., Lamparter T., Hughes J., Hartmann E., Remberg A., Braslavsky S., Schaffner K., Gärtner W.. Recombinant phytochrome of the moss Ceratodon purpureus: heterologous expression and kinetic analysis of Pr → Pfr conversion.  Photochem. Photobiol.. (1998);  68 857-863
    CrossrefPubMedSearch in Google Scholar

T. Lamparter

Institut für Biologie, Pflanzenphysiologie
Freie Universität Berlin

Königin-Luise-Straße 12 - 16

14195 Berlin

Germany

Email: lamparte@zedat.fu-berlin.de

Section Editor: G. Thiel