Molecular Genetic Survey of European Mistletoe (Viscum album) Subspecies with Allele-Specific and dCAPS Type Markers Specific for Chloroplast and Nuclear DNA Sequences

Abstract

The qualitative and quantitative content of mistletoe metabolites, and bioactivity of extracts is related to the subspecies of Viscum album L. These were indicated to be genetically distinct and host specific. We aimed to check (i) whether the specificity is strict and (ii) how frequently hybridization occurs among the subspecies. We designed two sets of allele-specific and dCAPS molecular genetic markers that would facilitate identification of Viscum album L. subspecies and their hybrid derivatives on the basis of chloroplast trnH(GUG)-trnK(UUU) and nuclear rDNA ITS1&2 sequences. Out of 118 plants surveyed, 103 displayed characteristics that confirmed strict host specificity of the subspecies, in addition, the results were compliant between nuclear and chloroplast markers showing no indication of hybridization among subspecies. From 15 samples that showed deviations from this model 13 came from the Mediterranean Sea basin, and only two originated from Central and Western Europe.

Abbreviations

dCAPS:derived Cleaved Amplified Polymorphic Sequence

ITS1&2:Internal Transcribed Spacers 1&2

MAMA:Mismatch Amplification Mutation Assay

Viscum album - Loranthaceae - allele-specific - dCAPS - host-specificity - hybridization

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